Production of silk fibroin nanoparticles using ionic liquids and high-power ultrasounds

2014 ◽  
pp. n/a-n/a ◽  
Author(s):  
Antonio Abel Lozano-Pérez ◽  
Mercedes García Montalbán ◽  
Salvador David Aznar-Cervantes ◽  
Francesca Cragnolini ◽  
José Luis Cenis ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Silk Fibroin ◽  
High Power ◽  
Silk Fibroin Nanoparticles

scholarly journals On the Secondary Structure of Silk Fibroin Nanoparticles Obtained Using Ionic Liquids: An Infrared Spectroscopy Study

Polymers ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1294
Author(s):  
Guzmán Carissimi ◽  
Cesare M. Baronio ◽  
Mercedes G. Montalbán ◽  
Gloria Víllora ◽  
Andreas Barth
Keyword(s):  
Ionic Liquids ◽  
Secondary Structure ◽  
Infrared Spectrum ◽  
Silk Fibroin ◽  
Liquid Solution ◽  
Power Ultrasound ◽  
Sheet Structure ◽  
Silk Fibroin Nanoparticles ◽  
Ionic Liquid Solution ◽  
Β Sheet

Silk fibroin from Bombyx mori caterpillar is an outstanding biocompatible polymer for the production of biomaterials. Its impressive combination of strength, flexibility, and degradability are related to the protein’s secondary structure, which may be altered during the manufacture of the biomaterial. The present study looks at the silk fibroin secondary structure during nanoparticle production using ionic liquids and high-power ultrasound using novel infrared spectroscopic approaches. The infrared spectrum of silk fibroin fibers shows that they are composed of 58% β-sheet, 9% turns, and 33% irregular and/or turn-like structures. When fibroin was dissolved in ionic liquids, its amide I band resembled that of soluble silk and no β-sheet absorption was detected. Silk fibroin nanoparticles regenerated from the ionic liquid solution exhibited an amide I band that resembled that of the silk fibers but had a reduced β-sheet content and a corresponding higher content of turns, suggesting an incomplete turn-to-sheet transition during the regeneration process. Both the analysis of the experimental infrared spectrum and spectrum calculations suggest a particular type of β-sheet structure that was involved in this deficiency, whereas the two other types of β-sheet structure found in silk fibroin fibers were readily formed.

scholarly journals Silk fibroin nanoparticles enhance quercetin immunomodulatory properties in DSS-induced mouse colitis

2021 ◽  
pp. 120935
Author(s):  
Patricia Diez-Echave ◽  
Antonio Jesús Ruiz-Malagón ◽  
José Alberto Molina-Tijeras ◽  
Laura Hidalgo-García ◽  
Teresa Vezza ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Silk Fibroin Nanoparticles ◽  
Immunomodulatory Properties

Fabrication of biocompatible porous SAIB/Silk fibroin scaffolds using ionic liquids

2021 ◽  
Author(s):  
Cristiana Gonçalves ◽  
Joana Margarida Gomes ◽  
F. Raquel Maia ◽  
Hajer Radhouani ◽  
Simone Dos Santos Silva ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Ionic Liquids ◽  
Silk Fibroin ◽  
Innovative Methodology

Sucrose acetate isobutyrate (SAIB) is a highly viscous hydrogel and presents many challenges for processing when its application in tissue engineering is envisioned. Herein, is proposed an innovative methodology by...

scholarly journals Formulation, characterization, and cellular toxicity assessment of tamoxifen-loaded silk fibroin nanoparticles in breast cancer

Drug Delivery ◽  
2021 ◽  
Vol 28 (1) ◽  
pp. 1626-1636
Author(s):  
Afrasim Moin ◽  
Shahid Ud Din Wani ◽  
Riyaz Ali Osmani ◽  
Amr S. Abu Lila ◽  
El-Sayed Khafagy ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Silk Fibroin ◽  
Toxicity Assessment ◽  
Cellular Toxicity ◽  
Silk Fibroin Nanoparticles

