An Unexpected Role of Hyaluronic Acid in Trafficking siRNA Across the Cellular Barrier: The First Biomimetic, Anionic, Non‐Viral Transfection Method

2019 ◽  
Vol 58 (9) ◽  
pp. 2815-2819 ◽  
Author(s):  
Maruthibabu Paidikondala ◽  
Vignesh Kumar Rangasami ◽  
Ganesh N. Nawale ◽  
Tommaso Casalini ◽  
Giuseppe Perale ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Transfection Method ◽  
Viral Transfection

An Unexpected Role of Hyaluronic Acid in Trafficking siRNA Across the Cellular Barrier: The First Biomimetic, Anionic, Non‐Viral Transfection Method

2019 ◽  
Vol 131 (9) ◽  
pp. 2841-2845
Author(s):  
Maruthibabu Paidikondala ◽  
Vignesh Kumar Rangasami ◽  
Ganesh N. Nawale ◽  
Tommaso Casalini ◽  
Giuseppe Perale ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Transfection Method ◽  
Viral Transfection

scholarly journals Secreted KIAA1199 promotes the progression of rheumatoid arthritis by mediating hyaluronic acid degradation in an ANXA1-dependent manner

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Wei Zhang ◽  
Guoyu Yin ◽  
Heping Zhao ◽  
Hanzhi Ling ◽  
Zhen Xie ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Hyaluronic Acid ◽  
Dependent Manner ◽  
Collagen Induced Arthritis ◽  
Intracellular Degradation ◽  
Main Pathway ◽  
First Time

AbstractIn inflamed joints, enhanced hyaluronic acid (HA) degradation is closely related to the pathogenesis of rheumatoid arthritis (RA). KIAA1199 has been identified as a hyaladherin that mediates the intracellular degradation of HA, but its extracellular function remains unclear. In this study, we found that the serum and synovial levels of secreted KIAA1199 (sKIAA1199) and low-molecular-weight HA (LMW-HA, MW < 100 kDa) in RA patients were significantly increased, and the positive correlation between them was shown for the first time. Of note, treatment with anti-KIAA1199 mAb effectively alleviated the severity of arthritis and reduced serum LMW-HA levels and cytokine secretion in collagen-induced arthritis (CIA) mice. In vitro, sKIAA1199 was shown to mediate exogenous HA degradation by attaching to the cell membrane of RA fibroblast-like synoviosytes (RA FLS). Furthermore, the HA-degrading activity of sKIAA1199 depended largely on its adhesion to the membrane, which was achieved by its G8 domain binding to ANXA1. In vivo, kiaa1199-KO mice exhibited greater resistance to collagen-induced arthritis. Interestingly, this resistance could be partially reversed by intra-articular injection of vectors encoding full-length KIAA1199 instead of G8-deleted KIAA119 mutant, which further confirmed the indispensable role of G8 domain in KIAA1199 involvement in RA pathological processes. Mechanically, the activation of NF-κB by interleukin-6 (IL-6) through PI3K/Akt signaling is suggested to be the main pathway to induce KIAA1199 expression in RA FLS. In conclusion, our study supported the contribution of sKIAA1199 to RA pathogenesis, providing a new therapeutic target for RA by blocking sKIAA1199-mediated HA degradation.

scholarly journals The Role of Hyaluronic Acid in Cartilage Boundary Lubrication

Cells ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 1606 ◽  
Author(s):  
Weifeng Lin ◽  
Zhang Liu ◽  
Nir Kampf ◽  
Jacob Klein
Keyword(s):  
Hyaluronic Acid ◽  
Synovial Fluid ◽  
Surface Force ◽  
Force Balance ◽  
New Paradigm ◽  
Low Friction ◽  
Cartilage Surface ◽  
Boundary Lubricant ◽  
Lipid Layers

Hydration lubrication has emerged as a new paradigm for lubrication in aqueous and biological media, accounting especially for the extremely low friction (friction coefficients down to 0.001) of articular cartilage lubrication in joints. Among the ensemble of molecules acting in the joint, phosphatidylcholine (PC) lipids have been proposed as the key molecules forming, in a complex with other molecules including hyaluronic acid (HA), a robust layer on the outer surface of the cartilage. HA, ubiquitous in synovial joints, is not in itself a good boundary lubricant, but binds the PC lipids at the cartilage surface; these, in turn, massively reduce the friction via hydration lubrication at their exposed, highly hydrated phosphocholine headgroups. An important unresolved issue in this scenario is why the free HA molecules in the synovial fluid do not suppress the lubricity by adsorbing simultaneously to the opposing lipid layers, i.e., forming an adhesive, dissipative bridge between them, as they slide past each other during joint articulation. To address this question, we directly examined the friction between two hydrogenated soy PC (HSPC) lipid layers (in the form of liposomes) immersed in HA solution or two palmitoyl–oleoyl PC (POPC) lipid layers across HA–POPC solution using a surface force balance (SFB). The results show, clearly and surprisingly, that HA addition does not affect the outstanding lubrication provided by the PC lipid layers. A possible mechanism indicated by our data that may account for this is that multiple lipid layers form on each cartilage surface, so that the slip plane may move from the midplane between the opposing surfaces, which is bridged by the HA, to an HA-free interface within a multilayer, where hydration lubrication is freely active. Another possibility suggested by our model experiments is that lipids in synovial fluid may complex with HA, thereby inhibiting the HA molecules from adhering to the lipids on the cartilage surfaces.

scholarly journals Role of Hyaluronic Acid in the Homeostasis and Therapeutics of Temporomandibular Joint Osteoarthritis

2017 ◽  
Vol 35 (3) ◽  
pp. 870-876 ◽  
Author(s):  
Veronica Iturriaga ◽  
Bélgica Vásquez ◽  
Carlos Manterola ◽  
Mariano del Sol
Keyword(s):  
Hyaluronic Acid ◽  
Temporomandibular Joint ◽  
Temporomandibular Joint Osteoarthritis

Role of a hyaluronic-acid derivative in preventing surgical adhesions and abscesses related to dropped bile and gallstones in an experimental model

2004 ◽  
Vol 188 (3) ◽  
pp. 288-293 ◽  
Author(s):  
Faruk O. Aytekin ◽  
Koray Tekin ◽  
Burhan Kabay ◽  
Ergun Erdem ◽  
Halil Erbis ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Experimental Model ◽  
Acid Derivative ◽  
Surgical Adhesions

The role of hyaluronic acid inclusion on the energetics of encapsulation and release of a protein molecule from chitosan-based nanoparticles

2016 ◽  
Vol 141 ◽  
pp. 223-232 ◽  
Author(s):  
Sonia Al-Qadi ◽  
Manuel Alatorre-Meda ◽  
Manuel Martin-Pastor ◽  
Pablo Taboada ◽  
Carmen Remuñán-López
Keyword(s):  
Hyaluronic Acid ◽  
Protein Molecule
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