scholarly journals Perfluorocarbon‐Based 19 F MRI Nanoprobes for In Vivo Multicolor Imaging

2018 ◽  
Vol 57 (51) ◽  
pp. 16742-16747 ◽  
Author(s):  
Kazuki Akazawa ◽  
Fuminori Sugihara ◽  
Tatsuya Nakamura ◽  
Hisashi Matsushita ◽  
Hiroaki Mukai ◽  
...  
Keyword(s):  
Multicolor Imaging

scholarly journals Perfluorocarbon‐Based 19 F MRI Nanoprobes for In Vivo Multicolor Imaging

2018 ◽  
Vol 130 (51) ◽  
pp. 16984-16989
Author(s):  
Kazuki Akazawa ◽  
Fuminori Sugihara ◽  
Tatsuya Nakamura ◽  
Hisashi Matsushita ◽  
Hiroaki Mukai ◽  
...  
Keyword(s):  
Multicolor Imaging

Trichromatic-emission and dual-ratio semiconducting polymer dots as fluorescent probe for simultaneous quantification of Cu2+ and pH in vitro and in vivo

2020 ◽  
Vol 56 (61) ◽  
pp. 8647-8650
Author(s):  
Qiang Zhang ◽  
Junyong Sun ◽  
Rongchao Zhang ◽  
Xueli Chen ◽  
Ningning Chen ◽  
...  
Keyword(s):  
Hela Cells ◽  
Simultaneous Detection ◽  
Blue Color ◽  
Semiconducting Polymer ◽  
Simultaneous Quantification ◽  
Polymer Dots ◽  
Multicolor Imaging ◽  
Semiconducting Polymer Dots

Polymer dots emitting in the red, green and blue color regions, have been successfully applied as lysosome-targeting nanoprobes for the simultaneous detection and multicolor imaging of pH and Cu2+ in HeLa cells and zebrafish.

Autofluorescence-free in vivo multicolor imaging using upconversion fluoride nanocrystals

2009 ◽  
Vol 25 (4) ◽  
pp. 479-484 ◽  
Author(s):  
Zhen Tian ◽  
Guanying Chen ◽  
Xiang Li ◽  
Huijuan Liang ◽  
Yuanshi Li ◽  
...  
Keyword(s):  
Multicolor Imaging

Water-soluble fluorescent unimolecular micelles: ultra-small size, tunable fluorescence emission from the visible to NIR region and enhanced biocompatibility for in vitro and in vivo bioimaging

2018 ◽  
Vol 54 (49) ◽  
pp. 6252-6255 ◽  
Author(s):  
Xiaoqian Ma ◽  
Xiaoxiao Shi ◽  
Shuang Bai ◽  
Jianjian Zhang ◽  
Meili Hou ◽  
...  
Keyword(s):  
Fluorescence Emission ◽  
Water Soluble ◽  
Multicolor Imaging

Water-soluble fluorescent unimolecular micelles with ultra-small size and various fluorescence emission for multicolor imaging.

scholarly journals Body window-enabled in vivo multicolor imaging of transplanted mouse islets expressing an insulin-Timer fusion protein

BioTechniques ◽  
2003 ◽  
Vol 35 (4) ◽  
pp. 718-722 ◽  
Author(s):  
Suzanne Bertera ◽  
Xuehui Geng ◽  
Zakaria Tawadrous ◽  
Rita Bottino ◽  
A.N. Balamurugan ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Multicolor Imaging ◽  
Mouse Islets

scholarly journals In Vivo Multicolor Imaging with Fluorescent Probes Revealed the Dynamics and Function of Osteoclast Proton Pumps

2019 ◽  
Vol 5 (6) ◽  
pp. 1059-1066 ◽  
Author(s):  
Masafumi Minoshima ◽  
Junichi Kikuta ◽  
Yuta Omori ◽  
Shigeto Seno ◽  
Riko Suehara ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Proton Pumps ◽  
Multicolor Imaging ◽  
Dynamics And Function ◽  
And Function

scholarly journals Fibered confocal spectroscopy and multicolor imaging system for in vivo fluorescence analysis

2007 ◽  
Vol 15 (7) ◽  
pp. 4008 ◽  
Author(s):  
Florence Jean ◽  
Genevieve Bourg-Heckly ◽  
Bertrand Viellerobe
Keyword(s):  
Imaging System ◽  
Fluorescence Analysis ◽  
In Vivo Fluorescence ◽  
Multicolor Imaging ◽  
Confocal Spectroscopy

scholarly journals Rapid Directed Molecular Evolution of Fluorescent Proteins in Mammalian Cells

2021 ◽  
Author(s):  
Kiryl D Piatkevich ◽  
Siranush Babakhanova ◽  
Erica Jung ◽  
Kazuhiko Namikawa ◽  
Hanbin ZHANG ◽  
...  
Keyword(s):  
Molecular Evolution ◽  
Mammalian Cells ◽  
High Performance ◽  
Near Infrared ◽  
Fluorescent Proteins ◽  
Fluorescent Labeling ◽  
Model Organisms ◽  
Multicolor Imaging ◽  
Directed Molecular Evolution

In vivo imaging of model organisms is heavily reliant on fluorescent proteins with high intracellular brightness. Here we describe a practical method for rapid optimization of fluorescent proteins via directed molecular evolution in cultured mammalian cells. Using this method, we were able to perform screening of large gene libraries containing up to 2x107 independent random genes of fluorescent proteins expressed in HEK cells completing one iteration directed evolution in a course of ~8 days. We employed this approach to develop a set of green and near-infrared fluorescent proteins with enhanced intracellular brightness. The developed near-infrared fluorescent proteins demonstrated high performance for fluorescent labeling of neurons in culture and in vivo in model organisms such as C.elegans, Drosophila, zebrafish, and mice. Spectral properties of the optimized near-infrared fluorescent proteins enabled crosstalk-free multicolor imaging in combination with common green and red fluorescent proteins, as well as dual-color near-infrared fluorescence imaging. The described method has a great potential to be adopted by protein engineers due to its simplicity and practicality. We also believe that the new enhanced fluorescent proteins will find wide application for in vivo multicolor imaging of small model organisms.

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