The Utilization of Lanthipeptide Synthetases Is a General Strategy for the Biosynthesis of 2‐Aminovinyl‐Cysteine Motifs in Thioamitides**

2020 ◽  
Author(s):  
Jingxia Lu ◽  
Yuan Wu ◽  
Yuqing Li ◽  
Huan Wang
Keyword(s):  
General Strategy ◽  
Cysteine Motifs

scholarly journals Lanthipeptide Synthetases Participate the Biosynthesis of 2-Aminovinyl-Cysteine Motifs in Thioamitides

2020 ◽  
Author(s):  
Jingxia Lu ◽  
Yuan Wu ◽  
Jiao Li ◽  
Yuqing Li ◽  
Yingying Zhang ◽  
...  
Keyword(s):  
Natural Products ◽  
Gene Clusters ◽  
General Strategy ◽  
Biosynthetic Gene ◽  
Class Iii ◽  
Biosynthetic Gene Clusters ◽  
Bioactive Natural Products ◽  
Modified Peptides ◽  
Cysteine Motifs ◽  
The Way

ABSTRACTThioamitides are a group of ribosomally synthesized and post-translational modified peptides with potent antiproliferative and pro-apoptotic activities. Their biosynthesis remains largely unknown, especially for the characteristic C-terminal 2-aminovinyl-Cysteine (AviCys) motifs. Herein, we report the discovery that homologs of class III lanthipeptide synthetases (LanKCts)encoded outside putative thioamitide biosynthetic gene clusters (BGCs) fully dehydrate the precursor peptides. Remarkably, LanKCt enzymes bind tightly to cysteine decarboxylases encoded inside thioamitide BGCs, and the resulting complex complete the macrocyclization of AviCys rings. Furthermore, LanKCt enzymes are present in the genomes of many thioamitide-producing strains and are functional when in complex with cysteine decarboxylases to produce AviCys macrocycles. Thus, our study reveals the participation of lanthipeptide synthetases as a general strategy for dehydration and AviCys formation during thioamitides biosynthesis and thus paves the way for the bioengineering of this class of bioactive natural products.

Towards Systematization

1977 ◽  
Vol 16 (03) ◽  
pp. 125-130 ◽  
Author(s):  
P. L. Reichertz
Keyword(s):  
Clinical Medicine ◽  
Medical Science ◽  
User Involvement ◽  
Empirical Science ◽  
Target System ◽  
General Strategy ◽  
System Construction ◽  
Formal Approach ◽  
Methodology Development ◽  
Planning Development

Data processing has become an important tool in theoretical and clinical medicine. The main categories of applications are : information analysis, (bio)signal processing and the field of information logistics (information systems).The problems encountered lie in the discrepancy of the basic methods of a formal approach to an empirical science, the complexity of the target system and the system ecology, i.e. the involvement of the user and the system environment during system construction and utilization.Possible solutions to these problems are the application of system techniques, inductive planning, development of medical methodology, development of methods and techniques for user involvement and assessment of motivation and education and educational planning.The necessary general strategy in the development in medical informatics is seen in the continuing systematization of the theoretical and practical approach. It is estimated that this will eventually contribute to the systematization of medical science and practice.

Tetraphenylbenzene-based AIEgens: the Horizontally Oriented Emitters for highly Efficient Non-doped Deep Blue OLEDs and Host for High-Performance Hybrid WOLEDs

2020 ◽  
Author(s):  
Pengbo Han ◽  
Zeng Xu ◽  
Chengwei Lin ◽  
Dongge Ma ◽  
Anjun Qin ◽  
...  
Keyword(s):  
High Performance ◽  
General Strategy ◽  
Flat Panel Displays ◽  
Light Emitting ◽  
Horizontal Orientation ◽  
Deep Blue ◽  
Full Color ◽  
High Emission ◽  
White Oleds ◽  
Low Efficiency

Deep blue organic-emitting fluorophores are crucial for application in white lighting and full color flat-panel displays but emitters with high color quality and efficiency are rare. Herein, novel deep blue AIE luminogens (AIEgens) with various donor units and an acceptor of cyano substituted tetraphenylbenzene (TPB) cores were developed and used to fabricate non-doped deep blue and hybrid white organic light-emitting diodes (OLEDs). Benefiting from its high emission efficiency and high proportion of horizontally oriented dipoles in the film state, the non-doped deep blue device based on CN-TPB-TPA realized a maximum external quantum efficiency 7.27%, with a low efficiency roll-off and CIE coordinates of (0.15, 0.08). Moreover, efficient two-color hybrid warm white OLEDs (CIE<sub>x,y</sub> = 0.43, 0.45) were achieved using CN-TPB-TPA as the blue-emitting layer and phosphor doped host, which realized maximum current, power, external quantum efficiencies 58.0 cd A<sup>-1</sup>, 60.7 lm W<sup>-1</sup> and 19.1%, respectively. This work provides a general strategy to achieve high performance, stable deep blue and hybrid white OLEDs by construction of AIEgens with excellent horizontal orientation

