scholarly journals Red-Shifted Fluorogenic Substrate for Detection of lac Z-Positive Cells in Living Tissue with Single-Cell Resolution

2018 ◽  
Vol 57 (48) ◽  
pp. 15702-15706 ◽  
Author(s):  
Hiroki Ito ◽  
Yu Kawamata ◽  
Mako Kamiya ◽  
Kayoko Tsuda-Sakurai ◽  
Shinji Tanaka ◽  
...  
Keyword(s):  
Single Cell ◽  
Living Tissue ◽  
Fluorogenic Substrate ◽  
Lac Z

scholarly journals Red-Shifted Fluorogenic Substrate for Detection of lac Z-Positive Cells in Living Tissue with Single-Cell Resolution

2018 ◽  
Vol 130 (48) ◽  
pp. 15928-15932 ◽  
Author(s):  
Hiroki Ito ◽  
Yu Kawamata ◽  
Mako Kamiya ◽  
Kayoko Tsuda-Sakurai ◽  
Shinji Tanaka ◽  
...  
Keyword(s):  
Single Cell ◽  
Living Tissue ◽  
Fluorogenic Substrate ◽  
Lac Z

Detection ofLacZ-Positive Cells in Living Tissue with Single-Cell Resolution

2016 ◽  
Vol 128 (33) ◽  
pp. 9772-9776 ◽  
Author(s):  
Tomohiro Doura ◽  
Mako Kamiya ◽  
Fumiaki Obata ◽  
Yoshifumi Yamaguchi ◽  
Takeshi Y. Hiyama ◽  
...  
Keyword(s):  
Single Cell ◽  
Living Tissue

Detection ofLacZ-Positive Cells in Living Tissue with Single-Cell Resolution

2016 ◽  
Vol 55 (33) ◽  
pp. 9620-9624 ◽  
Author(s):  
Tomohiro Doura ◽  
Mako Kamiya ◽  
Fumiaki Obata ◽  
Yoshifumi Yamaguchi ◽  
Takeshi Y. Hiyama ◽  
...  
Keyword(s):  
Single Cell ◽  
Living Tissue

Electron microscopy of keratinocytes cultured on a collagen substrate used as an allograft for the treatment of chronic wounds

1992 ◽  
Vol 50 (2) ◽  
pp. 1104-1105
Author(s):  
Debby A. Jennings ◽  
Michael J. Morykwas ◽  
Louis C. Argenta
Keyword(s):  
Electron Microscopy ◽  
Cell Suspension ◽  
Single Cell ◽  
Chronic Wounds ◽  
Mechanical Stability ◽  
Uv Light ◽  
Type I ◽  
Single Cell Suspension ◽  
Collagen Substrate ◽  
Dermal Collagen

Grafts of cultured allogenic or autogenic keratlnocytes have proven to be an effective treatment of chronic wounds and burns. This study utilized a collagen substrate for keratinocyte and fibroblast attachment. The substrate provided mechanical stability and augmented graft manipulation onto the wound bed. Graft integrity was confirmed by light and transmission electron microscopy.Bovine Type I dermal collagen sheets (100 μm thick) were crosslinked with 254 nm UV light (13.5 Joules/cm2) to improve mechanical properties and reduce degradation. A single cell suspension of third passage neonatal foreskin fibroblasts were plated onto the collagen. Five days later, a single cell suspension of first passage neonatal foreskin keratinocytes were plated on the opposite side of the collagen. The grafts were cultured for one month.The grafts were fixed in phosphate buffered 4% formaldehyde/1% glutaraldehyde for 24 hours. Graft pieces were then washed in 0.13 M phosphate buffer, post-fixed in 1% osmium tetroxide, dehydrated, and embedded in Polybed 812.

Author response for "Immunocyte single cell analysis of vaccine‐induced narcolepsy"

2020 ◽  
Author(s):  
Alexander Lind ◽  
Falastin Salami ◽  
Anne‐Marie Landtblom ◽  
Lars Palm ◽  
Åke Lernmark ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Author Response ◽  
Cell Analysis
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