Ethanol‐Assisted Aqueous Enzymatic Extraction of Peony Seed Oil

2019 ◽  
Vol 96 (5) ◽  
pp. 595-606 ◽  
Author(s):  
Yuanyuan Song ◽  
Wenbin Zhang ◽  
Jing Wu ◽  
Habtamu Admassu ◽  
Junjun Liu ◽  
...  
LWT ◽  
2020 ◽  
Vol 133 ◽  
pp. 109992
Author(s):  
Hoang Chinh Nguyen ◽  
Dat Phu Vuong ◽  
Ngoc Thanh Tam Nguyen ◽  
Nguyen Phuong Nguyen ◽  
Chia-Hung Su ◽  
...  

Author(s):  
Nadirah Zawani MOHD NESFU ◽  
Hasnah OSMAN ◽  
Suriyati MOHAMAD ◽  
Lionel MUNIGLIA ◽  
Nicolas BROSSE ◽  
...  

The development of a sustainable product using natural resources will gain much attention nowadays. This work aimed to study the effect of aqueous enzymatic extraction (AEE) using a different ratio of enzyme cocktails HEL1 and X7 towards the production of Momordica charantia L. (M. charantia) seed oil and to identify the composition of the seeds’ lignocellulosic biomass (extracted-free materials, EFM). The M. charantia seed oil contained omega-3 fatty acids, such as stearidonic acid, that possess hypotriglyceridemic properties. The oil obtained was derivatized to fatty acid methyl esters (FAMEs) before biochemical quantification using gas chromatography (GC). The EFM was subjected to Soxhlet extraction and further analyzed to identify lignin, acid-soluble sugars, and hemicellulose composition. The results showed that the highest seed oil percentage, (6.26±0.53 %) was extracted from M2 sample using a combination of 5 % HEL1 to 1.25 % X7 enzyme cocktails, as compared to the extraction without enzyme (3.32±0.38 %). The percentage of stearidonic acid in the seed oil increased from 13.55 % (without enzyme) to 19.43 % (M2), proving that there were some change in terms of fatty acid composition in the seed oil.


Catalysts ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 748
Author(s):  
Veymar G. Tacias-Pascacio ◽  
Arnulfo Rosales-Quintero ◽  
Rafael C. Rodrigues ◽  
Daniel Castañeda-Valbuena ◽  
Pablo F. Díaz-Suarez ◽  
...  

In this study, aqueous enzymatic extraction (AEE) was evaluated during the process of obtaining oil from mamey sapote seed (OMSS). Viscozyme L enzyme complex was used at pH 4 and 50 °C during the optimization of the extraction process by central composite design and response surface methodology. Optimal conditions were: 3.5% (w/w) of enzyme (regarding the seed weight), 5.5 h of incubation time, 235 rpm of agitation rate, and 1:3.5 of solid-to-liquid ratio. These conditions enabled us to obtain an OMSS yield of 66%. No statistically significant differences were found in the fatty acid profile and physicochemical properties, such as the acid and iodine values and the percentage of free fatty acids, between the oil obtained by AEE or by the conventional solvent extraction (SE). However, the oxidative stability of the oil obtained by AEE (11 h) was higher than that obtained by SE (9.33 h), therefore, AEE, in addition to being an environmentally friendly method, produces a superior quality oil in terms of oxidative stability. Finally, the high oil content in mamey sapote seed, and the high percentage of oleic acid (around 50% of the total fatty acid) found in this oil, make it a useful edible vegetable oil.


2016 ◽  
Vol 61 (9) ◽  
pp. 162-168
Author(s):  
Hoang Than Hoai Thu ◽  
Dao Van Hoang ◽  
Doan Lan Phuong ◽  
Hoang Thi Bich ◽  
Pham Quoc Long

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1582
Author(s):  
Adchara Prommaban ◽  
Ratthida Kuanchoom ◽  
Natthidaporn Seepuan ◽  
Wantida Chaiyana

Pumpkin seed oil is a by-product, abundant in nutrients and bioactive components that promote several health benefits. This study aimed to compare chemical compositions, antioxidant, and pharmacological activities of pumpkin seed oils extracted from Cucurbita moschata Duch. Ex Poir. (PSO1) and Cucurbita moschata (Japanese pumpkin) (PSO2) by aqueous enzymatic extraction. An enzyme mixture consisting of pectinase, cellulase, and protease (1:1:1) was used in the enzymatic extraction process. Fatty acid composition of the oils was determined using fatty acid methyl ester/gas chromatographic-mass spectrometry. Antioxidant activity assays were measured by using stable free radical diphenylpicrylhydrazyl, radical cation 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonate, ferric reducing/antioxidant power, and ferric thiocyanate assay. Inhibition of enzymes involving skin aging and whitening process was investigated. Linoleic acid was a major component of all pumpkin seed oils. Additionally, there was also a significant amount of oleic acid, palmitic acid, and stearic acid detected. PSO2 possessed the highest antioxidant activities compared to PSO1 and commercial pumpkin seed oils (COM1 and COM2). Both PSO1 and PSO2 exhibited higher inhibitory effects on hyaluronidase, collagenase, and tyrosinase than the commercials. Therefore, aqueous enzymatic extraction could yield pumpkin seed oils with higher antioxidant, anti-aging, and whitening activities. This is beneficial for further pharmacological studies and can be used as a functional food for skin benefits.


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