Silk I structure formation through silk fibroin self-assembly

2012 ◽  
Vol 125 (3) ◽  
pp. 2148-2154 ◽  
Author(s):  
Jinfa Ming ◽  
Baoqi Zuo
Keyword(s):  
Structure Formation ◽  
Silk Fibroin ◽  
Self Assembly

scholarly journals Nanofibrous architecture of silk fibroin scaffolds prepared with a mild self-assembly process

Biomaterials ◽  
2011 ◽  
Vol 32 (4) ◽  
pp. 1059-1067 ◽  
Author(s):  
Qiang Lu ◽  
Xiuli Wang ◽  
Shenzhou Lu ◽  
Mingzhong Li ◽  
David L. Kaplan ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Self Assembly ◽  
Assembly Process

Regenerated Antheraea pernyi Silk Fibroin Nanofiber Film

2012 ◽  
Vol 465 ◽  
pp. 160-164 ◽  
Author(s):  
Li Mao ◽  
Yu Liu ◽  
Xi Long Wu ◽  
Shen Zhou Lu
Keyword(s):  
Surface Tension ◽  
Silk Fibroin ◽  
Self Assembly ◽  
Water Environment ◽  
Antheraea Pernyi ◽  
Thiocyanate Solution ◽  
Nanofiber Film ◽  
20 Nm ◽  
Cell Migration And Proliferation

Regenerated Antheraea Pernyi silk fibroin (ASF) solution was prepared by dissolving Antheraea Pernyi (A. pernyi) silk fiber in lithium thiocyanate solution. Atomic force microscopy (AFM) examination showed that there were many short nanofibers in regenerated ASF solution, which were made up of 10-20 nm nanospheres. In this paper, we discussed the formation mechanism of these nanofibers. The results of the surface tension showed that ASF had surface active and can significantly decline the surface tension of water from 73 mN/m to 51 mN/m. The hydrophobic side chains of ASF molecular would try to break away from water environment and thereby undergo self-assembly into nanospheres. And then, these nanospheres arrange into a short nanofiber. Then, the ASF nanofiber film was prepared by casting regenerated ASF solution blended with glycol. The structure of ASF was β-sheet and the film was water-insoluble. This blend film not only had excellent mechanical properties, but also can be biodegradated by enzyme in vitro. All of this indicated that this new regenerated ASF nanofiber film not only provided excellent biocompatibility, but also had large surface as extracellular matrix (ECM) to cell adhesion. The film made of nanofiber was similar to ECM on the nanoscale, which promoted cell migration and proliferation. In summary, it provided a great potential as a biological material.

Self-Assembly of the Synthetic Polymer (Leu-Glu)n:  An Amyloid-like Structure Formation

Langmuir ◽  
2003 ◽  
Vol 19 (8) ◽  
pp. 3413-3418 ◽  
Author(s):  
Julia P. Moses ◽  
K. S. Satheeshkumar ◽  
J. Murali ◽  
D. Alli ◽  
R. Jayakumar
Keyword(s):  
Structure Formation ◽  
Self Assembly ◽  
Synthetic Polymer

Self-assembly of monolayered lipid membranes for surface-coating of a nanoconfined Bombyx mori silk fibroin film

RSC Advances ◽  
2015 ◽  
Vol 5 (81) ◽  
pp. 65684-65689 ◽  
Author(s):  
Fan Xu ◽  
Meimei Bao ◽  
Longfei Rui ◽  
Jiaojiao Liu ◽  
Jingliang Li ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Silk Fibroin ◽  
Self Assembly ◽  
Lipid Membranes ◽  
Surface Coating ◽  
Lipid Membrane ◽  
Coating Film ◽  
Silk Fibroin Film ◽  
Bombyx Mori Silk ◽  
Self Assembled

A self-assembled lipid membrane provides a smooth, hydrophilic and biocompatible surface coating film for materials.

