scholarly journals Switchable Solvatochromic Probes for Live‐Cell Super‐resolution Imaging of Plasma Membrane Organization

2019 ◽  
Vol 58 (42) ◽  
pp. 14920-14924 ◽  
Author(s):  
Dmytro I. Danylchuk ◽  
Seonah Moon ◽  
Ke Xu ◽  
Andrey S. Klymchenko
Keyword(s):  
Plasma Membrane ◽  
Super Resolution ◽  
Live Cell ◽  
Membrane Organization ◽  
Solvatochromic Probes ◽  
Resolution Imaging

scholarly journals Switchable Solvatochromic Probes for Live‐Cell Super‐resolution Imaging of Plasma Membrane Organization

2019 ◽  
Vol 131 (42) ◽  
pp. 15062-15066 ◽  
Author(s):  
Dmytro I. Danylchuk ◽  
Seonah Moon ◽  
Ke Xu ◽  
Andrey S. Klymchenko
Keyword(s):  
Plasma Membrane ◽  
Super Resolution ◽  
Live Cell ◽  
Membrane Organization ◽  
Solvatochromic Probes ◽  
Resolution Imaging

scholarly journals Local palmitoylation cycles define activity-regulated postsynaptic subdomains

2013 ◽  
Vol 202 (1) ◽  
pp. 145-161 ◽  
Author(s):  
Yuko Fukata ◽  
Ariane Dimitrov ◽  
Gaelle Boncompain ◽  
Ole Vielemeyer ◽  
Franck Perez ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Dendritic Spines ◽  
Recombinant Antibody ◽  
Super Resolution ◽  
Live Cell ◽  
Synaptic Activity ◽  
Membrane Targeting ◽  
Resolution Imaging

Distinct PSD-95 clusters are primary landmarks of postsynaptic densities (PSDs), which are specialized membrane regions for synapses. However, the mechanism that defines the locations of PSD-95 clusters and whether or how they are reorganized inside individual dendritic spines remains controversial. Because palmitoylation regulates PSD-95 membrane targeting, we combined a conformation-specific recombinant antibody against palmitoylated PSD-95 with live-cell super-resolution imaging and discovered subsynaptic nanodomains composed of palmitoylated PSD-95 that serve as elementary units of the PSD. PSD-95 in nanodomains underwent continuous de/repalmitoylation cycles driven by local palmitoylating activity, ensuring the maintenance of compartmentalized PSD-95 clusters within individual spines. Plasma membrane targeting of DHHC2 palmitoyltransferase rapidly recruited PSD-95 to the plasma membrane and proved essential for postsynaptic nanodomain formation. Furthermore, changes in synaptic activity rapidly reorganized PSD-95 nano-architecture through plasma membrane–inserted DHHC2. Thus, the first genetically encoded antibody sensitive to palmitoylation reveals an instructive role of local palmitoylation machinery in creating activity-responsive PSD-95 nanodomains, contributing to the PSD (re)organization.

Instant Live-Cell Super-Resolution Imaging of Cellular Structures by Nanoinjection of Fluorescent Probes

Nano Letters ◽  
2015 ◽  
Vol 15 (2) ◽  
pp. 1374-1381 ◽  
Author(s):  
Simon Hennig ◽  
Sebastian van de Linde ◽  
Martina Lummer ◽  
Matthias Simonis ◽  
Thomas Huser ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Super Resolution ◽  
Live Cell ◽  
Cellular Structures ◽  
Resolution Imaging

Live-Cell Super-Resolution Imaging with Synthetic Fluorophores

2012 ◽  
Vol 63 (1) ◽  
pp. 519-540 ◽  
Author(s):  
Sebastian van de Linde ◽  
Mike Heilemann ◽  
Markus Sauer
Keyword(s):  
Super Resolution ◽  
Live Cell ◽  
Resolution Imaging

scholarly journals Super-Resolution Imaging of Plasma Membrane Lesions Inflicted by 405-nm Laser Light

2016 ◽  
Vol 110 (3) ◽  
pp. 488a
Author(s):  
Lu Zhou ◽  
Volker Middel ◽  
G. Ulrich Nienhaus ◽  
Uwe Uwe Strähle
Keyword(s):  
Plasma Membrane ◽  
Laser Light ◽  
Super Resolution ◽  
Resolution Imaging

Live cell super resolution imaging by radial fluctuations using fluorogen binding tags

Nanoscale ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 3626-3632 ◽  
Author(s):  
Muthukumaran Venkatachalapathy ◽  
Vivek Belapurkar ◽  
Mini Jose ◽  
Arnaud Gautier ◽  
Deepak Nair
Keyword(s):  
Super Resolution ◽  
Time Lapse ◽  
Live Cell ◽  
Resolution Imaging

Combination of SRRF and stochastic labeling based on FAST:Fluorogen complexes to achieve super-resolution in 2D, 3D and in time-lapse.

Sign in / Sign up

Export Citation Format

Share Document