Monitoring the grafting of epoxidized natural rubber by size-exclusion chromatography coupled to FTIR spectroscopy

2003 ◽  
Vol 88 (10) ◽  
pp. 2539-2549 ◽  
Author(s):  
Andr� J. P. Van Zyl ◽  
Sven M. Graef ◽  
Ronald D. Sanderson ◽  
Bert Klumperman ◽  
Harald Pasch
Keyword(s):  
Natural Rubber ◽  
Ftir Spectroscopy ◽  
Size Exclusion Chromatography ◽  
Size Exclusion ◽  
Epoxidized Natural Rubber ◽  
Exclusion Chromatography

Characterization of natural rubber using size-exclusion chromatography with online multi-angle light scattering

2008 ◽  
Vol 1213 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Chandy Kim ◽  
Marie-Hélène Morel ◽  
Jérôme Sainte Beuve ◽  
Stéphane Guilbert ◽  
André Collet ◽  
...  
Keyword(s):  
Light Scattering ◽  
Natural Rubber ◽  
Size Exclusion Chromatography ◽  
Size Exclusion ◽  
Exclusion Chromatography

UNRAVELING THE MYSTERY OF NATURAL RUBBER BIOSYNTHESIS. PART II: COMPOSITION AND GROWTH OF IN VITRO NATURAL RUBBER USING HIGH-RESOLUTION SIZE EXCLUSION CHROMATOGRAPHY

2014 ◽  
Vol 87 (3) ◽  
pp. 451-458 ◽  
Author(s):  
Cheng Ching K. Chiang ◽  
Balaka Barkakaty ◽  
Judit E. Puskas ◽  
Wenshuang Xie ◽  
Katrina Cornish ◽  
...  
Keyword(s):  
High Resolution ◽  
Natural Rubber ◽  
Size Exclusion Chromatography ◽  
Rubber Tree ◽  
Size Exclusion ◽  
Common Source ◽  
Rubber Biosynthesis ◽  
Flight Mass Spectrometry ◽  
Exclusion Chromatography

ABSTRACT The superior properties of natural rubber (cis-1,4-polyisoprene [NR]) are a function of its structure and composition, properties that still remain a mystery and that are irreplaceable by any synthetic rubber. NR from guayule (Parthenium argentatum) has been gaining special interest for its hypoallergenic properties while maintaining superior mechanical properties that are commonly associated with the Brazilian rubber tree (Hevea brasiliensis), the most common source of NR. Techniques exist to isolate washed rubber particles (WRPs) that contain enzymatically active rubber transferase, to study NR biosynthesis, and previous work on the in vitro NR growth in Hevea has demonstrated the presence of around 50 wt% of a low molecular weight ([MW], Mn <10 000 g/mol) fraction. Structural and compositional analyses of this low MW fraction in Hevea are challenging due to the high protein content. We discuss the analysis and composition of guayule latex and WRPs using high-resolution Size Exclusion Chromatography. We also discuss the composition of the soluble fraction of inactive guayule latex using matrix-assisted laser desorption ionization/time of flight mass spectrometry.

UNRAVELING THE MYSTERY OF NATURAL RUBBER BIOSYNTHESIS. PART I: INVESTIGATION OF THE COMPOSITION AND GROWTH OF IN VITRO NATURAL RUBBER USING HIGH RESOLUTION SIZE EXCLUSION CHROMATOGRAPHY

2011 ◽  
Vol 84 (2) ◽  
pp. 166-177 ◽  
Author(s):  
Chengching K. Chiang ◽  
Wenshuang Xie ◽  
Colleen McMahan ◽  
Judit E. Puskas
Keyword(s):  
High Resolution ◽  
Natural Rubber ◽  
Size Exclusion Chromatography ◽  
Weight Fraction ◽  
Size Exclusion ◽  
Gravimetric Analysis ◽  
In Vitro Synthesis ◽  
Rubber Biosynthesis ◽  
Exclusion Chromatography

Abstract Monitoring the growth of in vitro natural rubber was accomplished by high resolution size exclusion chromatography, SEC. Washed rubber particles isolated from H. brasiliensis latex, containing the rubber transferase enzyme, were used to catalyze the polymerization of synthetic isopentenyl pyrophosphate monomer in the presence of farnesyl pyrophosphate initiator. The high-resolution SEC was able to detect the formation of new rubber. Changes in the low molecular weight fraction were also detected. Gravimetric analysis revealed ∼30% mass gain after the in vitro synthesis. The overall gel content was found to be reduced, which further supported the formation of new rubber. This is the first report that utilizes high-resolution SEC to monitor the in vitro NR growth without the use of radiolabeling.

EFFECT OF MASTICATION ON THE STRUCTURE OF MICROGEL PRESENT IN NATURAL RUBBER

2017 ◽  
Vol 90 (3) ◽  
pp. 445-454 ◽  
Author(s):  
Sébastien Rolere ◽  
Florian Deme ◽  
Jérôme Sainte-Beuve ◽  
Frédéric Bonfils
Keyword(s):  
Light Scattering ◽  
Natural Rubber ◽  
Size Exclusion Chromatography ◽  
Size Exclusion ◽  
Radius Of Gyration ◽  
Random Coils ◽  
Exclusion Chromatography ◽  
Aggregate Fractions

ABSTRACT Mechanical or thermal mastication experiments were performed on three commercial natural rubber (NR) samples of TSR10 grade made from latex of three different clones (GT1, PB235, and RRIM600). The mesostructure (different gel or aggregate fractions, structure of random coils of cis-1,4-polyisoprene) of all the NR samples was fully characterized by size exclusion chromatography coupled with multiangle light scattering (SEC-MALS), using pretreated SEC columns. This method was used to quantify and investigate the structure of the little-studied smaller microaggregates, constituting the microgel fraction smaller than 1 μm (Microgel<1μ) of NR. The three unmasticated NR samples showed no difference in terms of microaggregate structure. Conversely, microaggregates appeared denser after mastication. This phenomenon was found to depend on the mastication conditions, as mechanically masticated NR samples had smaller (lower radius of gyration) and more compact microaggregates than thermally masticated samples. Macrogel also behaved differently depending on the mastication conditions. Mechanical mastication conditions allowed a higher degradation of the macrogel compared with thermal mastication conditions.

29Si NMR, Sec and Ftir Studies of The Hydrolysis and Condensation of Si(OC2H5 )4 and Si2O(OC2H5)6

1986 ◽  
Vol 73 ◽  
Author(s):  
Chia-Cheng Lin ◽  
John D. Basil
Keyword(s):  
High Resolution ◽  
Ftir Spectroscopy ◽  
Size Exclusion Chromatography ◽  
Reaction Scheme ◽  
Size Exclusion ◽  
29Si Nmr ◽  
Reaction Products ◽  
Ftir Studies ◽  
Hydrolysis And Condensation ◽  
Exclusion Chromatography

ABSTRACTThe hydrolysis and initial condensation reactions of Si(OC2H5)4 and low order polyethoxysiloxanes have been studied with high resolution 29Si NMR and size exclusion chromatography/FTIR spectroscopy. The effects of various parameters such as H2O/TEOS mole ratio, catalyst, pH, solvent, temperature and aging have been observed in the different nature of the reaction products. The products include partially hydrolyzed and unhydrolyzed Si(OC2H5)4, Si2O(CH2H5)6, and Si3O2(OC2H5)8. The rate of formation and concentration of each product obtained under different conditions provides information about the reactivity of various silanol-containing species and the factors that affect the overall reaction scheme.

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