Effects of the process parameters on the initial burst release of poly(lactide-co-glycolide) microspheres containing bovine serum albumin by the double-emulsion solvent evaporation/extraction method

2010 ◽  
Vol 115 (5) ◽  
pp. 2599-2608 ◽  
Author(s):  
Zheng Wang
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Process Parameters ◽  
Bovine Serum ◽  
Extraction Method ◽  
Double Emulsion ◽  
Solvent Evaporation ◽  
Burst Release ◽  
Initial Burst ◽  
Initial Burst Release

Box-Behnken Design Optimized TPGS Coated Bovine Serum Albumin Nanoparticles Loaded with Anastrozole

2021 ◽  
Vol 18 ◽  
Author(s):  
Ashish Kumar ◽  
Ajit Singh ◽  
S.J.S Flora ◽  
Rahul Shukla
Keyword(s):  
Particle Size ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Glycol Succinate ◽  
Burst Release ◽  
Spectroscopic Techniques ◽  
Potential Candidate ◽  
Box Behnken Design ◽  
Thermal Gelation

Purpose: In this study, a novel D-α-tocopheryl polyethylene glycol succinate (TPGS) modified bovine serum albumin (BSA) nanoparticles were developed for delivery of Anastrozole (ANZ) which is optimized by Box-Behnken design (BBD). This TPGS-ANZ-BSA NPs are evaluated for their physicochemical and drug release characteristics. Methods: TPGS-ANZ-BSA NPs were prepared by desolvation thermal gelation method andthe effects of critical process parameter (CPP)which are BSA amount, TPGS concentration and stirring speed on the critical quality attributes (CQA) such as % drug loading (%DL) and particle size were studied using BBD. TPGS-ANZ-BSA NPs were characterized using different spectroscopic techniques including UV-Visible and FTIR is used to confirm the entrapment of ANZ in BSA. DSC and PXRD revealed the amorphization of ANZ in the TPGS-ANZ-BSA NPs after freeze drying. Scanning electron microscopy (SEM) analysis was performed for the surface morphologyanalysesNPs. In vitro release studies were performed at pH 5.5 and pH 7.4 for 48h to mimic tumour microenvironment. Results: The BBD optimized batch showed 107 nm particle size with % DL of 8.5± 0.5 of TPGS-ANZ-BSA NPs. The spectroscopic and thermal characterizations revealed the successful encapsulation of ANZ inside the nanoparticles.The TPGS-ANZ-BSA NPs were found to exhibit burst release at pH 5.5 and sustained release at pH 7.4. The short-term stability of drug-loaded nanoparticles displayed no significant changes in physicochemical properties at room temperature for period of one month. Conclusion: The BBD optimized TPGS-ANZ-BSA nanoparticles showed enhanced physiochemical properties for ANZ and potential candidate for anticancer agent drugs delivery.

Development of double emulsion nanoparticles for the encapsulation of bovine serum albumin

2017 ◽  
Vol 158 ◽  
pp. 190-196 ◽  
Author(s):  
Nelida.Y. Martinez ◽  
Patricia F. Andrade ◽  
Nelson Durán ◽  
Sebastian Cavalitto
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Double Emulsion

scholarly journals Preparation and Characterization of Irinotecan Loaded Cross-Linked Bovine Serum Albumin Beads for Liver Cancer Chemoembolization Therapy

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Jin Yan ◽  
Fei Wang ◽  
Jun Chen ◽  
Tao Liu ◽  
Tao Zhang
Keyword(s):  
Bovine Serum Albumin ◽  
Liver Cancer ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Rabbit Model ◽  
Drug Carriers ◽  
Embolic Agent ◽  
Diffusion Method ◽  
Liver Carcinoma ◽  
Burst Release

In this paper, a novel temporary embolization agent for transarterial chemoembolization of liver cancer was developed and tested. The Irinotecan loaded bovine serum albumin (BSA) beads were tried to be used as embolic agent of liver cancer therapy. BSA beads were prepared by a water-in-oil emulsion solvent diffusion method in soya oil and Span 85 was used as the emulsifier. The obtained BSA beads were able to swell 2.37-fold comparing to dried beads. Depending on the equilibrium swelling process, the Irinotecan was loaded with 9.8% total drug concentration and tested. In vitro drug release studies showed that a burst release of Irinotecan was achieved. Eventually BSA beads were completely degraded in a few weeks. CCK-8 assay demonstrated that BSA beads showed no cytotoxicity against human umbilical vein endothelial cells, and the Irinotecan loaded BSA beads showed comparable cytotoxicity against Hep G2, a human liver carcinoma cell line, as the traditional Irinotecan. In a rabbit model, it was found that BSA beads can successfully be transferred to liver and provide occlusion of small arteries. The present investigation suggested that the BSA beads are promising drug carriers and can potentially be used as temporary embolization agents in interventional oncology.

