Rubber biosynthesis by aHevea latex bottom-fraction membrane

2002 ◽  
Vol 87 (1) ◽  
pp. 90-96 ◽  
Author(s):  
Dhirayos Wititsuwannakul ◽  
Atiya Rattanapittayaporn ◽  
Rapepun Wititsuwannakul
Keyword(s):  
Rubber Biosynthesis ◽  
Bottom Fraction

Initiation of rubber biosynthesis: In vitro comparisons of benzophenone-modified diphosphate analogues in three rubber-producing species

2008 ◽  
Vol 69 (14) ◽  
pp. 2539-2545 ◽  
Author(s):  
Wenshuang Xie ◽  
Colleen M. McMahan ◽  
Amanda J. DeGraw ◽  
Mark D. Distefano ◽  
Katrina Cornish ◽  
...  
Keyword(s):  
Rubber Biosynthesis

scholarly journals Regulatory Potential of bHLH-Type Transcription Factors on the Road to Rubber Biosynthesis in Hevea brasiliensis

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 674
Author(s):  
Tomoko Yamaguchi ◽  
Yukio Kurihara ◽  
Yuko Makita ◽  
Emiko Okubo-Kurihara ◽  
Ami Kageyama ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Natural Rubber ◽  
Hevea Brasiliensis ◽  
Time Course ◽  
Molecular Mechanisms ◽  
Cultured Cells ◽  
Early Stage ◽  
Rubber Biosynthesis ◽  
On The Road

Natural rubber is the main component of latex obtained from laticifer cells of Hevea brasiliensis. For improving rubber yield, it is essential to understand the genetic molecular mechanisms responsible for laticifer differentiation and rubber biosynthesis. Jasmonate enhances both secondary laticifer differentiation and rubber biosynthesis. Here, we carried out time-course RNA-seq analysis in suspension-cultured cells treated with methyljasmonic acid (MeJA) to characterize the gene expression profile. Gene Ontology (GO) analysis showed that the term “cell differentiation” was enriched in upregulated genes at 24 h after treatment, but inversely, the term was enriched in downregulated genes at 5 days, indicating that MeJA could induce cell differentiation at an early stage of the response. Jasmonate signaling is activated by MYC2, a basic helix–loop–helix (bHLH)-type transcription factor (TF). The aim of this work was to find any links between transcriptomic changes after MeJA application and regulation by TFs. Using an in vitro binding assay, we traced candidate genes throughout the whole genome that were targeted by four bHLH TFs: Hb_MYC2-1, Hb_MYC2-2, Hb_bHLH1, and Hb_bHLH2. The latter two are highly expressed in laticifer cells. Their physical binding sites were found in the promoter regions of a variety of other TF genes, which are differentially expressed upon MeJA exposure, and rubber biogenesis-related genes including SRPP1 and REF3. These studies suggest the possibilities that Hb_MYC2-1 and Hb_MYC2-2 regulate cell differentiation and that Hb_bHLH1 and Hb_bHLH2 promote rubber biosynthesis. We expect that our findings will help to increase natural rubber yield through genetic control in the future.

scholarly journals Integration of Transcriptomes, Small RNAs, and Degradome Sequencing to Identify Putative miRNAs and their Targets Related to Eu-Rubber Biosynthesis in Eucommia ulmoides

Genes ◽  
2019 ◽  
Vol 10 (8) ◽  
pp. 623
Author(s):  
Jing Ye ◽  
Wenjing Han ◽  
Ruisheng Fan ◽  
Minhao Liu ◽  
Long Li ◽  
...  
Keyword(s):  
Regulatory Network ◽  
Small Rnas ◽  
High Throughput Sequencing ◽  
Expression Profiles ◽  
Vital Role ◽  
Eucommia Ulmoides ◽  
Messenger Rnas ◽  
Rubber Biosynthesis ◽  
Degradome Sequencing ◽  
The Eu

Eucommia ulmoides has attracted much attention as a valuable natural rubber (Eu-rubber) production tree. As a strategic material, Eu-rubber plays a vital role in general and defence industries. However, the study of Eu-rubber biosynthesis at a molecular level is scarce, and the regulatory network between microRNAs (miRNAs) and messenger RNAs (mRNAs) in Eu-rubber biosynthesis has not been assessed. In this study, we comprehensively analyzed the transcriptomes, small RNAs (sRNAs) and degradome to reveal the regulatory network of Eu-rubber biosynthesis in E. ulmoides. A total of 82,065 unigenes and 221 miRNAs were identified using high-throughput sequencing; 20,815 targets were predicted using psRNATarget software. Of these targets, 779 miRNA-target pairs were identified via degradome sequencing. Thirty-one miRNAs were differentially expressed; 22 targets of 34 miRNAs were annotated in the terpenoid backbone biosynthesis pathway (ko00900) based on the Kyoto Encyclopedia of Genes and Genomes (KEGG). These miRNAs were putatively related to Eu-rubber biosynthesis. A regulatory network was constructed according to the expression profiles of miRNAs and their targets. These results provide a comprehensive analysis of transcriptomics, sRNAs and degradome to reveal the Eu-rubber accumulation, and provide new insights into genetic engineering techniques which may improve the content of Eu-rubber in E. ulmoides.

RUBBER CITY GIRL: THE PATH TO THE GOODYEAR MEDAL

2018 ◽  
Vol 91 (1) ◽  
pp. 1-26 ◽  
Author(s):  
Judit E. Puskas
Keyword(s):  
New Technologies ◽  
Breast Implants ◽  
Thermoplastic Elastomers ◽  
Drug Eluting Stent ◽  
Special Method ◽  
Rubber Biosynthesis ◽  
On Line ◽  
Line Process ◽  
Natural Rubber Biosynthesis ◽  
Halogen Free

ABSTRACT An overview of my 40-year career will be provided, spanning both industry and academe, and two continents. During my industrial years at LANXESS (formerly the Rubber Division of Bayer), I solved long-standing (10-yr) major manufacturing problems related to Taktene-55 and developed on-line and off-line process control tools that are still in operation. I also developed new technologies (bimodal butyl, one-step halobutyl, branched butyl, liquid carbon dioxide process) that resulted in patents. After transferring to academe, I continued the development of new polyisobutylene-based materials. I have held the Bayer (LANXESS) Industrial Research Chair for 12 yr, working closely with the rubber industry. My most important accomplishments include developing advanced elastomers and thermoplastic elastomers for health care, enzyme-catalyzed polymer functionalization, a “green” synthesis of disulfide polymers and gels, and research into natural rubber biosynthesis. Poly(styrene-isobutylene-polystyrene) is used in a Food and Drug Administration–approved drug-eluting stent, implanted in more than six million patients, saving lives. The recently patented poly(alloocimene-isobutylene-alloocimene) is also a potential biomaterial and also a potential halogen-free halobutyl rubber. I will also discuss my adventure of a field experiment at a Brazilian Hevea plantation to verify our laboratory discovery that the rubber content of Hevea latex can be increased by 20–50% using a special method of tapping. My goal now is creating safer breast implants with cancer-fighting and healing properties. I am proud that the Rubber World trade journal listed me among the 125 inventors that influenced rubber technology in a profound way. I thank my family, Professor Joseph P. Kennedy, and Dr. Adel Halasa for their mentorship and support.

scholarly journals Rubber Biosynthesis in Latex of Hevea brasiliensis.

1957 ◽  
Vol 32 (6) ◽  
pp. 643-648 ◽  
Author(s):  
Robert S. Bandurski ◽  
Howard J. Teas
Keyword(s):  
Hevea Brasiliensis ◽  
Rubber Biosynthesis
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