Patterning: Structure Formation by Dynamic Self-Assembly (Small 4/2012)

Small ◽  
2012 ◽  
Vol 8 (4) ◽  
pp. 487-487 ◽  
Author(s):  
Liqiang Li ◽  
Michael H. Köpf ◽  
Svetlana V. Gurevich ◽  
Rudolf Friedrich ◽  
Lifeng Chi
Keyword(s):  
Structure Formation ◽  
Self Assembly

scholarly journals Free-standing supramolecular hydrogel objects by reaction-diffusion

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Matija Lovrak ◽  
Wouter E. J. Hendriksen ◽  
Chandan Maity ◽  
Serhii Mytnyk ◽  
Volkert van Steijn ◽  
...  
Keyword(s):  
Structure Formation ◽  
Self Assembly ◽  
Reaction Diffusion ◽  
Building Blocks ◽  
Assembly Process ◽  
Supramolecular Hydrogel ◽  
Free Standing ◽  
Spatial Control ◽  
Self Assembling ◽  
Vast Range

Abstract Self-assembly provides access to a variety of molecular materials, yet spatial control over structure formation remains difficult to achieve. Here we show how reaction–diffusion (RD) can be coupled to a molecular self-assembly process to generate macroscopic free-standing objects with control over shape, size, and functionality. In RD, two or more reactants diffuse from different positions to give rise to spatially defined structures on reaction. We demonstrate that RD can be used to locally control formation and self-assembly of hydrazone molecular gelators from their non-assembling precursors, leading to soft, free-standing hydrogel objects with sizes ranging from several hundred micrometres up to centimeters. Different chemical functionalities and gradients can easily be integrated in the hydrogel objects by using different reactants. Our methodology, together with the vast range of organic reactions and self-assembling building blocks, provides a general approach towards the programmed fabrication of soft microscale objects with controlled functionality and shape.

Effect of Macromolecular Crowders on the Self‐Assembly Process of Silk Fibroin

2020 ◽  
Vol 221 (16) ◽  
pp. 2000113 ◽  
Author(s):  
Priyanka Dubey ◽  
Sinchan Seit ◽  
Pramit K. Chowdhury ◽  
Sourabh Ghosh
Keyword(s):  
Silk Fibroin ◽  
Self Assembly ◽  
The Self ◽  
Assembly Process

Silk fabric protection obtained via chemical conjugation of transglutaminase and silk fibroin reinforcement

2019 ◽  
Vol 89 (21-22) ◽  
pp. 4581-4594 ◽  
Author(s):  
Suhua Zhao ◽  
Hongliang Pan ◽  
Yali Liu ◽  
Yirong Zeng ◽  
Hongling Liu ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Secondary Structure ◽  
Silk Fibroin ◽  
Self Assembly ◽  
Sodium Caseinate ◽  
Solution Concentration ◽  
Breaking Stress ◽  
Silk Fabric ◽  
Chemical Conjugation ◽  
Washing Durability

Historic silk fabric is an important part of Chinese precious cultural heritage and its protection has always been a major challenge. This paper proposes a bio-safety method by the chemical conjugation of transglutaminase (TGase or TG) and sodium caseinate (SC), which produced a macromolecular polymer between protein molecules and enhanced silk fabrics. The changes of the mechanical properties of the reinforced silk fabric after washing by 10 cycles were not obvious, indicating good washing durability. After TGase and SC reinforcement, the silk fibroin (SF) solution was sprayed on the surface of silk fabric to improve the mechanical properties, where the secondary structure were formed by the self-assembly of SF to improve the mechanical properties. Therefore, the breaking stress attained the maximum value when the SF solution concentration was 1.0%. Meanwhile, the breaking stress increased by about 20.89% compared with untreated silk fabric. When the artificially alkali aged silk fabric is reinforced, the breaking stress and strain of the reinforced sample increased by 37.77% relative to the alkali aged fabric. The surface morphology and secondary structure transformation of the samples were also analyzed by scanning electron microscopy and Fourier transform infrared spectroscopy, respectively. The results indicated that a significant SF layer was introduced on the surface of the silk fabric and the β-sheet structure increased due to the synergetic role of the macromolecular polymer and SF. Moreover, it is concluded that an increase in temperature and humidity will result in a decrease in the preservation index, which caused the degradation of silk fabric and proved that the preservation time of the reinforced silk fabric in the same environment was longer than that of the unreinforced sample. The biological enzyme chemical conjugation with silk fabric and physical combination of the pure SF solution is expected to be applied to the protection and enhancement of silk cultural relics.

Controllable transition of silk fibroin nanostructures: An insight into in vitro silk self-assembly process

2013 ◽  
Vol 9 (8) ◽  
pp. 7806-7813 ◽  
Author(s):  
S. Bai ◽  
S. Liu ◽  
C. Zhang ◽  
W. Xu ◽  
Q. Lu ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Self Assembly ◽  
Assembly Process ◽  
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