Biocomposite scaffolds for 3D cell culture: Propolis enriched polyvinyl alcohol nanofibers favoring cell adhesion

2020 ◽  
pp. 50287
Author(s):  
Rumeysa Bilginer ◽  
Dilce Ozkendir‐Inanc ◽  
Umit Hakan Yildiz ◽  
Ahu Arslan‐Yildiz
Keyword(s):  
Cell Culture ◽  
Cell Adhesion ◽  
Polyvinyl Alcohol ◽  
3D Cell Culture

Effects of Surface Texturing on Cell Adhesion for Artificial Joints

2004 ◽  
Vol 823 ◽  
Author(s):  
B. Shi ◽  
A. Fairchild ◽  
Z. Kleine ◽  
T. Kuhn ◽  
H. Liang
Keyword(s):  
Cell Culture ◽  
Cell Adhesion ◽  
Polyvinyl Alcohol ◽  
Surface Texturing ◽  
Surface Structures ◽  
Artificial Joints ◽  
Profound Impact ◽  
Joint Implants ◽  
Materials Used ◽  
Subsequent Failure

AbstractOne of the major issues of joint implants is their loss (detachment) and subsequent failure after a limited number of years in application. Understanding and improving cell adhesion to implanting materials to extend lifespan, lubrication, and self-regeneration properties are therefore substantially important. In this research, we investigate effects of surface properties, such as wettabilty and texture on cell culture for the purpose of biotribological applications. Materials used are polyurethane, polyvinyl alcohol, and glass. Surface analysis indicates that surface structures have profound impact on cells growth.

Surface modification of a polyvinyl alcohol sponge with functionalized boronic acids to develop porous materials for multicolor emission, chemical sensing and 3D cell culture

2017 ◽  
Vol 53 (25) ◽  
pp. 3563-3566 ◽  
Author(s):  
Ryuhei Nishiyabu ◽  
Shunsuke Iizuka ◽  
Saika Minegishi ◽  
Hiroaki Kitagishi ◽  
Yuji Kubo
Keyword(s):  
Cell Culture ◽  
Surface Modification ◽  
Polyvinyl Alcohol ◽  
Porous Materials ◽  
Chemical Sensing ◽  
3D Cell Culture ◽  
Boronic Acids

Surface modification of a polyvinyl alcohol sponge with functionalized boronic acids led to the formation of porous materials applicable for multicolor emission, chemical sensing and 3D cell culture.

Going beyond RGD: screening of a cell-adhesion peptide library in 3D cell culture

2020 ◽  
Vol 15 (5) ◽  
pp. 055033
Author(s):  
Mariah Sarwat ◽  
Denver C Surrao ◽  
Nick Huettner ◽  
James A St John ◽  
Tim R Dargaville ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Adhesion ◽  
3D Cell Culture ◽  
Peptide Library ◽  
A Cell

A Novel Cheap and Easy to Handle Protein Hydrogel for 3D Cell Culture Applications: A High Stability Matrix with Tunable Elasticity and Cell Adhesion Properties

2016 ◽  
Vol 1 (7) ◽  
pp. 1353-1360 ◽  
Author(s):  
Nicholas Bodenberger ◽  
Patrick Paul ◽  
Dennis Kubiczek ◽  
Paul Walther ◽  
Kay-Eberhard Gottschalk ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Adhesion ◽  
High Stability ◽  
3D Cell Culture ◽  
Adhesion Properties ◽  
Stability Matrix

scholarly journals Applications of Biomaterials in 3D Cell Culture and Contributions of 3D Cell Culture to Drug Development and Basic Biomedical Research

2021 ◽  
Vol 22 (5) ◽  
pp. 2491
Author(s):  
Yujin Park ◽  
Kang Moo Huh ◽  
Sun-Woong Kang
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Culture ◽  
3D Cell Culture ◽  
Accurate Method ◽  
New Drugs ◽  
Toxicity Screening ◽  
Cellular Functions ◽  
Toxicity Of Drugs ◽  
External Stimuli