scholarly journals Effect of Process Parameters on the Initial Burst Release of Protein-Loaded Alginate Nanospheres

2019 ◽  
Vol 10 (3) ◽  
pp. 42 ◽  
Author(s):  
Farhana Yasmin ◽  
Xiongbiao Chen ◽  
Brian Eames
Keyword(s):  
Process Parameters ◽  
Protein Delivery ◽  
Burst Release ◽  
Delivery Device ◽  
Drying Time ◽  
Initial Burst ◽  
Initial Burst Release ◽  
Alginate Concentration ◽  
Model Protein

The controlled release or delivery of proteins encapsulated in micro/nanospheres is an emerging strategy in regenerative medicine. For this, micro/nanospheres made from alginate have drawn considerable attention for the use as a protein delivery device because of their mild fabrication process, inert nature, non-toxicity and biocompatibility. Though promising, one key issue associated with using alginate micro/nanospheres is the burst release of encapsulated protein at the beginning of the release, which may be responsible for exerting toxic side effects and poor efficiency of the delivery device. To address this issue, this study aimed to investigate the effect of process parameters of fabricating protein-loaded alginate nanospheres on the initial burst release. The alginate nanospheres were prepared via a combination of water-in-oil emulsification and the external gelation method and loaded with bovine serum albumin (BSA) as a model protein. The examined process parameters included alginate concentration, ionic cross-linking time and drying time. Once fabricated, the nanospheres were then subjected to the examination of BSA release, as well as the characterization of their morphology, size, and encapsulation efficiency. Our results revealed that by properly adjusting the process parameters, the initial burst release can be reduced by 13%. Taken together, our study demonstrates that regulating process parameters of fabricating alginate nanospheres is a possible means to reduce the initial burst release.

Fabrication of BSA Loaded Poly (Caprolactone) (PCL)/Hydroxyapatite (HA) Composite Microsphere for Tissue Engineering Application

2014 ◽  
Vol 1030-1032 ◽  
pp. 82-85
Author(s):  
Mad Jin Rashid ◽  
Naznin Sultana ◽  
Hamdan Salehhuddin
Keyword(s):  
Tissue Engineering ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Engineering Application ◽  
Double Emulsion ◽  
Average Diameter ◽  
Tissue Engineering Application ◽  
Poly Caprolactone

In this study, PCL microsphere has been synthesised using the double emulsion technique and a freeze dry technique. Bovine Serum Albumin (BSA) was added as a model protein and Hydroxyapatite (HA) to enhance osteoconductivity properties of the microsphere. Poly (caprolactone) (PCL)-based microspheres were synthesized and characterized. Microspheres were produced with 5%, 10%, and 15% (w/v) PCL solution (30mL) by dissolving different amounts of PCL in dichloromethane (DCM). Microspheres produced from 5% (w/v) PCL concentration had an average diameter of 100 – 120 μm. Bovine serum albumin (BSA) (0.04% w/v) and 10% w/w of HA powder were added into the microspheres produced from 5% (w/v) PCL concentration. The microsphere was then characterized using different techniques and in vitro release study of BSA was conducted. Prepared microsphere showed good potential for bone tissue engineering.

scholarly journals FTIR Characterization and Release of Bovine Serum Albumin from Bioactive Glasses

2017 ◽  
Vol 15 (4) ◽  
pp. e347-e355 ◽  
Author(s):  
Wai-Ching Liu ◽  
Blake Ballenger ◽  
Amnah Algarni ◽  
Mariano Velez ◽  
Tien-Min G. Chu
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bioactive Glass ◽  
Bovine Serum ◽  
Drug Carrier ◽  
Helical Structure ◽  
Burst Release ◽  
Chitosan Coating ◽  
Degradation Rates ◽  
Percent Protein

