An Extrinsic‐Pore‐Containing Molecular Sieve Film: A Robust, High‐Throughput Membrane Filter

2020 ◽  
Author(s):  
Sungwon Hong ◽  
Yanghwan Jeong ◽  
Hionsuck Baik ◽  
Nakwon Choi ◽  
Alex C. K. Yip ◽  
...  
Keyword(s):  
High Throughput ◽  
Molecular Sieve ◽  
Membrane Filter

An Extrinsic‐Pore‐Containing Molecular Sieve Film: A Robust, High‐Throughput Membrane Filter

2020 ◽  
Author(s):  
Sungwon Hong ◽  
Yanghwan Jeong ◽  
Hionsuck Baik ◽  
Nakwon Choi ◽  
Alex C. K. Yip ◽  
...  
Keyword(s):  
High Throughput ◽  
Molecular Sieve ◽  
Membrane Filter

High-Throughput Synthesis and Catalytic Properties of a Molecular Sieve with 18- and 10-Member Rings.

ChemInform ◽  
2007 ◽  
Vol 38 (3) ◽  
Author(s):  
Avelino Corma ◽  
Maria J. Diaz-Cabanas ◽  
Jose Luis Jorda ◽  
Cristina Martinez ◽  
Manuel Moliner
Keyword(s):  
High Throughput ◽  
Molecular Sieve ◽  
Catalytic Properties

High-throughput synthesis and catalytic properties of a molecular sieve with 18- and 10-member rings

Nature ◽  
2006 ◽  
Vol 443 (7113) ◽  
pp. 842-845 ◽  
Author(s):  
Avelino Corma ◽  
María J. Díaz-Cabañas ◽  
José Luis Jordá ◽  
Cristina Martínez ◽  
Manuel Moliner
Keyword(s):  
High Throughput ◽  
Molecular Sieve ◽  
Catalytic Properties

High Throughput Cell-Free Extraction of Plasma by an Integrated Microfluidic Device Combining Inertial Focusing and Membrane

2017 ◽  
Vol 139 (5) ◽  
Author(s):  
Jun Zhang ◽  
Sheng Yan ◽  
Dan Yuan ◽  
Gursel Alici ◽  
Nam-Trung Nguyen ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Microfluidic Device ◽  
High Throughput ◽  
Membrane Filtration ◽  
Membrane Filter ◽  
Whole Body ◽  
Inertial Focusing ◽  
High Efficient ◽  
Plasma Extraction

Plasma is a host of numerous analytes such as proteins, metabolites, circulating nucleic acids (CNAs), and pathogens, and it contains massive information about the functioning of the whole body, which is of great importance for the clinical diagnosis. Plasma needs to be completely cell-free for effective detection of these analytes. The key process of plasma extraction is to eliminate the contamination from blood cells. Centrifugation, a golden standard method for blood separation, is generally lab-intensive, time consuming, and even dangerous to some extent, and needs to be operated by well-trained staffs. Membrane filtration can filter cells very effectively according to its pore size, but it is prone to clogging by dense particle concentration and suffers from limited capacity of filtration. Frequent rinse is lab-intensive and undesirable. In this work, we proposed and fabricated an integrated microfluidic device that combined particle inertial focusing and membrane filter for high efficient blood plasma separation. The integrated microfluidic device was evaluated by the diluted (×1/10, ×1/20) whole blood, and the quality of the extracted blood plasma was measured and compared with that from the standard centrifugation. We found that the quality of the extracted blood plasma from the proposed device can be equivalent to that from the standard centrifugation. This study demonstrates a significant progress toward the practical application of inertial microfluidics with membrane filter for high-throughput and highly efficient blood plasma extraction.

High throughput development of one carbon molecular sieve for many gas separations

2015 ◽  
Vol 206 ◽  
pp. 207-216 ◽  
Author(s):  
Junqiang Liu ◽  
Chan Han ◽  
Mark McAdon ◽  
Janet Goss ◽  
Kyle Andrews
Keyword(s):  
High Throughput ◽  
Molecular Sieve ◽  
Gas Separations ◽  
Carbon Molecular Sieve

High Throughput Cell-Free Extraction of Plasma by an Integrated Microfluidic Device Combining Inertial Microfluidics and Membrane

2016 ◽  
Author(s):  
Jun Zhang ◽  
Sheng Yan ◽  
Dan Yuan ◽  
Gursel Alici ◽  
Nam-trung Nguyen ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Microfluidic Device ◽  
High Throughput ◽  
Membrane Filtration ◽  
Membrane Filter ◽  
Inertial Microfluidics ◽  
High Efficient ◽  
Integrated Device ◽  
Plasma Extraction

Plasma is a host of various analytes such as proteins, metabolites, circulating nucleic acids (CNAs), pathogens. The key process of plasma extraction is to eliminate the contamination from blood cells. Conventional methods, such as centrifugation and membrane filtration, are generally lab-intensive, time consuming and even dangerous. In this study, we report an integrated microfluidic device that combines inertial microfluidics and membrane filter. The integrated microfluidic device was evaluated by the diluted (x1/10, x1/20) whole blood, and the quality of the extracted blood plasma was tested. It was found that quality of extracted blood plasma from integrated device was equivalent to that obtained by the centrifugation. This study demonstrates a significant progress towards the practical application of inertial microfluidics with membrane filter for high-throughput and high efficient blood plasma extraction.

