scholarly journals Microsatellite markers for study of the invasive species Rumex alpinus (Polygonaceae)

2018 ◽  
Vol 6 (1) ◽  
pp. e1017 ◽  
Author(s):  
Mária Šurinová ◽  
Andrea Jarošová ◽  
Zuzana Münzbergová
Keyword(s):  
Invasive Species ◽  
Microsatellite Markers ◽  
Rumex Alpinus

scholarly journals Transferability of Microsatellite Markers Developed in Oenothera spp. to the Invasive Species Oenothera drummondii Hook. (Onagraceae)

Diversity ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 387
Author(s):  
Raquel Hernández-Espinosa ◽  
Jorge González-Astorga ◽  
Alejandro Espinosa de los Monteros ◽  
Dánae Cabrera-Toledo ◽  
Juan B. Gallego-Fernández
Keyword(s):  
Invasive Species ◽  
North Carolina ◽  
Microsatellite Markers ◽  
Coastal Dunes ◽  
The United States ◽  
Null Alleles ◽  
Genetic Studies ◽  
Native Populations ◽  
Hardy Weinberg Equilibrium ◽  
Future Population

Oenothera drummondii Hook. (Onagraceae) has life-history traits that make it an invasive species. Native populations are distributed along the coastal dunes from North Carolina in the United States to Tabasco in the Gulf of Mexico. It has been reported as an invasive species in Spain, Israel, and China, where this species can successfully colonize and dominate if the environmental conditions are appropriate. In South Africa, Australia, New Zealand, and France, it is reported to be naturalized. In this study, 28 microsatellite markers developed for other Oenothera species were evaluated for cross-amplification in O. drummondii. Nine primers showed consistent amplification and were polymorphic. Polymorphism was assessed in three populations from both native and invaded areas. Results indicated generalized low genetic variability. Three loci showed significant deviations from the Hardy Weinberg equilibrium, associated with null alleles’ presence. The observed heterozygosity and inbreeding coefficient reflected a generalized excess of homozygotes, particularly in the invaded population “El Dique”, likely due to allele fixation. High genetic differentiation was found between the three populations. These results highlight the accuracy of these markers for future population genetic studies in O. drummondii.

scholarly journals The Endangered White Sands pupfish (Cyprinodon tularosa) genome reveals low diversity and heterogenous patterns of differentiation

2020 ◽  
Author(s):  
Andrew Black ◽  
Janna Willoughby ◽  
Anna Brüniche-Olsen ◽  
Brian Pierce ◽  
Andrew DeWoody
Keyword(s):  
Invasive Species ◽  
Microsatellite Markers ◽  
Related Species ◽  
Draft Genome ◽  
Genomic Diversity ◽  
Chemical Pollution ◽  
Cyprinodon Tularosa ◽  
White Sands Pupfish ◽  
White Sands

The White Sands pupfish (Cyprinodon tularosa), endemic to New Mexico in Southwestern North America, is of conservation concern due in part to invasive species, chemical pollution, and groundwater withdrawal. Herein, we developed a high quality draft reference genome and use it to provide biological insights into the evolution and conservation of C. tularosa. Specifically, we localized microsatellite markers previously used to demarcate Evolutionary Significant Units, evaluated the possibility of introgression into the C. tularosa genome, and compared genomic diversity among related species. The de novo assembly of PacBio Sequel II error-corrected reads resulted in a 1.08Gb draft genome with a contig N50 of 1.4Mb and 25,260 annotated protein coding genes, including 95% of the expected Actinopterigii conserved orthologs. Many of the previously described C. tularosa microsatellite markers fell within or near genes and exhibited a pattern of increased heterozygosity near genic areas compared to those in intergenic regions. Genetic distances between C. tularosa and the widespread invasive species C. variegatus, which diverged ~1.6-4.7 MYA, were 0.027 (nuclear) and 0.022 (mitochondrial). Nuclear alignments revealed putative tracts of introgression that merit further investigation. Genome-wide heterozygosity was markedly lower in C. tularosa compared to estimates from related species, likely because of smaller long-term effective population sizes constrained by their isolated and limited habitat. These population inferences, generated from our new genome assembly, provide insights into the long term and contemporary White Sands pupfish populations that are integral to future management efforts.

scholarly journals Microsatellite Markers for the Invasive Species Bidens alba (Asteraceae)

2014 ◽  
Vol 2 (5) ◽  
pp. 1400008 ◽  
Author(s):  
Yong-Bin Lu ◽  
Dong-Ling Huang ◽  
Xie Wang ◽  
Zheng-Jun Wu ◽  
Shao-Qing Tang
Keyword(s):  
Invasive Species ◽  
Microsatellite Markers

A panel of mono- and dinucleotide microsatellite markers is required for reliable determination of the MSI status in colorectal cancer

2001 ◽  
Vol 120 (5) ◽  
pp. A599-A599
Author(s):  
C ARNOLD ◽  
A GOEL ◽  
J CARETHERS ◽  
L WASSERMAN ◽  
C COMPTON ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Microsatellite Markers ◽  
Reliable Determination
Sign in / Sign up

Export Citation Format

Share Document