Targeted Delivery System Based on Gemcitabine-Loaded Silk Fibroin Nanoparticles for Lung Cancer Therapy

2017 ◽  
Vol 9 (37) ◽  
pp. 31600-31611 ◽  
Author(s):  
Fatemeh Mottaghitalab ◽  
Melika Kiani ◽  
Mehdi Farokhi ◽  
Subhas C. Kundu ◽  
Rui L. Reis ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Therapy ◽  
Silk Fibroin ◽  
Delivery System ◽  
Targeted Delivery ◽  
Silk Fibroin Nanoparticles ◽  
Lung Cancer Therapy ◽  
Targeted Delivery System

Bioconjugation of Neutral Protease on Silk Fibroin Nanoparticles and Application in the Controllable Hydrolysis of Sericin

2011 ◽  
Vol 59 (18) ◽  
pp. 10298-10302 ◽  
Author(s):  
Lin Zhu ◽  
Ren-Ping Hu ◽  
Hai-Yan Wang ◽  
Yuan-Jing Wang ◽  
Yu-Qing Zhang
Keyword(s):  
Silk Fibroin ◽  
Neutral Protease ◽  
Silk Fibroin Nanoparticles ◽  
Hydrolysis Of

scholarly journals Silk fibroin nanoparticles dyeing indocyanine green for imaging-guided photo-thermal therapy of glioblastoma

Drug Delivery ◽  
2018 ◽  
Vol 25 (1) ◽  
pp. 364-375 ◽  
Author(s):  
He-Lin Xu ◽  
De-Li ZhuGe ◽  
Pian-Pian Chen ◽  
Meng-Qi Tong ◽  
Meng-Ting Lin ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Silk Fibroin ◽  
Thermal Therapy ◽  
Silk Fibroin Nanoparticles

scholarly journals Improving Anticancer Therapy with Naringenin-Loaded Silk Fibroin Nanoparticles

Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 718 ◽  
Author(s):  
Marta G. Fuster ◽  
Guzmán Carissimi ◽  
Mercedes G. Montalbán ◽  
Gloria Víllora
Keyword(s):  
Silk Fibroin ◽  
Drug Loading ◽  
Drug Release Profile ◽  
Solubility In Water ◽  
Diphenyltetrazolium Bromide ◽  
Wide Range ◽  
Silk Fibroin Nanoparticles ◽  
Ea.Hy926 Cells ◽  
Cytotoxicity Effects

Naringenin (NAR), a flavonoid present in a variety of fruits, vegetables and herbs, exhibits a wide range of pharmacological effects, including anticancer activity. Nevertheless, its application in cancer therapy is limited due to its low bioavailability at the tumour site because of its poor solubility in water and slow dissolution rate. To improve the therapeutic efficacy of NAR, emergent research is looking into using nanocarriers. Silk fibroin (SF), from the Bombyx mori silkworm, is a biocompatible and biodegradable polymer with excellent mechanical properties and an amphiphilic chemistry that make it a promising candidate as a controlled release drug system. The aim of this work is to synthesize naringenin-loaded silk fibroin nanoparticles (NAR-SFNs) by dissolving the SF in the ionic liquid 1-ethyl-3-methylimidazolium acetate, using high-power ultrasounds and rapid desolvation in methanol followed by the adsorption of NAR. The NAR-SFNs were characterized by dynamic light scattering, Fourier transform infrared spectroscopy and thermogravimetric analysis. The drug loading content and encapsulation efficiency were calculated. The drug release profile best fitted a first order equation. The cytotoxicity effects of free NAR, bare silk fibroin nanoparticles (SFNs) and NAR-SFNs were assessed on HeLa and EA.hy926 cells via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results demonstrated the higher in vitro anticancer potential of synthesized NAR-SFNs than that of free NAR in HeLa cancer cells.

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