Investigation of amino acid composition in the crystalline region of silk fibroin

1985 ◽  
Vol 30 (1) ◽  
pp. 221-225 ◽  
Author(s):  
G. S. Nadiger ◽  
N. V. Bhat ◽  
M. R. Padhye
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Silk Fibroin ◽  
Crystalline Region

Three-Layered Sericins around the Silk Fibroin Fiber from Bombyx mori Cocoon and their Amino Acid Composition

2011 ◽  
Vol 175-176 ◽  
pp. 158-163 ◽  
Author(s):  
Yuan Jing Wang ◽  
Yu Qing Zhag
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Silk Fibroin ◽  
Reference Value ◽  
Solubility In Water ◽  
Silk Fibroin Fiber ◽  
Different Temperatures ◽  
Hydrophobic Amino Acids

The sericin coated around silk fibroin fiber is a natural protein-based polymer and presents a layered structure. According to the solubility in water under different temperatures and pressures, the sericin is divided into three parts of outer, middle and inner sericin layers accounting for 15%, 10.5% and 4.5% to the total silk protein (included sericin layers and fibroin fiber) in terms of the mass respectively. The partition within the sericin layer presents the relative proportions of 50%, 35% and 15% from the outer to inner layer. Furthermore, the differences of three layers in the amino acid composition are very significant. The non-polar amino acids in the outer, middle and inner sericins are gradually increased, in particular for alanine, which are accounted for 5.20, 6.13 and 11.58 mol% respectively; while its content jumps to 33.38 mol% in the silk fibroin fiber. Nevertheless, the polar amino acids, especially the neutral ones, are gradually decreased accounting for 39.34, 38.62 and 23.82 mol% respectively. Concentration of Serine drops most prominently, i.e. 28.00, 25.57 and 13.32 mol% respectively; while its content goes to 7.65 mol% in the silk fibroin fiber. On the other hand, the hydrophobic amino acids gradually increase and the hydrophilic amino acids go to oppsite way. These results indicate that the amino acid compositions of the outer and middle sericins are similar to each other, but those of the inner sericin are different from the others. The closer the sericin layer is to the silk fibroin fiber, the closer its amino acid composition is to that of the fibroin fiber. These obtained results are of important reference value for processing of silk sericin peptides and their applications in cosmetics, cell culture, healthy food and other areas.

Research Progress in Sequences Comparison and Crystal Structure of Silk Fibroin

2013 ◽  
Vol 664 ◽  
pp. 443-448 ◽  
Author(s):  
Bao Shan Yang ◽  
Jun Li ◽  
Hui Wang
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Silk Fibroin ◽  
Molecular Conformation ◽  
Research Progress ◽  
Sequence Characterization ◽  
Fibrous Protein ◽  
Unique Amino Acid ◽  
Sequence Characteristics

Silk fibroin is native fibrous protein extracted from silk. It has good mechanical properties because of its unique amino acid composition, complex of sequence characterization and the transform of crystalline structure under different condition. The characteristics of silk fibroin makes it better biological biocompatibility and biodegradability and have been used widely as biomaterials in the field of tissue engineering. Based on the introduction of its properties, the researches on the amino acid composition, sequence characteristics, molecular conformation, and its performance were reviewed in this paper.

The Amino Acid Composition of Bombyx mori Silk Fibroin and of Tussah Silk Fibroin

1955 ◽  
Vol 77 (14) ◽  
pp. 3908-3913 ◽  
Author(s):  
W. A. Schroeder ◽  
Lois M. Kay ◽  
Barry Lewis ◽  
Nancy Munger
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Bombyx Mori ◽  
Acid Composition ◽  
Silk Fibroin ◽  
Bombyx Mori Silk ◽  
Tussah Silk Fibroin

Effect of maternal diet on the amino acid composition of human uterine fluid

2014 ◽  
Author(s):  
Alexandra Jayne Kermack ◽  
Ying Cheong ◽  
Nick Brook ◽  
Nick Macklon ◽  
Franchesca D Houghton
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Maternal Diet ◽  
Uterine Fluid
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