Chemical characterization and therapeutic properties of Achillea biebersteinii leaf aqueous extract synthesized copper nanoparticles against methamphetamine‐induced cell death in PC12: A study in the nanotechnology and neurology fields

2020 ◽  
Vol 34 (4) ◽  
Author(s):  
Gang Wang ◽  
Ahmad Ahmeda ◽  
Zahra Malek ◽  
Shirin Mansooridara ◽  
Akram Zangeneh ◽  
...  
Keyword(s):  
Cell Death ◽  
Aqueous Extract ◽  
Copper Nanoparticles ◽  
Chemical Characterization ◽  
Leaf Aqueous Extract ◽  
Achillea Biebersteinii ◽  
Therapeutic Properties

scholarly journals RETRACTED ARTICLE: Suppressor capacity of copper nanoparticles biosynthesized using Crocus sativus L. leaf aqueous extract on methadone-induced cell death in adrenal phaeochromocytoma (PC12) cell line

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Peng Zhang ◽  
Jian Cui ◽  
Shirin Mansooridara ◽  
Atoosa Shahriyari Kalantari ◽  
Akram Zangeneh ◽  
...  
Keyword(s):  
Cell Death ◽  
Nervous System ◽  
Membrane Potential ◽  
Inflammatory Cytokines ◽  
Aqueous Extract ◽  
Copper Nanoparticles ◽  
Mitochondrial Membrane ◽  
Caspase 3 ◽  
Crocus Sativus ◽  
Leaf Aqueous Extract

AbstractIn this research, we prepared and formulated a neuroprotective supplement (copper nanoparticles in aqueous medium utilizing Crocus sativus L. Leaf aqueous extract) for determining its potential against methadone-induced cell death in PC12. The results of chemical characterization tests i.e., FE-SEM, FT-IR, XRD, EDX, TEM, and UV–Vis spectroscopy revealed that the study showed that copper nanoparticles were synthesized in the perfect way possible. In the TEM and FE-SEM images, the copper nanoparticles were in the mean size of 27.5 nm with the spherical shape. In the biological part of the present research, the Rat inflammatory cytokine assay kit was used to measure the concentrations of inflammatory cytokines. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) test was used to show DNA fragmentation and apoptosis. Caspase-3 activity was assessed by the caspase activity colorimetric assay kit and mitochondrial membrane potential was studied by Rhodamine123 fluorescence dye. Also, the cell viability of PC12 was measured by trypan blue assay. Copper nanoparticles-treated cell cutlers significantly (p ≤ 0.01) decreased the inflammatory cytokines concentrations, caspase-3 activity, and DNA fragmentation and they raised the cell viability and mitochondrial membrane potential in the high concentration of methadone-treated PC12 cells. The best result of neuroprotective properties was seen in the high dose of copper nanoparticles i.e., 4 µg. According to the above results, copper nanoparticles containing C. sativus leaf aqueous extract can be used in peripheral nervous system treatment as a neuroprotective promoter and central nervous system after approving in the clinical trial studies in humans.

scholarly journals Retraction Note: Suppressor capacity of copper nanoparticles biosynthesized using Crocus sativus L. leaf aqueous extract on methadone-induced cell death in adrenal phaeochromocytoma (PC12) cell line

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Peng Zhang ◽  
Jian Cui ◽  
Shirin Mansooridara ◽  
Atoosa Shahriyari Kalantari ◽  
Akram Zangeneh ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Line ◽  
Aqueous Extract ◽  
Copper Nanoparticles ◽  
Pc12 Cell ◽  
Crocus Sativus ◽  
Pc12 Cell Line ◽  
Leaf Aqueous Extract ◽  
Retraction Notice ◽  
Crocus Sativus L

This article has been retracted. Please see the Retraction Notice for more detail: https://doi.org/10.1038/s41598-020-77741-4.

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