An in vitro evaluation of Genipin‐crosslinked and Hypericum perforatum incorporated novel membranes for skin tissue engineering applications

The fabrication of biocompatible and biodegradable scaffolds which mimic the native extracellular matrix of tissues to promote cell adhesion and growth is emphasized recently. Many polymers have been utilized in scaffold fabrication, but there is still a need to fabricate hydrophilic nanosized fibrous scaffolds with an appropriate degradation rate for skin tissue engineering applications. In this study, nanofibrous scaffolds of a biodegradable synthetic polymer, polycaprolactone (PCL), and blends of PCL with a natural polymer, gelatine (Ge), in three different compositions: 85 : 15, 70 : 30, and 50 : 50 were fabricated via an electrospinning technique. The nanofibrous scaffold prepared from 14% w/v PCL/Ge (70 : 30) exhibited more balanced properties of homogeneous nanofibres with an average fibre diameter of 155.60 ± 41.13 nm, 83% porosity, and surface roughness of 176.27 ± 2.53 nm.In vitrocell culture study using human skin fibroblasts (HSF) demonstrated improved cell attachment with a flattened morphology on the PCL/Ge (70 : 30) nanofibrous scaffold and accelerated proliferation on day 3 compared to the PCL nanofibrous scaffold. These results show that the PCL/Ge (70 : 30) nanofibrous scaffold was more favourable and has the potential to be a promising scaffold for skin tissue engineering applications.

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In vitro degradation study of novel HEC/PVA/collagen nanofibrous scaffold for skin tissue engineering applications

Polymer Degradation and Stability ◽

10.1016/j.polymdegradstab.2014.10.017 ◽

2014 ◽

Vol 110 ◽

pp. 473-481 ◽

Cited By ~ 37

Author(s):

Farah Hanani Zulkifli ◽

Fathima Shahitha Jahir Hussain ◽

Mohammad Syaiful Bahari Abdull Rasad ◽

Mashitah Mohd Yusoff

Keyword(s):

Tissue Engineering ◽

Nanofibrous Scaffold ◽

Skin Tissue ◽

Engineering Applications ◽

In Vitro Degradation ◽

Degradation Study ◽

Skin Tissue Engineering

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Needleless electrospinning of PVP/PGS fibrous scaffolds for skin tissue engineering applications

10.31224/osf.io/xs64k ◽

2018 ◽

Cited By ~ 1

Author(s):

Antonios Keirouz ◽

Giuseppino Fortunato ◽

Anthony Callanan ◽

Norbert Radacsi

Keyword(s):

Tissue Engineering ◽

Tensile Modulus ◽

Dermal Fibroblasts ◽

Skin Tissue ◽

Skin Substitute ◽

Electrospinning Technique ◽

Skin Tissue Engineering ◽

Needleless Electrospinning ◽

High Concentrations

Scaffolds and implants used for tissue engineering need to be adapted for their mechanical properties with respect to their environment within the human body. Therefore, a novel composite for skin tissue engineering is presented by use of blends of Poly(vinylpyrrolidone) (PVP) and Poly(glycerol sebacate) (PGS) were fabricated via the needleless electrospinning technique. The formed PGS/PVP blends were morphologically, thermochemically and mechanically characterized. The morphology of the developed fibers related to the concentration of PGS, with high concentrations of PGS merging the fibers together plasticizing the scaffold. The tensile modulus appeared to be affected by the concentration of PGS within the blends, with an apparent decrease in the elastic modulus of the electrospun mats and an exponential increase of the elongation at break. Ultraviolet (UV) crosslinking of PGS/PVP significantly decreased and stabilized the wettability of the formed fiber mats, as indicated by contact angle measurements. In vitro examination showed good viability and proliferation of human dermal fibroblasts over the period of a week. The present findings provide important insights for tuning the elastic properties of electrospun material by incorporating this unique elastomer, as a promising future candidate for skin substitute constructs.

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075 Generation and quantification of oxidized squalene to develop an acne testing model in vitro based on skin tissue engineering

Journal of Investigative Dermatology ◽

10.1016/j.jid.2016.06.093 ◽

2016 ◽

Vol 136 (9) ◽

pp. S173 ◽

Cited By ~ 1

Author(s):

N. Esselin ◽

C. Capallere ◽

C. Meyrignac ◽

C. Plaza ◽

C. Coquet ◽

...

Keyword(s):

Tissue Engineering ◽

Skin Tissue ◽

Testing Model ◽

Skin Tissue Engineering

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Gamma radiation-induced grafting of n-hydroxyethyl acrylamide onto poly(3-hydroxybutyrate): A companion study on its polyurethane scaffolds meant for potential skin tissue engineering applications

Materials Science and Engineering C ◽

10.1016/j.msec.2020.111176 ◽

2020 ◽

Vol 116 ◽

pp. 111176

Author(s):

Eric Ivan Ochoa-Segundo ◽

Maykel González-Torres ◽

Alejandro Cabrera-Wrooman ◽

Roberto Sánchez-Sánchez ◽

Blanca Margarita Huerta-Martínez ◽

...

Keyword(s):

Tissue Engineering ◽

Gamma Radiation ◽

Skin Tissue ◽

Engineering Applications ◽

Skin Tissue Engineering ◽

Radiation Induced ◽

Radiation Induced Grafting

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