Preparation and characterization of an enzyme electrode based on cholesterol esterase and cholesterol oxidase immobilized onto conducting polypyrrole films

2004 ◽  
Vol 91 (6) ◽  
pp. 3769-3773 ◽  
Author(s):  
Suman Singh ◽  
Asha Chaubey ◽  
B. D. Malhotra
Keyword(s):  
Cholesterol Oxidase ◽  
Enzyme Electrode ◽  
Cholesterol Esterase ◽  
Polypyrrole Films ◽  
Conducting Polypyrrole

Enzyme Electrode with Collagen-Immobilized Cholesterol Oxidase for the Microdetermination of Free Cholesterol

1979 ◽  
Vol 12 (14) ◽  
pp. 1477-1488 ◽  
Author(s):  
Catherine Bertrand ◽  
Pierre R. Coulet ◽  
Danièle C. Gautheron
Keyword(s):  
Free Cholesterol ◽  
Cholesterol Oxidase ◽  
Enzyme Electrode

scholarly journals A simple method for the determination of the cholesterol esterase activity.

2013 ◽  
Vol 60 (3) ◽  
Author(s):  
Agnieszka Ewa Stępień ◽  
Mykhailo Gonchar
Keyword(s):  
Human Serum ◽  
Esterase Activity ◽  
Cholesterol Oxidase ◽  
Cholesterol Esterase ◽  
Cholesterol Esters ◽  
Simple Method ◽  
Bacterial Enzyme ◽  
Low Costs ◽  
Hydrolysis Of

The proposed method determines the activity of cholesterol esterase (CEH) and takes advantage of its ability to catalyze the hydrolysis of cholesterol esters naturally present in human serum. The assay is based on Allain's method of spectrophotometric determination of cholesterol by means of cholesterol oxidase, peroxidase, but using 3,5-dichloro-dihydroxybenzenesulfonic acid (DHBS) as phenolic chromogen and human serum as a source of substrate for the CEH as a novelty. Furthermore, it is characterized by low costs and high precision. It can be employed to control the activity of CE preparations used for the preparation of enzymatic kits for the determination of cholesterol or for screening of potential bacterial enzyme producers.

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