P75 and S100 gene expression induced by cell‐imprinted substrate and beta‐carotene to nerve tissue engineering

2021 ◽  
Vol 138 (26) ◽  
pp. 50624
Author(s):  
Sadaf Dadashkhan ◽  
Shiva Irani ◽  
Shahin Bonakdar ◽  
Behafarid Ghalandari
Keyword(s):  
Gene Expression ◽  
Tissue Engineering ◽  
Beta Carotene ◽  
Nerve Tissue ◽  
Nerve Tissue Engineering ◽  
S100 Gene

scholarly journals Tailoring Nano-Porous Surface of Aligned Electrospun Poly (L-Lactic Acid) Fibers for Nerve Tissue Engineering

2021 ◽  
Vol 22 (7) ◽  
pp. 3536
Author(s):  
Hongyun Xuan ◽  
Biyun Li ◽  
Feng Xiong ◽  
Shuyuan Wu ◽  
Zhuojun Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Bacterial Adhesion ◽  
Bacterial Colonization ◽  
Cellular Responses ◽  
Surface Structures ◽  
Porous Surface ◽  
Nerve Tissue ◽  
Proliferation And Differentiation ◽  
Nerve Tissue Engineering

Despite the existence of many attempts at nerve tissue engineering, there is no ideal strategy to date for effectively treating defective peripheral nerve tissue. In the present study, well-aligned poly (L-lactic acid) (PLLA) nanofibers with varied nano-porous surface structures were designed within different ambient humidity levels using the stable jet electrospinning (SJES) technique. Nanofibers have the capacity to inhibit bacterial adhesion, especially with respect to Staphylococcus aureus (S. aureus). It was noteworthy to find that the large nano-porous fibers were less detrimentally affected by S. aureus than smaller fibers. Large nano-pores furthermore proved more conducive to the proliferation and differentiation of neural stem cells (NSCs), while small nano-pores were more beneficial to NSC migration. Thus, this study concluded that well-aligned fibers with varied nano-porous surface structures could reduce bacterial colonization and enhance cellular responses, which could be used as promising material in tissue engineering, especially for neuro-regeneration.

scholarly journals Cyclic tensile stimulation enrichment of Schwann cell-laden auxetic hydrogel scaffolds towards peripheral nerve tissue engineering

2020 ◽  
Vol 195 ◽  
pp. 108982 ◽  
Author(s):  
Yi-Wen Chen ◽  
Kan Wang ◽  
Chia-Che Ho ◽  
Chia-Tze Kao ◽  
Hooi Yee Ng ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Schwann Cell ◽  
Peripheral Nerve ◽  
Nerve Tissue ◽  
Hydrogel Scaffolds ◽  
Nerve Tissue Engineering

scholarly journals Nerve tissue engineering using blends of poly(3-hydroxyalkanoates) for peripheral nerve regeneration

2015 ◽  
Vol 15 (6) ◽  
pp. 612-621 ◽  
Author(s):  
Lorena R. Lizarraga-Valderrama ◽  
Rinat Nigmatullin ◽  
Caroline Taylor ◽  
John W. Haycock ◽  
Frederik Claeyssens ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Nerve Tissue ◽  
Nerve Tissue Engineering

scholarly journals Vascularization Strategies for Peripheral Nerve Tissue Engineering

2018 ◽  
Vol 301 (10) ◽  
pp. 1657-1667 ◽  
Author(s):  
Papon Muangsanit ◽  
Rebecca J. Shipley ◽  
James B. Phillips
Keyword(s):  
Tissue Engineering ◽  
Peripheral Nerve ◽  
Nerve Tissue ◽  
Nerve Tissue Engineering

In Vitro Evaluation of the Growth and Migration of Schwann Cells on Electrospun Silk Fibroin Nanofibers