scholarly journals Fast and Easy Phage-Tagging and Live/Dead Analysis for the Rapid Monitoring of Bacteriophage Infection

2020 ◽  
Vol 11 ◽  
Author(s):  
Hui Zhi Low ◽  
Christina Böhnlein ◽  
Sabrina Sprotte ◽  
Natalia Wagner ◽  
Gregor Fiedler ◽  
...  
Keyword(s):  
High Throughput ◽  
Fluorescent Proteins ◽  
Membrane Filter ◽  
Infection Process ◽  
Resistant Bacteria ◽  
Metabolic Inhibitor ◽  
Host Bacterium ◽  
High Throughput Analysis ◽  
Bacteriophage Infection ◽  
Flow Cytometric

Use of bacteriophages, which are viruses that kill bacteria, for biocontrol of pathogens and antimicrobial resistant bacteria has become increasingly important in recent years. As traditional culture-based methods are laborious and time-consuming, practicable use of bacteriophages will hinge on development of rapid and high throughput methods to analyze, characterize and screen large bacteriophage libraries. We thus established a novel method to fluorescently tag bacteriophages for virus screening and interaction studies, without the need for complicated and laborious purification procedures or genetic engineering of viruses to express fluorescent proteins. Bacteriophage PMBT14 was tagged using DNA dye Syto 13. Simply by using a membrane filter, tagged bacteriophages can be separated from non-sequestered excess dye rapidly, effortlessly, and cheaply. The procedure takes less than 30 min and makes use of simple laboratory consumables that are already commonly used for bacteriophage preparations. As proof of concept, we present here flow cytometric methods to analyze bacteriophage binding, infection and killing that are very accessible for high throughput analysis. We show that the resulting fluorescently tagged bacteriophage can be used to specifically stain its host bacterium Pseudomonas fluorescens DSM 50090. Individual fluorescent bacteriophages, their binding to and initial infection of bacteria could also be observed using confocal microscopy. The infection process was halted by the metabolic inhibitor sodium azide, suggesting a requirement of host metabolic processes for penetration by PMBT14. Flow cytometric live/dead assays was used as a complementary method to determine bacteriophage infection of its host. We made preliminary efforts to adapt the tagging method to two other bacteriophages and discuss potential pitfalls and solutions in the use of tagged phages. Fluorescent phage tagging has previously been demonstrated to facilitate analysis of bacteriophage–host interactions. The method adopted in this study makes it fast, easy as well as cost effective.

Polymorphic phase transition of a membrane protein - analysis of continuous diffuse electron diffraction intensity

1986 ◽  
Vol 44 ◽  
pp. 166-167
Author(s):  
Douglas L. Dorset ◽  
Andrew K. Massalski
Keyword(s):  
Molecular Sieve ◽  
Three Dimensional ◽  
Pore Geometry ◽  
Two Dimensional ◽  
Diffraction Intensity ◽  
Polymorphic Phase Transition ◽  
E Coli ◽  
Crystallographic Study ◽  
Hexagonal Form ◽  
Polymorphic Forms

Matrix porin, the ompF gene product of E. coli, has been the object of a electron crystallographic study of its pore geometry in an attempt to understand its function as a membrane molecular sieve. Three polymorphic forms have been found for two-dimensional crystals reconstituted in phospholipid, two hexagonal forms with different lipid content and an orthorhombic form coexisting with and similar to the hexagonal form found after lipid loss. In projection these have been shown to retain the same three-fold pore triplet geometry and analyses of three-dimensional data reveal that the small hexagonal and orthorhombic polymorphs have similar structure as well as unit cell spacings.

Fibrillin molecules aggregate to form an extendible beaded structure which is a major component of “Elastin Associated” microfibrils

1989 ◽  
Vol 47 ◽  
pp. 246-247
Author(s):  
Douglas R. Keene ◽  
B. Kerry Maddox ◽  
Marie B. Spurgin ◽  
Lynn Y. Sakai ◽  
Robert W. Glanville
Keyword(s):  
Molecular Sieve ◽  
Room Temperature ◽  
Culture Medium ◽  
Weight Fraction ◽  
Ammonium Bicarbonate ◽  
High Molecular Weight Fraction ◽  
Bicarbonate Buffer ◽  
Human Amnion ◽  
Gold Conjugate ◽  
Ammonium Bicarbonate Buffer

A mouse monoclonal antibody was used to identify beaded aggregates found in guanidine extracts of human amnion as assemblies of fibrillin molecules. These aggregates were also shown to be a major component of extracellular matrix microfibrils. We further demonstrated that the periodicity of these aggregates can be increased when subjected to mechanical stress.Human amnion was extracted with guanidine and the extracted material purified using ion exchange and molecular sieve chromatography. A high molecular weight fraction was precipitated by dialyzing against dilute acetic acid. Part of the precipitate was suspended in 0.2 M ammonium bicarbonate buffer and rotary shadowed. A second portion was resuspended in culture medium containing antibody which recognizes matrix microfibrils, diluted 1:5 in ammonium bicarbonate and reacted for 120 minutes at room temperature. Antibody labeled precipitate was washed by repeated pelleting and resuspension in buffer and then incubated in Janssen GAM 5 nm gold conjugate for 60 minutes at room temperature.

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