The effect of phenyltin chlorides on osmotically induced erythrocyte haemolysis

2005 ◽  
Vol 19 (6) ◽  
pp. 736-741 ◽  
Author(s):  
Adam Miszta ◽  
Janina Gabrielska ◽  
Stanisław Przestalski ◽  
Marek Langner
Keyword(s):  
Erythrocyte Haemolysis

scholarly journals Haemolysis induced by tyrosine crystals: Modifiers and inhibitors

1976 ◽  
Vol 158 (1) ◽  
pp. 17-22 ◽  
Author(s):  
L A Goldsmith
Keyword(s):  
Polyethylene Glycol ◽  
Human Erythrocyte ◽  
Ionic Detergents ◽  
Erythrocyte Haemolysis

Tyrosine as a solid, but not in solution, caused human erythrocyte haemolysis. Haemolysis was increased with higher tyrosine concentrations and extended incubation times; it was greater at 37degrees than 4degreesC, and decreased by higher erythrocyte concentrations. Titration of phenolic groups on the surface of di-iodotyrosine crystals altered the extent of di-iodotyrosine-induced haemolysis. Haemolysis induced by tyrosine was inhibited by polyethylene glycol (mol.wt. 6000 or 20000) in a competitive fashion; polyoxyethylene/polyoxypropylene non-ionic detergents, polyvinylpyrrolidone (mol.wt. 40000 or 360000), 0.25-1.0M-NaC1, 0.25-1.0 M-KC1 and 0.25 M-NaSCN also inhibited haemolysis. H+-ion donation from the phenolic groups of tyrosine is suggested as part of the mechanism of haemolysis. Non-ionic detergents may inhibit tyrosine-crystal-induced haemolysis by binding the phenolic groups at the surface of the crystal.

Modulatory effect of methanolic extract ofVernonia amygdalina(MEVA) on tert-butyl hydroperoxide-induced erythrocyte haemolysis

2012 ◽  
pp. n/a-n/a ◽  
Author(s):  
Omolola A. Adesanoye ◽  
Olorunfemi R. Molehin ◽  
Adetutu A. Delima ◽  
Adeniyi S. Adefegha ◽  
Ebenezer O. Farombi
Keyword(s):  
Methanolic Extract ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Tert Butyl Hydroperoxide ◽  
Erythrocyte Haemolysis

scholarly journals The reaction of menadione with haemoglobin. Mechanism and effect of superoxide dismutase

1979 ◽  
Vol 179 (3) ◽  
pp. 665-673 ◽  
Author(s):  
C C Winterbourn ◽  
J K French ◽  
R F Claridge
Keyword(s):  
Superoxide Dismutase ◽  
Reaction Rate ◽  
Reduced Glutathione ◽  
Reverse Reaction ◽  
Main Reaction ◽  
Semiquinone Radical ◽  
Limited Extent ◽  
Thiol Groups ◽  
Reversible Formation ◽  
Erythrocyte Haemolysis

1. Menadione was found to react with both the haem groups and the beta-93 thiol groups of haemoglobin. 2. It oxidized the haem groups of oxyhaemoglobin, giving mainly methaemoglobin and a smaller amount of haemichrome. The reaction rate was decrease in the presence of catalase and markedly accelerated in the presence of superoxide dismutase. It is proposed that the overall reaction involves the initial reversible formation of methaemoglobin and the semiquinone, and that the effect of superoxide dismutase is to prevent the reverse reaction, by removing superoxide and hene O2-. E.s.r. evidence for the information of the semi-quinone and its reactions is presented. 3. The reaction of menadione with the beta-93 thiol groups of haemoglobin appeared to be similar to that with other thiols, forming the 3-thioether derivative of menadione, but it was also accompanied by reduction of methaemoglobin. This reduction was prevented by superoxide dismutase, but appeared to be caused by the semiquinone radical, which was produced as an intermediate. 4. Reduced glutathione functioned only to a limited extent as a scavenger of the menadione semiquinone. Its main reaction was directly with menadione to form the thioether. Ascorbate was a more efficient scavenger, and accelerated the oxidation of oxyhaemoglobin by menadione. 5. The significance of these findings in relation to menadione-induced erythrocyte haemolysis is discussed.

scholarly journals Comediation of Erythrocyte Haemolysis by Erythrocyte-Derived Microparticles and Complement during Malaria Infection

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Ransford Kyeremeh ◽  
Samuel Antwi-Baffour ◽  
Max Annani-Akollor ◽  
Jonathan Kofi Adjei ◽  
Otchere Addai-Mensah ◽  
...  
Keyword(s):  
Malaria Infection ◽  
Surface Protein ◽  
Surface Proteins ◽  
Sub Saharan Africa ◽  
In Vitro Assays ◽  
Intracellular Location ◽  
Fixed Volume ◽  
Global Threat ◽  
Erythrocyte Haemolysis

Background. Due to the sustained morbidity and mortality that malaria-associated anaemia imposes on patients, malaria is still a global threat, most especially, to residents in sub-Saharan Africa. Merozoite invasion and destruction of erythrocytes, a target for this study, have been necessary due to its unique nature and also since the erythrocytes suffer the most brunt of malarial infection leading to anaemia. The issue of malaria anaemia has to do with why uninfected RBCs get destroyed and even more so than infected ones. Studies have proposed that cytophilic anti-RSP2 (ring surface protein 2—merozoite rhoptry protein 2) antibodies present in sera enhance phagocytosis of RSP2-tagged RBCs by macrophages either directly or via complement, while others have proposed transfer of RSP2 to both infected and uninfected RBCs which may render them susceptible to phagocytosis. What is missing is the agent involved in the transfer of these parasite-induced surface proteins onto the uninfected RBCs, i.e., the mediator molecules. Considering the intracellular location of the parasite in the parasitophorous vacuolar membrane and the absence of a transport mechanism such as the Golgi apparatus within the mature RBC, since the latter has no nucleus, we propose that erythrocyte-derived microparticles (EMPs) may be the possible mediators. Aim. This study aimed at examining the immunological interactions between EMPs released during malarial infections and host erythrocytes that may lead to their lysis possibly through complement mediation. Methods. This was an experimental study during which malarial EMPs were isolated by differential centrifugation of malaria-positive plasma. This was followed by cell-based in vitro assays where malaria-positive EMPs were added to uninfected blood group “O” negative erythrocytes in the presence of complement and haemolysis checked for. Results and Conclusion. At a fixed volume of 50 μL complement, there were statistically significant (p<0.01) increases in mean percentage haemolysis as the volume of EMPs increased. Similarly, at a fixed volume of 50 μL EMPs, there were statistically significant (p<0.01) increases in mean percentage haemolysis with increasing volumes of complement. This was an indication that both complement and EMPs contribute significantly to uninfected erythrocyte haemolysis during malaria infection.

Vitamin C or E Supplementation Ameliorates Nigerian Bonny Light Crude Oil-Induced Erythrocyte Haemolysis in Male Wistar Rats

2013 ◽  
Vol 1 (3) ◽  
pp. 44 ◽  
Author(s):  
Sunday Otu Ita ◽  
Esther Oluwasola Aluko ◽  
Dara Ezekiel Atang ◽  
Atim Atim Bassey Antai ◽  
Eme E. Osim
Keyword(s):  
Vitamin C ◽  
Crude Oil ◽  
Wistar Rats ◽  
Light Crude Oil ◽  
Male Wistar Rats ◽  
Erythrocyte Haemolysis
Sign in / Sign up

Export Citation Format

Share Document