A General Mechanism of Photoconversion of Green-to-Red Fluorescent Proteins Based on Blue and Infrared Light Reduces Phototoxicity in Live-Cell Single-Molecule Imaging

2017 ◽  
Vol 56 (38) ◽  
pp. 11634-11639 ◽  
Author(s):  
Bartosz Turkowyd ◽  
Alexander Balinovic ◽  
David Virant ◽  
Haruko G. Gölz Carnero ◽  
Fabienne Caldana ◽  
...  
Keyword(s):  
Single Molecule ◽  
Fluorescent Proteins ◽  
Live Cell ◽  
Infrared Light ◽  
General Mechanism ◽  
Single Molecule Imaging ◽  
Red Fluorescent Proteins

scholarly journals Phase-Separated Transcriptional Condensates Accelerate Target-Search Process Revealed by Live-Cell Single-Molecule Imaging

Cell Reports ◽  
2020 ◽  
Vol 33 (2) ◽  
pp. 108248 ◽  
Author(s):  
Samantha Kent ◽  
Kyle Brown ◽  
Chou-hsun Yang ◽  
Njood Alsaihati ◽  
Christina Tian ◽  
...  
Keyword(s):  
Single Molecule ◽  
Live Cell ◽  
Search Process ◽  
Single Molecule Imaging ◽  
Target Search

scholarly journals tdLanYFP, a yellow, bright, photostable and pH insensitive fluorescent protein for live cell imaging and FRET-based sensing strategies

2021 ◽  
Author(s):  
Y. Bousmah ◽  
H. Valenta ◽  
G. Bertolin ◽  
U. Singh ◽  
V. Nicolas ◽  
...  
Keyword(s):  
Single Molecule ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Yellow Fluorescent Protein ◽  
Resonance Energy ◽  
Live Cell ◽  
Live Cells

AbstractYellow fluorescent proteins (YFP) are widely used as optical reporters in Förster Resonance Energy Transfer (FRET) based biosensors. Although great improvements have been done, the sensitivity of the biosensors is still limited by the low photostability and the poor fluorescence performances of YFPs at acidic pHs. In fact, today, there is no yellow variant derived from the EYFP with a pK1/2 below ∼5.5. Here, we characterize a new yellow fluorescent protein, tdLanYFP, derived from the tetrameric protein from the cephalochordate B. lanceolatum, LanYFP. With a quantum yield of 0.92 and an extinction coefficient of 133 000 mol−1.L.cm−1, it is, to our knowledge, the brightest dimeric fluorescent protein available, and brighter than most of the monomeric YFPs. Contrasting with EYFP and its derivatives, tdLanYFP has a very high photostability in vitro and preserves this property in live cells. As a consequence, tdLanYFP allows the imaging of cellular structures with sub-diffraction resolution with STED nanoscopy. We also demonstrate that the combination of high brightness and strong photostability is compatible with the use of spectro-microscopies in single molecule regimes. Its very low pK1/2 of 3.9 makes tdLanYFP an excellent tag even at acidic pHs. Finally, we show that tdLanYFP can be a FRET partner either as donor or acceptor in different biosensing modalities. Altogether, these assets make tdLanYFPa very attractive yellow fluorescent protein for long-term or single-molecule live-cell imaging that is also suitable for FRET experiment including at acidic pH.

scholarly journals Live-cell single-molecule imaging reveals clathrin and caveolin-1 dependent docking of SMAD4 at the cell membrane

FEBS Letters ◽  
2013 ◽  
Vol 587 (24) ◽  
pp. 3912-3920 ◽  
Author(s):  
Yong Yang ◽  
Joy Wolfram ◽  
Jianliang Shen ◽  
Yuliang Zhao ◽  
Xiaohong Fang ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Single Molecule ◽  
Live Cell ◽  
Single Molecule Imaging ◽  
Caveolin 1

scholarly journals Electronic tuning of self-healing fluorophores for live-cell and single-molecule imaging

2017 ◽  
Vol 8 (1) ◽  
pp. 755-762 ◽  
Author(s):  
Qinsi Zheng ◽  
Steffen Jockusch ◽  
Zhou Zhou ◽  
Roger B. Altman ◽  
Hong Zhao ◽  
...  
Keyword(s):  
Single Molecule ◽  
Live Cell ◽  
Single Molecule Imaging ◽  
Self Healing ◽  
Electronic Tuning ◽  
Mechanistic Investigation

Mechanistic investigation of self-healing fluorophores leads to a general approach for highly photostable fluorophores across the spectrum.

scholarly journals Live-Cell Single-Molecule Imaging of Endogenous MRNA in Stress Granules

2016 ◽  
Vol 110 (3) ◽  
pp. 647a
Author(s):  
Ko Sugawara ◽  
Kohki Okabe ◽  
Takashi Funatsu
Keyword(s):  
Single Molecule ◽  
Stress Granules ◽  
Live Cell ◽  
Single Molecule Imaging
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