scholarly journals Acute Modulation of Mycobacterial Cell Envelope Biogenesis by Front-Line Tuberculosis Drugs

2018 ◽  
Vol 57 (19) ◽  
pp. 5267-5272 ◽  
Author(s):  
Frances P. Rodriguez-Rivera ◽  
Xiaoxue Zhou ◽  
Julie A. Theriot ◽  
Carolyn R. Bertozzi
Keyword(s):  
Cell Envelope ◽  
Front Line ◽  
Mycobacterial Cell

Acute Modulation of Mycobacterial Cell Envelope Biogenesis by Front-Line Tuberculosis Drugs

2018 ◽  
Vol 130 (19) ◽  
pp. 5365-5370 ◽  
Author(s):  
Frances P. Rodriguez-Rivera ◽  
Xiaoxue Zhou ◽  
Julie A. Theriot ◽  
Carolyn R. Bertozzi
Keyword(s):  
Cell Envelope ◽  
Front Line ◽  
Mycobacterial Cell

scholarly journals Antibiotic action revealed by real-time imaging of the mycobacterial membrane

2022 ◽  
Author(s):  
Michael G. Wuo ◽  
Charles L Dulberger ◽  
Robert A. Brown ◽  
Alexander Sturm ◽  
Eveline Ultee ◽  
...  
Keyword(s):  
Real Time ◽  
Immune Modulation ◽  
Cell Envelope ◽  
Membrane Vesicles ◽  
Time Lapse ◽  
Outer Membrane Vesicles ◽  
Imaging Data ◽  
Minimal Cell ◽  
Mycobacterial Cell ◽  
Cellular Phenotypes

The current understanding of mycobacterial cell envelope remodeling in response to antibiotics is limited. Chemical tools that report on phenotypic changes with minimal cell wall perturbation are critical to gaining insight into this time-dependent phenomenon. Herein we describe a fluorogenic chemical probe that reports on mycobacterial cell envelope assembly in real time. We used time-lapse microscopy to reveal distinct spatial and temporal changes in the mycobacterial membrane upon treatment with frontline antibiotics. Differential antibiotic treatment elicited unique cellular phenotypes, providing a platform for monitoring cell envelope construction and remodeling responses simultaneously. Analysis of the imaging data indicates a role for antibiotic-derived outer membrane vesicles in immune modulation.

scholarly journals Trehalose recycling promotes energy-efficient mycomembrane reorganization in nutrient-limited mycobacteria

2019 ◽  
Author(s):  
Amol Arunrao Pohane ◽  
Caleb R. Carr ◽  
Jaishree Garhyan ◽  
Benjamin M. Swarts ◽  
M. Sloan Siegrist
Keyword(s):  
Energy Efficient ◽  
De Novo ◽  
Cell Envelope ◽  
Bacterial Pathogens ◽  
Atp Depletion ◽  
Redox Stress ◽  
Mycobacterial Cell ◽  
Resource Limited ◽  
Trehalose Synthesis ◽  
Long Chain

AbstractThe mycomembrane layer of the mycobacterial cell envelope is a barrier to environmental, immune and antibiotic insults. We find that there is mycomembrane remodeling along the periphery of nutrient-starved, non-replicating mycobacterial cells. Remodeling is supported by recycling of trehalose, a non-mammalian disaccharide that shuttles long-chain mycolate lipids to the mycomembrane. In the absence of trehalose recycling, mycomembrane synthesis continues but mycobacteria experience ATP depletion, enhanced respiration and redox stress. Redox stress from depletion of the trehalose pool is suppressed in a mutant that lacks the OtsAB de novo trehalose synthesis pathway. Our data suggest that trehalose recycling alleviates the energetic burden of mycomembrane remodeling. Loss of trehalose salvage is known to attenuate M. tuberculosis during infection and render the bacterium more susceptible to a variety of drugs. Recycling pathways are emerging targets for sensitizing resource-limited bacterial pathogens to host and antibiotic stress.

Transporters Involved in the Biogenesis and Functionalization of the Mycobacterial Cell Envelope

2020 ◽  
Author(s):  
Mary Jackson ◽  
Casey M. Stevens ◽  
Lei Zhang ◽  
Helen I. Zgurskaya ◽  
Michael Niederweis
Keyword(s):  
Cell Envelope ◽  
Mycobacterial Cell

Tuberculosis vaccine candidates based on mycobacterial cell envelope components

Tuberculosis ◽  
2019 ◽  
Vol 115 ◽  
pp. 26-41 ◽  
Author(s):  
M.E. Sarmiento ◽  
N. Alvarez ◽  
K.L. Chin ◽  
F. Bigi ◽  
Y. Tirado ◽  
...  
Keyword(s):  
Cell Envelope ◽  
Vaccine Candidates ◽  
Mycobacterial Cell

scholarly journals MmpL3 is a lipid transporter that binds trehalose monomycolate and phosphatidylethanolamine

2019 ◽  
Vol 116 (23) ◽  
pp. 11241-11246 ◽  
Author(s):  
Chih-Chia Su ◽  
Philip A. Klenotic ◽  
Jani Reddy Bolla ◽  
Georgiana E. Purdy ◽  
Carol V. Robinson ◽  
...  
Keyword(s):  
Cell Wall ◽  
Cell Envelope ◽  
Native Mass Spectrometry ◽  
Mycolic Acids ◽  
Mycobacterial Cell ◽  
Exact Function ◽  
Species Specific ◽  
Mycobacterial Cell Wall ◽  
Trehalose Monomycolate ◽  
Lipid Transporter

The cell envelope ofMycobacterium tuberculosisis notable for the abundance of mycolic acids (MAs), essential to mycobacterial viability, and of other species-specific lipids. The mycobacterial cell envelope is extremely hydrophobic, which contributes to virulence and antibiotic resistance. However, exactly how fatty acids and lipidic elements are transported across the cell envelope for cell-wall biosynthesis is unclear. Mycobacterial membrane protein Large 3 (MmpL3) is essential and required for transport of trehalose monomycolates (TMMs), precursors of MA-containing trehalose dimycolates (TDM) and mycolyl arabinogalactan peptidoglycan, but the exact function of MmpL3 remains elusive. Here, we report a crystal structure ofMycobacterium smegmatisMmpL3 at a resolution of 2.59 Å, revealing a monomeric molecule that is structurally distinct from all known bacterial membrane proteins. A previously unknown MmpL3 ligand, phosphatidylethanolamine (PE), was discovered inside this transporter. We also show, via native mass spectrometry, that MmpL3 specifically binds both TMM and PE, but not TDM, in the micromolar range. These observations provide insight into the function of MmpL3 and suggest a possible role for this protein in shuttling a variety of lipids to strengthen the mycobacterial cell wall.

scholarly journals Chemical and ultrastructural investigations ofMycobacterium bovisBCG: implications for the molecular structure of the mycobacterial cell envelope

1996 ◽  
Vol 15 (4) ◽  
pp. 213-222 ◽  
Author(s):  
Melvin E. Klegerman ◽  
Priscilla O. Devadoss ◽  
José L. Garrido ◽  
Hector R. Reyes ◽  
Michael J. Groves
Keyword(s):  
Molecular Structure ◽  
Cell Envelope ◽  
Mycobacterial Cell

scholarly journals Dissecting the mycobacterial cell envelope and defining the composition of the native mycomembrane

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Laura Chiaradia ◽  
Cyril Lefebvre ◽  
Julien Parra ◽  
Julien Marcoux ◽  
Odile Burlet-Schiltz ◽  
...  
Keyword(s):  
Cell Envelope ◽  
Mycobacterial Cell
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