scholarly journals Isolation of Circulating Biomarkers for Liquid Biopsy using Immunoaffinity‐Based Stimuli‐Responsive Hybrid Hydrogel Beads

2021 ◽  
Author(s):  
Yoon-Tae Kang ◽  
Young Jun Kim ◽  
Brittany Rupp ◽  
Emma Purcell ◽  
Thomas Hadlock ◽  
...  
Keyword(s):  
Liquid Biopsy ◽  
Stimuli Responsive ◽  
Circulating Biomarkers ◽  
Hybrid Hydrogel ◽  
Hydrogel Beads

scholarly journals Circulating Biomarkers of Response and Toxicity of Immunotherapy in Advanced Non-Small Cell Lung Cancer (NSCLC): A Comprehensive Review

Cancers ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1794
Author(s):  
Alice Indini ◽  
Erika Rijavec ◽  
Francesco Grossi
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Liquid Biopsy ◽  
Cytotoxic T Lymphocyte ◽  
Checkpoint Inhibitors ◽  
Small Cell ◽  
Circulating Biomarkers ◽  
Small Cell Lung ◽  
Nsclc Patients

Immune checkpoint inhibitors (ICIs) targeting the programmed cell death (PD)-1 protein and its ligand, PD-L1, and cytotoxic T-lymphocyte-associated antigen (CTLA)-4, have revolutionized the management of patients with advanced non-small cell lung cancer (NSCLC). Unfortunately, only a small portion of NSCLC patients respond to these agents. Furthermore, although immunotherapy is usually well tolerated, some patients experience severe immune-related adverse events (irAEs). Liquid biopsy is a non-invasive diagnostic procedure involving the isolation of circulating biomarkers, such as circulating tumor cells (CTC), cell-free DNA (cfDNA), and microRNAs (miRNAs). Thanks to recent advances in technologies, such as next-generation sequencing (NGS) and digital polymerase chain reaction (dPCR), liquid biopsy has become a useful tool to provide baseline information on the tumor, and to monitor response to treatments. This review highlights the potential role of liquid biomarkers in the selection of NSCLC patients who could respond to immunotherapy, and in the identification of patients who are most likely to experience irAEs, in order to guide improvements in care.

scholarly journals Nanoporous Sodium Carboxymethyl Cellulose-g-poly (Sodium Acrylate)/FeCl3 Hydrogel Beads: Synthesis and Characterization

Gels ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. 49
Author(s):  
Bijender Kumar ◽  
Ruchir Priyadarshi ◽  
Sauraj ◽  
Farha Deeba ◽  
Anurag Kulshreshtha ◽  
...  
Keyword(s):  
Carboxymethyl Cellulose ◽  
Sodium Carboxymethyl Cellulose ◽  
Sodium Acrylate ◽  
X Ray Diffraction ◽  
Hybrid Hydrogel ◽  
X Ray ◽  
Hydrogel Beads ◽  
Force Microscopy ◽  
Atomic Force ◽  
Potential Applications

Novel sodium carboxymethyl cellulose-g-poly (sodium acrylate)/Ferric chloride (CMC-g-PNaA/FeCl3) nanoporous hydrogel beads were prepared based on the ionic cross-linking between CMC-g-PNaA and FeCl3. The structure of CMC and CMC-g-PNaA were elucidated by Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR) spectroscopy, and the elemental composition was analyzed by energy dispersive X-ray analysis (EDX). The physicochemical properties of the CMC-g-PNaA/FeCl3 hydrogel beads were analyzed by X-ray diffraction (XRD), scanning electron microscopy (SEM), atomic force microscopy (AFM) and thermogravimetric analysis (TGA). The swelling percentage of hydrogel beads was studied at different time periods. The obtained CMC-g-PNaA/FeCl3 hydrogel beads exhibited a higher nanoporous morphology than those of CMC-g-PNaA and CMC beads. Furthermore, an AFM image of the CMC-g-PNaA/FeCl3 beads shows granule type topology. Compared to the CMC-g-PNaA (189 °C), CMC-g-PNaA/FeCl3 hydrogel beads exhibited improvement in thermal stability (199 °C). Furthermore, CMC-g-PNaA/FeCl3 hydrogel beads depicted a higher swelling percentage capacity of around 1452%, as compared to CMC-g-PNaA (1096%). Moreover, this strategy with preliminary results could be useful for the development of polysaccharide-based hybrid hydrogel beads for various potential applications.

Cancer Diagnosis: From Tumor to Liquid Biopsy and Beyond

Lab on a Chip ◽  
2018 ◽  
Author(s):  
Ramanathan Vaidyanathan ◽  
Ren Hao Soon ◽  
Pan Zhang ◽  
Kuan Jiang ◽  
Chwee Teck Lim
Keyword(s):  
Real Time ◽  
Cancer Diagnosis ◽  
Liquid Biopsy ◽  
Patient Monitoring ◽  
Circulating Biomarkers ◽  
Less Invasive

Technological advancements in research on circulating biomarkers from patient derived blood have enabled a less invasive means of diagnosing non-hematologic cancers. Considered a more practical way of real-time patient monitoring...

