Orthogonal Activation of RNA‐Cleaving DNAzymes in Live Cells by Reactive Oxygen Species

2019 ◽  
Vol 58 (40) ◽  
pp. 14167-14172 ◽  
Author(s):  
Lu Xiao ◽  
Chunmei Gu ◽  
Yu Xiang
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Live Cells ◽  
Oxygen Species

Single Plasmonic Nanosprings for Visualizing Reactive-Oxygen-Species-Activated Localized Mechanical Force Transduction in Live Cells

ACS Nano ◽  
2017 ◽  
Vol 11 (1) ◽  
pp. 541-548 ◽  
Author(s):  
Bin Xiong ◽  
Zhenrong Huang ◽  
Hongyan Zou ◽  
Chunyan Qiao ◽  
Yan He ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Mechanical Force ◽  
Live Cells ◽  
Oxygen Species ◽  
Force Transduction

scholarly journals Development of an ultrafast fluorescent probe for specific recognition of hypochlorous acid and its application in live cells

RSC Advances ◽  
2021 ◽  
Vol 11 (40) ◽  
pp. 24669-24672
Author(s):  
Zhencai Xu ◽  
Xiaofeng Wang ◽  
Tingting Duan ◽  
Rong He ◽  
Fangwu Wang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Fluorescent Probe ◽  
Hypochlorous Acid ◽  
Reactive Oxygen ◽  
Inflammatory Cells ◽  
Live Cells ◽  
Oxygen Species ◽  
Specific Recognition

Hypochlorous acid (HOCl), a highly potent oxidant of reactive oxygen species, plays critical roles in many physiological and pathological processes. In this work, we designed a simple coumarin-based fluorescent probe, Cou–HOCl, for detecting HOCl in inflammatory cells.

Orthogonal Activation of RNA‐Cleaving DNAzymes in Live Cells by Reactive Oxygen Species

2019 ◽  
Vol 131 (40) ◽  
pp. 14305-14310 ◽  
Author(s):  
Lu Xiao ◽  
Chunmei Gu ◽  
Yu Xiang
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Live Cells ◽  
Oxygen Species

scholarly journals Exogenously Applied Polyamines Reduce Reactive Oxygen Species, Enhancing Cell Division and the Shoot Regeneration from Brassica oleracea L. var. capitata Protoplasts

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 735
Author(s):  
Agnieszka Kiełkowska ◽  
Adela Adamus
Keyword(s):  
Reactive Oxygen Species ◽  
Brassica Oleracea ◽  
Mitotic Activity ◽  
Stress Responses ◽  
Cultured Cells ◽  
Reactive Oxygen ◽  
Cellular Growth ◽  
Live Cells ◽  
Oxygen Species ◽  
Brassica Oleracea L

Polyamines (PAs) are organic molecules that are found in plants and animals. In plants, they are involved in the regulation of cellular growth, apoptosis, rooting, flower development, and stress responses. The effect of exogenously applied polyamines on the development of Brassica oleracea L. var. capitata protoplast cultures was studied. Protoplasts were isolated from hypocotyls of 2-week-old seedlings of three accessions and they were cultured in liquid media supplemented with putrescine (Put), spermidine (Spd), and spermine (Spm) at concentrations of 0 (control), 10, 20, and 40 µM. In the very early culture (24 and 48 h), cellular reactive oxygen species levels (ROS) in live cells were monitored using a fluorescent probe. The Put- and Spd-treated protoplasts exhibited lower fluorescence intensities, which corresponded to lower ROS accumulation as compared to the PA-free control. The protoplast viability was affected by the type of polyamine applied rather than its concentration. Put and Spd had a beneficial effect on the mitotic activity of the cultured cells, which was observed in all tested accessions. The highest frequency of shoot organogenesis (21%) was obtained from microcalli derived from the protoplasts cultured on the medium supplemented with 10 µM Put. Analysis of the ploidy level of the regenerants showed that the vast majority were diploids. Our results demonstrated that exogenously applied PAs maintained the viability of B. oleracea L. var. capitata protoplasts by alleviating oxidative stress and stimulating mitotic activity, which further affected the plant regeneration process.

A two-photon fluorescent probe for ratiometric imaging of endogenous hypochlorous acid in live cells and tissues

2017 ◽  
Vol 53 (78) ◽  
pp. 10800-10803 ◽  
Author(s):  
Yong Woong Jun ◽  
Sourav Sarkar ◽  
Subhankar Singha ◽  
Ye Jin Reo ◽  
Hye Rim Kim ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Fluorescent Probe ◽  
Hypochlorous Acid ◽  
Reactive Oxygen ◽  
Live Cells ◽  
Oxygen Species ◽  
Two Photon ◽  
Ratiometric Imaging ◽  
In Cells

A fluorescent probe enables two-photon ratiometric imaging of endogenous hypochlorous acid, a reactive oxygen species, in cells and tissues.

scholarly journals Development of Fluorogenic Antioxidants to Monitor Reactive Oxygen Species in the Lipid Membrane of Live Cells.

2014 ◽  
Vol 20 (S3) ◽  
pp. 1356-1357
Author(s):  
Katerina Krumova ◽  
Lana E. Greene ◽  
Robert Godin ◽  
Richard Lincoln ◽  
Gonzalo Cosa
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Membrane ◽  
Reactive Oxygen ◽  
Live Cells ◽  
Oxygen Species
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