Binding kinetics of magnetic nanoparticles on latex beads studied by magnetorelaxometry

2004 ◽  
Vol 18 (10) ◽  
pp. 542-547 ◽  
Author(s):  
D. Eberbeck ◽  
Ch. Bergemann ◽  
S. Hartwig ◽  
U. Steinhoff ◽  
L. Trahms
Keyword(s):  
Magnetic Nanoparticles ◽  
Binding Kinetics ◽  
Latex Beads ◽  
Kinetics Of

Binding kinetics of magnetic nanoparticles on latex beads and yeast cells studied by magnetorelaxometry

2005 ◽  
Vol 289 ◽  
pp. 435-438 ◽  
Author(s):  
Dietmar Eberbeck ◽  
Christian Bergemann ◽  
Stefan Hartwig ◽  
Uwe Steinhoff ◽  
Lutz Trahms
Keyword(s):  
Magnetic Nanoparticles ◽  
Binding Kinetics ◽  
Yeast Cells ◽  
Latex Beads ◽  
Kinetics Of

scholarly journals Characterization of a Humanized Monoclonal Antibody Recognizing Clumping Factor A Expressed by Staphylococcus aureus

2005 ◽  
Vol 73 (8) ◽  
pp. 5229-5232 ◽  
Author(s):  
Paul J. Domanski ◽  
Pratiksha R. Patel ◽  
Arnold S. Bayer ◽  
Li Zhang ◽  
Andrea E. Hall ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Monoclonal Antibody ◽  
Infective Endocarditis ◽  
Binding Kinetics ◽  
Vancomycin Therapy ◽  
Latex Beads ◽  
Humanized Monoclonal Antibody ◽  
Kinetics Of ◽  
Therapeutic Model

ABSTRACT We report the humanization and characterization of monoclonal antibody (MAb) T1-2 or tefibazumab, a monoclonal antibody that recognizes clumping factor A expressed on the surface of Staphylococcus aureus. We demonstrate that the binding kinetics of MAb T1-2 is indistinguishable compared to that of its murine parent. Furthermore, MAb T1-2 is shown to enhance the opsonophagocytic uptake of ClfA-coated latex beads, protect against an intravenous challenge in a prophylactic model of rabbit infective endocarditis, and enhance the efficacy of vancomycin therapy in a therapeutic model of established infective endocarditis.

A new real-time method for investigation of affinity properties and binding kinetics of magnetic nanoparticles

2015 ◽  
Vol 380 ◽  
pp. 231-235 ◽  
Author(s):  
Alexey V. Orlov ◽  
Maxim P. Nikitin ◽  
Vera A. Bragina ◽  
Sergey L. Znoyko ◽  
Marina N. Zaikina ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Real Time ◽  
Binding Kinetics ◽  
Kinetics Of

Video-microscopic measurement of cell-substratum traction forces generated by locomoting keratocytes

1993 ◽  
Vol 51 ◽  
pp. 188-189
Author(s):  
Tim Oliver ◽  
Michelle Leonard ◽  
Juliet Lee ◽  
Akira Ishihara ◽  
Ken Jacobson
Keyword(s):  
Silicone Rubber ◽  
Molecular Motors ◽  
Video Frame ◽  
Traction Forces ◽  
Cell Traction Forces ◽  
Latex Beads ◽  
Cell Traction ◽  
Local Cell ◽  
Microscopic Measurement ◽  
Kinetics Of

We are using video-enhanced light microscopy to investigate the pattern and magnitude of forces that fish keratocytes exert on flexible silicone rubber substrata. Our goal is a clearer understanding of the way molecular motors acting through the cytoskeleton co-ordinate their efforts into locomotion at cell velocities up to 1 μm/sec. Cell traction forces were previously observed as wrinkles(Fig.l) in strong silicone rubber films by Harris.(l) These forces are now measureable by two independant means.In the first of these assays, weakly crosslinked films are made, into which latex beads have been embedded.(Fig.2) These films report local cell-mediated traction forces as bead displacements in the plane of the film(Fig.3), which recover when the applied force is released. Calibrated flexible glass microneedles are then used to reproduce the translation of individual beads. We estimate the force required to distort these films to be 0.5 mdyne/μm of bead movement. Video-frame analysis of bead trajectories is providing data on the relative localisation, dissipation and kinetics of traction forces.

Binding kinetics of fluorescent bisphosphonates as a tool for monitoring bone dynamics in vivo

2013 ◽  
Author(s):  
Robert Tower ◽  
Graeme Campbell ◽  
Marc Muller ◽  
Olga Will ◽  
Frederieka Grundmann ◽  
...  
Keyword(s):  
Binding Kinetics ◽  
Kinetics Of ◽  
Bone Dynamics

Influence of Brain Gangliosides on the Formation and Properties of Supported Lipid Bilayers for Binding Kinetics Studies

2018 ◽  
Author(s):  
Luke Jordan ◽  
Nathan Wittenberg
Keyword(s):  
Lipid Bilayers ◽  
Binding Kinetics ◽  
Supported Lipid Bilayers ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Supported Lipid Bilayer ◽  
Head Groups ◽  
Lipid Diffusion ◽  
Kinetics Of ◽  
Brain Gangliosides

This is a comprehensive study of the effects of the four major brain gangliosides (GM1, GD1b, GD1a, and GT1b) on the adsorption and rupture of phospholipid vesicles on SiO2 surfaces for the formation of supported lipid bilayer (SLB) membranes. Using quartz crystal microbalance with dissipation monitoring (QCM-D) we show that gangliosides GD1a and GT1b significantly slow the SLB formation process, whereas GM1 and GD1b have smaller effects. This is likely due to the net ganglioside charge as well as the positions of acidic sugar groups on ganglioside glycan head groups. Data is included that shows calcium can accelerate the formation of ganglioside-rich SLBs. Using fluorescence recovery after photobleaching (FRAP) we also show that the presence of gangliosides significantly reduces lipid diffusion coefficients in SLBs in a concentration-dependent manner. Finally, using QCM-D and GD1a-rich SLB membranes we measure the binding kinetics of an anti-GD1a antibody that has similarities to a monoclonal antibody that is a hallmark of a variant of Guillain-Barre syndrome.

scholarly journals CO Binding Kinetics of Human Cytochrome P450 3A4

1995 ◽  
Vol 270 (10) ◽  
pp. 5014-5018 ◽  
Author(s):  
Aditya P. Koley ◽  
Jeroen T. M. Buters ◽  
Richard C. Robinson ◽  
Allen Markowitz ◽  
Fred K. Friedman
Keyword(s):  
Cytochrome P450 ◽  
Binding Kinetics ◽  
Cytochrome P450 3A4 ◽  
Human Cytochrome ◽  
Kinetics Of

scholarly journals Dissecting the impact of target-binding kinetics of protein binders on tumor localization

iScience ◽  
2021 ◽  
Vol 24 (2) ◽  
pp. 102104
Author(s):  
Yunjin Song ◽  
Hoibin Jeong ◽  
Song-Rae Kim ◽  
Yiseul Ryu ◽  
Jonghwi Baek ◽  
...  
Keyword(s):  
Binding Kinetics ◽  
Tumor Localization ◽  
Protein Binders ◽  
Target Binding ◽  
The Impact ◽  
Kinetics Of
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