Background Bioactive glass has attracted substantial interest in orthopedics, but it has been less explored as a drug carrier. This study investigated the bovine serum albumin (BSA) release from bioactive 13–93B0 and 13–93B3 glasses. Methods Glass disks (13–93B0 and 13–93B3; n = 5) were loaded with 4 mg of BSA and coated under different chitosan-coating conditions. The amount of BSA released in phosphate-buffered saline (PBS) was evaluated, and a degradation study was performed to find out the weight loss and pH of PBS. Secondary structures of BSA on 13–93B0 were characterized by Fourier transform infrared (FTIR) spectroscopy. Results One hundred percent protein release occurred by 24 hours for all 13–93B3 groups. However, chitosan coating delayed 100% release up to 72 hours in 13–93B0 groups. The 13–93B3 glass showed higher degradation rates than 13–93B0 regardless of chitosan-coating status. Multilayer and sandwich chitosan coatings further delayed BSA release from 13–93B0. FTIR analysis revealed that α-helical structure was the highest among all groups and significantly higher in the 2% sandwich chitosan coating group (32.0% ± 2.1%), compared with uncoated and 4% chitosan groups. Conclusions Chitosan coating can delay the burst release of BSA from 13–93B0 glass and be a potential coating on bioactive glass for drug delivery purposes.

scholarly journals High Potency of SN-38-Loaded Bovine Serum Albumin Nanoparticles Against Triple-Negative Breast Cancer

Pharmaceutics ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 569 ◽  
Author(s):  
Lin ◽  
Chuang ◽  
Cheng ◽  
Lin ◽  
Fang
Keyword(s):  
Breast Cancer ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Triple Negative Breast Cancer ◽  
Bovine Serum ◽  
Triple Negative ◽  
Control Group ◽  
Burst Release ◽  
Albumin Nanoparticles ◽  
Low Efficiency

Triple-negative breast cancer (TNBC) is an aggressive type of breast cancer with a worse prognosis than other types. There are currently no specific approved treatments for TNBC. Albumin is a promising biomimetic material that may be fabricated into nanoparticles to possibly exert passive effects on targeted tumors. Irinotecan has been extensively used in clinical settings, although a high dosage is required due to its low efficiency of conversion into the active metabolite SN-38, also known as 7-ethyl-10-hydroxy-camptothecin. The aim of this work was to optimize SN-38-loaded bovine serum albumin nanoparticles (sBSANPs) and evaluate their potency against TNBC. The sBSANPs were characterized by a small size of about 134–264 nm, a negative charge of −37 to −40 mV, an entrapment efficiency of 59–71%, and a particle yield of 65–86%. The cytotoxicity assays using sBSANPs showed a higher potency specifically against both MDA-MB-468 and MDA-MB-231 cells (ER−, PR−, HER2−) compared to MCF-7 (ER+, PR+, HER2−), and exhibited an extremely low IC50 at the nanomolar levels (2.01–6.82 nM). The release profiles indicated that SN-38 presented an initial burst release within 12 h, and sBSANPs had a slow release pattern. Flow cytometry results showed that the fluorescence intensity of sBSANPs was significantly higher than that of the control group. The confocal images also confirmed that sBSANPs were taken up by MDA-MB-468 cells. Moreover, we found that a larger BSANP size resulted in an increased hemolytic effect. In vivo animal studies demonstrated that loading of SN-38 into bovine serum albumin nanoparticles could minimize the initial concentration without extending the elimination half-life, but significantly minimized the Cmax (p < 0.001) as compared with irinotecan treatment.

A Novel Thermosensitive Composite Fabricated with Au Nanoparticles, Poly(Lactide), and Poly(N-Isopropylacrylamide)

2004 ◽  
Vol 99-100 ◽  
pp. 161-164
Author(s):  
Yun Suk Jo ◽  
Do-Kyung Kim ◽  
Young Keun Jeong ◽  
Kyung Ja Kim ◽  
Mamoun Muhammed
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Au Nanoparticles ◽  
Double Emulsion ◽  
Drug Carriers ◽  
Shell Structures ◽  
Emulsion Method ◽  
Novel Approach

A novel approach to load a hydrophilic bovine serum albumin (BSA) into the drug carriers was proposed in terms of thermosensitive dual-shell structures which were fabricated with poly(N-isopropylacrylamide), poly(lactide) and Au nanoparticles. Spherically well-defined dualshell structures were constructed by a modified-double-emulsion method (MDEM). The lower critical solubility temperature of the structures was shifted to 36.4 °C which was confirmed by UVVis spectroscopy.

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