scholarly journals Phase-separated polymer blends: Complementary studies between scanning electron microscopy, epifluorescence microscopy, and fluorescence microspectroscopy

2001 ◽  
Vol 80 (7) ◽  
pp. 949-955 ◽  
Author(s):  
E. G. Granados ◽  
J. González-Benito ◽  
J. Baselga ◽  
D. Dibbern-Brunelli ◽  
T. D. Z. Atvars ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Polymer Blends ◽  
Epifluorescence Microscopy ◽  
Scanning Electron

Cryo-Scanning Electron Microscopy: A New Tool for Interpretation of Fracture Studies in Bitumen/Polymer Blends

2002 ◽  
Vol 16 (1) ◽  
pp. 143-147 ◽  
Author(s):  
L. Champion-Lapalu ◽  
A. Wilson ◽  
G. Fuchs ◽  
D. Martin ◽  
J.-P. Planche
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Polymer Blends ◽  
Cryo Scanning Electron Microscopy ◽  
Scanning Electron

scholarly journals Comparison of techniques for counting prokaryotes in marine planktonic and biofilm samples

2021 ◽  
Vol 85 (3) ◽  
pp. 211-220
Author(s):  
Vanessa Ochi Agostini ◽  
Letícia Terres Rodrigues ◽  
Alexandre José Macedo ◽  
Erik Muxagata
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Damage ◽  
High Variability ◽  
Epifluorescence Microscopy ◽  
Free Living ◽  
Marine Biofilm ◽  
Comparison Of Techniques ◽  
Green Fluorescent ◽  
Scanning Electron

Though a large number of techniques are available for the study of aquatic bacteria, the aim of this study was to establish a technique for analysing free-living and biofilm prokaryotic cells through laboratory assays. In particular, we wished to analyse the efficiency of ultrasound to detach and disrupt biofilm, to obtain an efficient stain treatment for quantifying free-living and biofilm prokaryotes in flow cytometry (FC), and to compare epifluorescence microscopy (EFM), scanning electron microscopy (SEM) and FC for quantifying free-living and biofilm prokaryotes#. Marine-grade plywood substrates were immersed in natural marine water that was conditioned for 12 days. At 6 and 12 days, water aliquots and substrates were removed to estimate free-living and biofilm prokaryote density. Ultrasound efficiently removed marine biofilm from substrates (up to 94%) without cell damage. FC analysis (unstained) reliably quantified marine plankton and young or mature biofilm prokaryotes compared with other staining (acridine orange, 4′,6-diamidino-2-phenylindole, propidium iodide and green fluorescent nucleic acid), EFM or SEM techniques. FC and SEM achieved similar results, while a high variability was observed in the EFM technique. FC was faster and more precise than SEM because the count is not dependent on the observer.

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