Preparation and characterization of regeneratedBombyx mori silk fibroin fiber containing recombinant cell-adhesive proteins; Nonwoven fiber and monofilament

2008 ◽  
Vol 109 (5) ◽  
pp. 2956-2963 ◽  
Author(s):  
Zhenghua Zhu ◽  
Kosuke Ohgo ◽  
Reiko Watanabe ◽  
Toshiaki Takezawa ◽  
Tetsuo Asakura
Keyword(s):  
Silk Fibroin ◽  
Silk Fibroin Fiber ◽  
Cell Adhesive ◽  
Recombinant Cell ◽  
Adhesive Proteins ◽  
Nonwoven Fiber

scholarly journals Characterization of water in hydrated Bombyx mori silk fibroin fiber and films by 2H NMR relaxation and 13C solid state NMR

2017 ◽  
Vol 50 ◽  
pp. 322-333 ◽  
Author(s):  
Tetsuo Asakura ◽  
Kotaro Isobe ◽  
Shunsuke Kametani ◽  
Obehi T. Ukpebor ◽  
Moshe C. Silverstein ◽  
...  
Keyword(s):  
Solid State ◽  
Bombyx Mori ◽  
Silk Fibroin ◽  
Solid State Nmr ◽  
Nmr Relaxation ◽  
2H Nmr ◽  
Silk Fibroin Fiber ◽  
Bombyx Mori Silk

Preparation and Characterization of Silk Fibroin/Hydroxyapatite Bilayer Scaffolds

2011 ◽  
Vol 415-417 ◽  
pp. 1810-1815 ◽  
Author(s):  
Jian Bing Liu ◽  
Qiang Tang ◽  
Shen Zhou Lu ◽  
Ceng Zhang ◽  
Ming Zhong Li
Keyword(s):  
Silk Fibroin ◽  
Electron Excitation ◽  
Mouse Bone Marrow ◽  
High Porosity ◽  
X Ray Diffraction ◽  
Tissue Engineering Scaffolds ◽  
Molding Method ◽  
Isostatic Compaction ◽  
Fibroin Solution

When the articular cartilage defect accompanies with the subchondral bone defect, using bilayer scaffolds which can integrate with surrounding host cartilage and bone tissue respectively as the tissue engineering scaffolds will be conducive to the repair of tissue defects. This paper reports a new method for preparing bilayer scaffolds. Firstly, hydroxyapatite (HA)/silk fibroin(SF) composite porous materials which have high porosity were prepared by a isostatic compaction molding method, then it was fully immersed in silk fibroin solution, and finally SF/HA bilayer scaffolds were obtained by freeze-drying. The structure of the bilayer scaffolds were investigated through scanning electron microscopy, X-ray diffraction, Fourier transform-infrared spectroscopy, electron excitation spectroscopy and electron microprobe. The results indicated that the upper layer of SF/HA bilayer scaffolds is porous SF component, the under layer is the porous HA/SF composite component and the interface of the two layer is closely connected. Furthermore, mesenchymal stem cells from mouse bone marrow were seeded into the bilayer scaffolds and the results showed that the cells had a well adhesion and growth after culturing for 3 days.

Preparation and characterization of silk fibroin/strontium carbonate film through rapid formation

2017 ◽  
Vol 189 ◽  
pp. 46-49 ◽  
Author(s):  
Min Liu ◽  
Feng Zhang ◽  
Peng Wang ◽  
Jinfa Ming ◽  
Baoqi Zuo
Keyword(s):  
Silk Fibroin ◽  
Strontium Carbonate ◽  
Rapid Formation ◽  
Carbonate Film

Recombinant silk fibroin incorporated cell-adhesive sequences produced by transgenic silkworm as a possible candidate for use in vascular graft

2014 ◽  
Vol 2 (42) ◽  
pp. 7375-7383 ◽  
Author(s):  
Tetsuo Asakura ◽  
Makoto Isozaki ◽  
Toshiki Saotome ◽  
Ken-ichiro Tatematsu ◽  
Hideki Sezutsu ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Vascular Graft ◽  
Transgenic Silkworm ◽  
Cell Adhesive ◽  
Silk Fibroins

Transgenic silk fibroins that incorporated the laminin sequence were prepared. The adhesive activities tend to increase in the TG silk fibroins relative to WT silk fibroins.

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