The process of evaluating the efficacy and toxicity of drugs is important in the production of new drugs to treat diseases. Testing in humans is the most accurate method, but there are technical and ethical limitations. To overcome these limitations, various models have been developed in which responses to various external stimuli can be observed to help guide future trials. In particular, three-dimensional (3D) cell culture has a great advantage in simulating the physical and biological functions of tissues in the human body. This article reviews the biomaterials currently used to improve cellular functions in 3D culture and the contributions of 3D culture to cancer research, stem cell culture and drug and toxicity screening.

scholarly journals SpheroidPicker for automated 3D cell culture manipulation using deep learning

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Istvan Grexa ◽  
Akos Diosdi ◽  
Maria Harmati ◽  
Andras Kriston ◽  
Nikita Moshkov ◽  
...  
Keyword(s):  
Cell Culture ◽  
Precision Medicine ◽  
High Throughput Screening ◽  
Standard Sample ◽  
Ex Vivo ◽  
Low Cost ◽  
3D Cell Culture ◽  
Syringe Pump ◽  
Experimental Conditions ◽  
The Past

AbstractRecent statistics report that more than 3.7 million new cases of cancer occur in Europe yearly, and the disease accounts for approximately 20% of all deaths. High-throughput screening of cancer cell cultures has dominated the search for novel, effective anticancer therapies in the past decades. Recently, functional assays with patient-derived ex vivo 3D cell culture have gained importance for drug discovery and precision medicine. We recently evaluated the major advancements and needs for the 3D cell culture screening, and concluded that strictly standardized and robust sample preparation is the most desired development. Here we propose an artificial intelligence-guided low-cost 3D cell culture delivery system. It consists of a light microscope, a micromanipulator, a syringe pump, and a controller computer. The system performs morphology-based feature analysis on spheroids and can select uniform sized or shaped spheroids to transfer them between various sample holders. It can select the samples from standard sample holders, including Petri dishes and microwell plates, and then transfer them to a variety of holders up to 384 well plates. The device performs reliable semi- and fully automated spheroid transfer. This results in highly controlled experimental conditions and eliminates non-trivial side effects of sample variability that is a key aspect towards next-generation precision medicine.

scholarly journals Identification of Novel FNIN2 and FNIN3 Fibronectin-Derived Peptides That Promote Cell Adhesion, Proliferation and Differentiation in Primary Cells and Stem Cells

2021 ◽  
Vol 22 (6) ◽  
pp. 3042
Author(s):  
Eun Ju Lee ◽  
Khurshid Ahmad ◽  
Shiva Pathak ◽  
SunJu Lee ◽  
Mohammad Hassan Baig ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Cell Adhesion ◽  
3D Culture ◽  
Human Mesenchymal Stem Cells ◽  
Cell Types ◽  
Primary Cells ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Promote Cell Adhesion

In recent years, a major rise in the demand for biotherapeutic drugs has centered on enhancing the quality and efficacy of cell culture and developing new cell culture techniques. Here, we report fibronectin (FN) derived, novel peptides fibronectin-based intergrin binding peptide (FNIN)2 (18-mer) and FNIN3 (20-mer) which promote cell adhesion proliferation, and the differentiation of primary cells and stem cells. FNIN2 and 3 were designed based on the in silico interaction studies between FN and its receptors (integrin α5β1, αvβ3, and αIIbβ3). Analysis of the proliferation of seventeen-cell types showed that the effects of FNINs depend on their concentration and the existence of expressed integrins. Significant rhodamine-labeled FNIN2 fluorescence on the membranes of HeLa, HepG2, A498, and Du145 cells confirmed physical binding. Double coating with FNIN2 or 3 after polymerized dopamine (pDa) or polymerized tannic acid (pTA) precoating increased HBEpIC cell proliferation by 30–40 percent, suggesting FNINs potently affect primary cells. Furthermore, the proliferation of C2C12 myoblasts and human mesenchymal stem cells (MSCs) treated with FNINs was significantly increased in 2D/3D culture. FNINs also promoted MSC differentiation into osteoblasts. The results of this study offer a new approach to the production of core materials (e.g., cell culture medium components, scaffolds) for cell culture.

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