The effects of flow rate and column combination on the separation efficiency in multicolumn gel permeation chromatography

1977 ◽  
Vol 21 (9) ◽  
pp. 2439-2451 ◽  
Author(s):  
M. R. Ambler ◽  
L. J. Fetters ◽  
Y. Kesten
Keyword(s):  
Flow Rate ◽  
Separation Efficiency ◽  
Gel Permeation Chromatography ◽  
Gel Permeation

Gel permeation chromatography: The effect of flow rate on efficiency. II

1983 ◽  
Vol 28 (2) ◽  
pp. 473-483 ◽  
Author(s):  
Jau-Yi Chuang ◽  
Julian F. Johnson ◽  
Anthony R. Cooper
Keyword(s):  
Flow Rate ◽  
Gel Permeation Chromatography ◽  
Gel Permeation

scholarly journals Effects of the electrosprayed polycaprolactone microparticles morphology on the polycaprolactone degradation

2019 ◽  
Vol 3 (2) ◽  
pp. 65-73
Author(s):  
Nguyen-Vu Viet Linh ◽  
Nguyen Quoc Viet ◽  
Huynh Dai Phu
Keyword(s):  
Electron Microscopy ◽  
Flow Rate ◽  
Protein Delivery ◽  
Gel Permeation Chromatography ◽  
Processing Parameters ◽  
The Body ◽  
Processing Parameter ◽  
Gel Permeation ◽  
Polymeric Microparticles ◽  
Scanning Electron

The polycaprolactone (PCL) microparticles fabricated by electrospray technique have been studied and applied in drug and protein delivery system. The degradation of PCL and the release of drug/protein from the polymeric microparticles (MPs) were desired to happen simultaneously. When the new dosage was administrated, the PCL MPs were degraded and eliminated out of the body. This research indicated that the degradation of PCL was influenced by the various morphology of electrosprayed microparticles. The different sizes of 11.8 μm and 5.17 μm and the various shapes of the PCL MPs such as hollow, porous and wrinkle particles and spheres were investigated the PCL degradation in the PBS solution, at pH 7.4. The morphology of PCL MPs was designed by controlling the polymer solution and the electrosprayed processing parameters such as the flow rate and collecting distance. Scanning electron microscopy and gel permeation chromatography were order to determine the change of the morphology and number molecule weight (Mn) of PCL MPs. The porous, distorted and smaller particles reduced the Mn faster than the microspheres because of the larger surface area of MPs contacted with PBS solution. After 77 days, PCL MPs which were fabricated by the processing parameter, including 2.5% PCL in DCM, flow rate of 0.8 mL/h, voltage of 18 kV, collecting distance of 25 cm, reduced 49.96% molecular weight (decreasing from Mn= 80,438 g/mol to 40,225 g/mol).  

Reliability of the linear effective calibration method in gel permeation chromatography

1984 ◽  
Vol 49 (10) ◽  
pp. 2197-2204 ◽  
Author(s):  
Miroslav Kubín
Keyword(s):  
Separation Efficiency ◽  
Gel Permeation Chromatography ◽  
Calibration Method ◽  
Calibration Standard ◽  
Linear Calibration ◽  
Average Molar Mass ◽  
Gel Permeation ◽  
Calibration Dependence ◽  
The Given ◽  
Calculated Average

The validity of the effective linear calibration (ELC) method in gel permeation chromatography of polymers has been checked by means of computer generated chromatograms. The ratio Mw/Mn calculated using ELC form an uncorrected chromatogram is always smaller than that determined by means of the true calibration dependence, but the extent of this correction for longitudinal spreading depends on the polydispersity of both the original calibration standard and the analyzed polymer, and also on the separation efficiency of the given column. If the respective molecular weight averages of the analyzed sample and of the calibration standard differ considerably, the effective linear calibration method leads to serious systematic errors in the calculated average molar mass values.

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