Enhanced mechanical stability and sensitive swelling performance of chitosan/yeast hybrid hydrogel beads

2016 ◽  
Vol 40 (4) ◽  
pp. 3350-3362 ◽  
Author(s):  
Diejing Feng ◽  
Bo Bai ◽  
Honglun Wang ◽  
Yourui Suo
Keyword(s):  
Mechanical Stability ◽  
Hybrid Hydrogel ◽  
Hydrogel Beads ◽  
Chitosan Hydrogels ◽  
Yeast Hybrid

Up to the present time, improving the mechanical stability of hydrogel beads is still a challenging task for future applications of chitosan hydrogels.

scholarly journals Improving Printability of a Thermoresponsive Hydrogel Biomaterial Ink by Nanoclay Addition

2020 ◽  
Author(s):  
Chen Hu ◽  
Lukas Hahn ◽  
Mengshi Yang ◽  
Alexander Altmann ◽  
Philipp Stahlhut ◽  
...  
Keyword(s):  
Block Copolymer ◽  
Viscoelastic Properties ◽  
Critical Properties ◽  
Stimuli Responsive ◽  
Hybrid Hydrogel ◽  
Thermoresponsive Hydrogel ◽  
Enhanced Resolution ◽  
Copolymer Poly ◽  
Significant Attention ◽  
Made In

As a promising biofabrication technology, extrusion-based bioprinting has gained significant attention in the last decade and major advances have been made in the development of bioinks. However suitable synthetic and stimuli-responsive bioinks are underrepresented in this context. <a>In this work, we described a hybrid system of nanoclay Laponite XLG and thermoresponsive block copolymer poly(2-methyl-2-oxazoline)-<i>b</i>-poly(2-<i>n</i>-propyl-2-oxazine) (PMeOx-<i>b</i>-PnPrOzi) as a novel biomaterial ink, and discussed its critical properties relevant for extrusion-based bioprinting, including viscoelastic properties and printability.</a> <a>The hybrid hydrogel retains the thermogelling properties but is strengthened by the added clay (over 5 kPa of storage modulus, and 240 Pa of yield stress). Importantly, the shear-thinning character is further enhanced, which, in combination with very rapid viscosity recovery (~1 s) and structure recovery (~10 s) is highly beneficial for extrusion-based 3D printing. Accordingly, various 3D patterns could be printed with markedly enhanced resolution and shape fidelity compared to the biomaterial ink without added clay.</a>

scholarly journals Survival and Behavior of Encapsulated Probiotics (Lactobacillus plantarum) in Calcium-Alginate-Soy Protein Isolate-Based Hydrogel Beads in Different Processing Conditions (pH and Temperature) and in Pasteurized Mango Juice

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Ong-Ard Praepanitchai ◽  
Athapol Noomhorm ◽  
Anil Kumar Anal
Keyword(s):  
Lactobacillus Plantarum ◽  
Soy Protein ◽  
Soy Protein Isolate ◽  
Protein Isolate ◽  
Heat Processing ◽  
Hybrid Hydrogel ◽  
Hydrogel Beads ◽  
Acidic Conditions ◽  
Mango Juice ◽  
And Behavior

Hybrid alginate-soy protein isolate-based hydrogel beads were prepared and evaluated to enhance the survival of the encapsulated probiotics (Lactobacillus plantarum) during heat processing to incorporate in mango juice. The solutions of sodium alginate-soy protein isolate (SA-SPI) with probiotic cells were dropped into the gelation bath containing calcium chloride (3% w/v) solution to develop various types of hydrogel beads. The level of survival of probiotics in encapsulated beads under acidic conditions (pH 2, 3, and 6.5) and bile salt (0.5 and 1.0% w/v) was evaluated. The survival of the encapsulated probiotics to thermal processing was evaluated by treating the beads in saline solution (0.9% w/v) at 30, 50, 63, and 72°C. The encapsulated probiotic bacteria were found alive even after treatment at 72°C for 90 s. Most of the free cells did not survive at the temperature higher than 50°C and very low pH (pH 2 and 3). The survival of probiotic cells was found higher with the hybrid hydrogel beads containing alginate and soy protein isolate (1:8 w/w). Furthermore, mango juice fortified with encapsulated L. plantarum in hydrogel beads was subjected to thermal pasteurization at 72°C for 90 s.

Determination of AR-V7 in plasma in patients with metastatic prostate cancer using capillary Nano-immunoassay.

2019 ◽  
Vol 37 (15_suppl) ◽  
pp. e16574-e16574
Author(s):  
Alvaro Virseda ◽  
Juan Luis Garcia ◽  
Sara Marcos Asensio ◽  
Javier Garcia Garcia ◽  
Manuel Herrero POLO ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Liquid Biopsy ◽  
Clinical Characteristics ◽  
Analytical Data ◽  
Biological Data ◽  
Circulating Biomarkers ◽  
Patient Table ◽  
Lactate Levels ◽  
Androgen Receptor Splice Variant

e16574 Background: Liquid biopsy has the potential to provide information about cancers without invasive biopsy, using circulating biomarkers. The aim of present study was determinate the presence of androgen receptor splice variant 7 (AR-V7) in plasma using capillary nano-immunoassay in metastasis PCa and correlation with clinical and analytical data. Methods: Whole blood (10 ml) was collected and processed in EDTA tubes. Previously to determinate the presence of ARV-7 in plasma Capillary Electrophoresis Immunoassay or SimpleWestern analyses were performed using the WES™ machine. With this technique, proteins are separated by size, immobilised and probed with specific antibodies. The primary antibody used was Anti-Androgen Receptor antibody. Results: The study included 31 patients with diagnostic of PCa metatasic . Clinical characteristics of the patients were described in the table. AR-V7 signal was detected in 11 cases (36%). Twenty samples (64%) were considered as negative. The most of patients with expression positive for AR-V7, showed a Gleason score ≥ 8 (64% of cases). In AR-V7+, hemoglobine and dehydrogenase lactate levels were statistically significant (p < 0,05) respect to ARV7-. So, in AR-V7+ patients showed increased of FA and Albumin. Conclusions: Assessing the presence of AR-V7 in plasma from PC patients is feasible by a novel capillary nano-immunoassay. AR-V7 was observed in 36% of the PCA metastasic. These findings lay the foundations for liquid biopsy as a means of obtaining biological data in a more convenient way for the patient.[Table: see text]

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