2011 ◽  
Vol 175-176 ◽  
pp. 220-223 ◽  
Author(s):  
Ai Jun Hu ◽  
Bao Qi Zuo ◽  
Feng Zhang ◽  
Qing Lan ◽  
Huan Xiang Zhang
Keyword(s):  
Tissue Engineering ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Silk Fibroin ◽  
Nerve Tissue ◽  
Nerve Tissue Engineering ◽  
And Migration ◽  
Silk Fibroin Nanofibers

Schwann cells (SCs) are primary structural and functional cells in peripheral nervous system and play a crucial role in peripheral nerve regeneration. Current challenge in peripheral nerve tissue engineering is to produce an implantable scaffold capable of bridging long nerve gaps and assist Scs in directing the growth of regenerating axons in nerve injury recovery. Electrospun silk fibroin nanofibers, fabricated for the cell culture in vitro, can provide such experiment support. Silk fibroin scaffolds (SFS) were fabricated with formic acid (FA), and the average fiber diameter was 305 ± 24 nm. The data from microscopic, immunohistochemical and scanning electron micrograph confirmed that the scaffold was beneficial to the adherence, proliferation and migration of SCs without exerting any significant cytotoxic effects on their phenotype. Thus, providing an experimental foundation accelerated the formation of bands of Bünger to enhance nerve regeneration. 305 nm SFS could be a candidate material for nerve tissue engineering.

Preparation of Porous Multi-Channeled Chitosan Conduits for Nerve Tissue Engineering

2005 ◽  
Vol 288-289 ◽  
pp. 27-30 ◽  
Author(s):  
Q. Ao ◽  
A.J. Wang ◽  
W.L. Cao ◽  
C. Zhao ◽  
Ya Dong Gong ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stainless Steel ◽  
Spinal Cord Injuries ◽  
Neuroblastoma Cells ◽  
Nerve Tissue ◽  
Tissue Engineering Scaffolds ◽  
Nerve Conduits ◽  
Porous Chitosan ◽  
Nerve Tissue Engineering ◽  
Chitosan Gel

A new method to fabricate porous chitosan nerve conduits with multi-channels was described. A uniquely designed mold was composed of 7-50 stainless steel needles and a set of plastic pedestals. Porous or imperforate chitosan tubes with 2-5mm inner diameter and 0.2-1.0 mm wall thickness were made firstly. The chitosan tubes were injected with 3% chitosan gel. The stainless steel needles longitudinally perforated through the chitosan tubes filled with chitosan gel, and the plastic pedestals were used to fix the needles. Lyophilization was used to finish fabrication. The diameter of channels was 0.2-0.4mm. Swelling property and biodegradability of Multi-channeled chitosan conduits were investigated. Wright’s staining and scanning electron microscope (SEM) were used to observe spread and proliferation of Neuroblastoma cells (N2A, mouse) on the conduits. It is promising that the porous chitosan nerve conduits with multi-channels are used as nerve tissue engineering scaffolds in repair of peripheral nerve and spinal cord injuries.

Electrospun polycaprolactone/chitosan scaffolds for nerve tissue engineering: physicochemical characterization and Schwann cell biocompatibility

2016 ◽  
Vol 12 (1) ◽  
pp. 015008 ◽  
Author(s):  
Ena Bolaina-Lorenzo ◽  
Cristina Martínez-Ramos ◽  
Manuel Monleón-Pradas ◽  
Wilberth Herrera-Kao ◽  
Juan V Cauich-Rodríguez ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Schwann Cell ◽  
Physicochemical Characterization ◽  
Nerve Tissue ◽  
Nerve Tissue Engineering ◽  
Chitosan Scaffolds ◽  
Cell Biocompatibility

Novel electrospun conduit based on polyurethane/collagen enhanced by nanobioglass for peripheral nerve tissue engineering

2022 ◽  
pp. 1-15
Author(s):  
Somayeh Tofighi Nasab ◽  
Nasim Hayati Roodbari ◽  
Vahabodin Goodarzi ◽  
Hossein Ali Khonakdar ◽  
Kourosh Mansoori ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Peripheral Nerve ◽  
Nerve Tissue ◽  
Nerve Tissue Engineering
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