scholarly journals Effect of different cell disruption methods on lipid yield of Schizochytrium sp.

2021 ◽  
Author(s):  
Mustafa Hac İsa ◽  
Cansu Metin ◽  
Ertan Ercan ◽  
Yunus Alparslan
Keyword(s):  
Cell Disruption ◽  
Lipid Yield

scholarly journals Identification of microalgae from waste stabilization ponds and evaluation of electroflotation by alternate current for simultaneous biomass separation and cell disruption

2019 ◽  
Vol 24 (1) ◽  
pp. 177-186 ◽  
Author(s):  
Anna Patricya Florentino ◽  
Mayara Carantino Costa ◽  
José Gilmar Silva Nascimento ◽  
Eliezer Fares Abdala-Neto ◽  
Cesar Rossas Mota ◽  
...  
Keyword(s):  
Low Cost ◽  
Domestic Wastewater ◽  
Alternate Current ◽  
Cell Disruption ◽  
Waste Stabilization Ponds ◽  
High Frequencies ◽  
Lipid Yield ◽  
Algal Diversity ◽  
Microalgae Harvesting ◽  
Waste Stabilization

ABSTRACT This work aimed to investigate algal diversity at the genus level in stabilization pond systems treating domestic wastewater and to evaluate the feasibility of an electroflotation by alternate current (EFAC) system for simultaneous microalgae separation and cell disruption. Evaluation of algal diversity showed that the genera Euglena and Chlorella were present in relatively high frequencies in five of the six effluents analyzed. The use of EFAC on an effluent that presented bloom of Chlorella achieved turbidity and chlorophyll-a removal efficiencies higher than 70 and 90%, respectively, after 70 minutes of operation. Total lipid yield for the Chlorella-rich biomass was 21.4±2.02%. Such high biomass lipid content demonstrates the potential for obtaining lipid-based biofuels from wastes. The current paper describes the first attempt, with promising results, at using electroflotation by alternate current for low cost, simultaneous microalgae harvesting and disruption.

scholarly journals Effect of different cell disruption methods on lipid yield of Schizochytrium sp.

2021 ◽  
Author(s):  
Mustafa Hac İsa ◽  
Cansu Metin ◽  
Ertan Ercan ◽  
Yunus Alparslan
Keyword(s):  
Fatty Acids ◽  
Osmotic Shock ◽  
Lipid Extraction ◽  
Extraction Methods ◽  
Cell Disruption ◽  
Health Indices ◽  
Lipid Yield ◽  
Atherogenicity Index ◽  
Total Fatty Acids ◽  
Enzyme Application

In this study, it was investigated to increase the lipid yield of the microalgae Schizochytrium sp., by applying different cell disruption methods. Therefore, acid treatment with HCl, osmotic shock, enzyme applications and ultrasonic homogenizer were tried in this algae species combined with the Bligh and Dyer and Soxhlet methods as an alternative to classical lipid extraction methods. As a result of the study, the highest lipid value (21.72 ± 0.74%) was obtained in enzyme application with Bligh and Dyer method (BDE). The cell disruption processes increased the lipid yield compared to the control groups. The highest PUFA DHA was found in the range of 4.58 ± 2.44-19.25 ± 0.09%, and the highest value was observed in the BDE group. Highest SFA was palmitic acid. Effective results were observed in the Bligh and Dyer applied groups in terms of both total lipid and total fatty acids. In cell disruption methods, particularly in enzyme and HCl extraction, good results were obtained in terms of fatty acids. The highest total fatty acids and the highest lipid content were detected in the Bligh and Dyer enzyme (BDE). Enzyme applications are also advantageous because of being environmentally friendly. Lipid health indices such as n-6/n-3, PUFA/SFA, Atherogenicity index (AI), Thrombogenicity index (TI) and hypocholesterolemic/hypercholesterolemic ratios (HH) were almost favorable. With this study, an appropriate lipid extraction methods were determined to provide an economical and environmental friendly suggestion for future studies to be used in areas such as food, feed and cosmetics.

Optimization of yeast cell disruption with a newly designed bead mill

1991 ◽  
Vol 13 (8) ◽  
pp. 665-671 ◽  
Author(s):  
D. van Gaver ◽  
A. Huyghebaert
Keyword(s):  
Yeast Cell ◽  
Cell Disruption ◽  
Bead Mill

Integrated biomolecular and bioprocess engineering strategies for enhancing the lipid yield from microalgae

2021 ◽  
Vol 148 ◽  
pp. 111270
Author(s):  
Bunushree Behera ◽  
Yuwalee Unpaprom ◽  
Rameshprabu Ramaraj ◽  
Gaanty Pragas Maniam ◽  
Natanamurugaraj Govindan ◽  
...  
Keyword(s):  
Bioprocess Engineering ◽  
Lipid Yield

scholarly journals Extraction of microalgal starch and pigments by using different cell disruption methods and aqueous two‐phase system

2021 ◽  
Author(s):  
Fabrizio Di Caprio ◽  
Rachele Chelucci ◽  
Iolanda Francolini ◽  
Pietro Altimari ◽  
Francesca Pagnanelli
Keyword(s):  
Cell Disruption ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

scholarly journals Comparison of Different Methods for Extracting the Astaxanthin from Haematococcus pluvialis: Chemical Composition and Biological Activity

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3569
Author(s):  
Yicheng Tan ◽  
Zhang Ye ◽  
Mansheng Wang ◽  
Muhammad Faisal Manzoor ◽  
Rana Muhammad Aadil ◽  
...  
Keyword(s):  
High Pressure ◽  
Biological Activity ◽  
Chemical Composition ◽  
Haematococcus Pluvialis ◽  
Activity Analysis ◽  
Cell Disruption ◽  
Dependent Manner ◽  
Dpph Activity ◽  
The Impact ◽  
Dose Dependent

In this study, the impact of different cell disruption techniques (high-pressure micro fluidization (HPMF), ionic liquids (ILs), multi-enzyme (ME), and hydrochloric acid (HCl)) on the chemical composition and biological activity of astaxanthin (AST) obtained from Haematococcus pluvialis was investigated. Results indicated that all cell disruption techniques had a significant effect on AST composition, which were confirmed by TLC and UPC2 analysis. AST recovery from HCl (HCl-AST) and ILs (ILs-AST) cell disruption techniques was dominant by free and monoesters AST, while AST recovery from HPMF (HPMF-AST) and ME (ME-AST) cell disruption techniques was composed of monoesters, diesters, and free AST. Further biological activity analysis displayed that HCl-AST showed the highest ABTS and DPPH activity, while ILs-AST showed better results against the ORAC assay. Additionally, ILs-AST exhibits a stronger anti-proliferation of HepG2 cells in a dose-dependent manner, which was ascribed to AST-induced ROS in to inhibit the proliferative of cancer cells.

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