A One-Pot Chemoenzymatic Synthesis of (2S,4R)-4-Methylproline Enables the First Total Synthesis of Antiviral Lipopeptide Cavinafungin B

2018 ◽  
Author(s):  
Christian R. Zwick ◽  
Hans Renata
Keyword(s):  
Total Synthesis ◽  
Natural Product ◽  
Complex Molecule ◽  
Molecular Target ◽  
Chemoenzymatic Synthesis ◽  
General Strategy ◽  
One Pot

We report an efficient ten-step synthesis of antiviral natural product cavinafungin B in 37% overall yield. By leveraging a one-pot chemoenzymatic synthesis of (2S,4R)-4-methylproline and oxazolidine-tethered (Rink-Boc-ATG-resin) SPPS methodology, the assembly of our molecular target could be conducted in an efficient manner.This general strategy could prove amenable to the construction of other natural and unnatural linear lipopeptides. The value of incorporating biocatalytic steps in complex molecule synthesis is highlighted by this work.

Visible-Light Photosensitized Aryl and Alkyl Decarboxylative Carbon-Heteroatom and Carbon-Carbon Bond Formations

2019 ◽  
Author(s):  
Tuhin Patra ◽  
Satobhisha Mukherjee ◽  
Jiajia Ma ◽  
Felix Strieth-Kalthoff ◽  
Frank Glorius
Keyword(s):  
Energy Transfer ◽  
Visible Light ◽  
Alkyl Radical ◽  
General Strategy ◽  
Alkyl Radicals ◽  
Bond Forming ◽  
Radical Trapping ◽  
Carbon Carbon Bond ◽  
Radical Generation ◽  
Trapping Agent

<sub>A general strategy to access both aryl and alkyl radicals by photosensitized decarboxylation of the corresponding carboxylic acids esters has been developed. An energy transfer mediated homolysis of unsymmetrical sigma-bonds for a concerted fragmentation/decarboxylation process is involved. As a result, an independent aryl/alkyl radical generation step enables a series of key C-X and C-C bond forming reactions by simply changing the radical trapping agent.</sub>

The Total Synthesis of Rhabdastrellic Acid A

2018 ◽  
Author(s):  
Yaroslav Boyko ◽  
Christopher Huck ◽  
David Sarlah
Keyword(s):  
Total Synthesis ◽  
Late Stage ◽  
Cross Coupling ◽  
Side Chains ◽  
General Strategy ◽  
Modular Approach ◽  
Linear Sequence ◽  
Generating Sequence ◽  
First Time

<div>The first total synthesis of rhabdastrellic acid A, a highly cytotoxic isomalabaricane triterpenoid, has been accomplished in a linear sequence of 14 steps from commercial geranylacetone. The prominently strained <i>trans-syn-trans</i>-perhydrobenz[<i>e</i>]indene core characteristic of the isomalabaricanes is efficiently accessed in a selective manner for the first time through a rapid, complexity-generating sequence incorporating a reductive radical polyene cyclization, an unprecedented oxidative Rautenstrauch cycloisomerization, and umpolung 𝛼-substitution of a <i>p</i>-toluenesulfonylhydrazone with in situ reductive transposition. A late-stage cross-coupling in concert with a modular approach to polyunsaturated side chains renders this a general strategy for the synthesis of numerous family members of these synthetically challenging and hitherto inaccessible marine triterpenoids.</div>

Public Libraries: Their Role in Smart City Strategies

2020 ◽  
Vol 58 (2(116)) ◽  
pp. 26-43
Author(s):  
Mariusz Luterek
Keyword(s):  
Smart City ◽  
Information Science ◽  
Public Libraries ◽  
Smart Cities ◽  
National Language ◽  
General Strategy ◽  
Language Version ◽  
Strategic Plans ◽  
Unique Approach ◽  
New Perspective

  Purpose/Thesis: This paper examines the position of public libraries in smart city strategies. To that end, I verify two hypotheses, H1: Cities analyzed employ strategic plans to define their path to “smartness”, and H2: Public libraries are a part of these strategies. Approach/Methods: Top 30 cities from the ranking of IESE Cities in Motion Index 2019 were se­lected. The hypotheses were tested through the analysis of strategy documents and web portals. In most cases, the analysis relied on English versions of said documents/portals, occasionally compared with the national language version . Results and conclusions: The process of verifying the first hypothesis led to identifying four groups: G1, comprising cities with a general strategy, presumed to include smart initiatives (3 cities), G2: cities with a separate “smart city” strategy, published on their own portal, or a related website (15 cities); G3: cities with subsites/portals briefly summarizing their activities in the area of ‘smart’ development (10 cities), and G4: cities with many sectoral strategies, presumed to include smart initiatives (2 ci­ties). The analysis allowed the identification of a number of areas in which public libraries already contribute to smart development: smart building, smart infrastructure, smart services, digital skills and life-long learning, sustainability, creativity, digital citizenship and smart business Originality/Value: Although many library and information science scholars study smart cities, no similar study has been conducted, and therefore, this paper, with its unique approach, offers a new perspective on the discussion on smart libraries.

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