scholarly journals Control of Nanoparticle Release Kinetics from 3D Printed Hydrogel Scaffolds

2017 ◽  
Vol 56 (16) ◽  
pp. 4623-4628 ◽  
Author(s):  
Bernhard Baumann ◽  
Tomasz Jungst ◽  
Simone Stichler ◽  
Susanne Feineis ◽  
Oliver Wiltschka ◽  
...  
Keyword(s):  
Release Kinetics ◽  
Hydrogel Scaffolds ◽  
3D Printed ◽  
Nanoparticle Release

A 3D-printed Microfluidic System for Automated and Near Real-time Quantitation of Biofilm-induced indole

2019 ◽  
Author(s):  
Giraso Kabandana ◽  
Curtis G. Jones ◽  
Sahra Khan Sharifi ◽  
Chengpeng Chen
Keyword(s):  
Real Time ◽  
Release Kinetics ◽  
Microfluidic System ◽  
3D Printed

We developed a novel microfluidic system that enables automated and near real-time quantitation of indole release kinetics from biofilms.

Preparation of 3D Printed Chitosan/Polyvinyl Alcohol Double Network Hydrogel Scaffolds

2021 ◽  
pp. 2000398
Author(s):  
Fei Liu ◽  
Wenyu Li ◽  
Hongting Liu ◽  
Teng Yuan ◽  
Yu Yang ◽  
...  
Keyword(s):  
Polyvinyl Alcohol ◽  
Double Network ◽  
Hydrogel Scaffolds ◽  
3D Printed

scholarly journals Design and characterization of 3D printed, neomycin-eluting poly-L-lactide mats for wound-healing applications

2021 ◽  
Vol 32 (4) ◽  
Author(s):  
Mahima Singh ◽  
Sriramakamal Jonnalagadda
Keyword(s):  
Release Kinetics ◽  
Biological Properties ◽  
In Vitro Dissolution ◽  
3D Printer ◽  
Topical Antibiotic ◽  
Young’S Moduli ◽  
Base Polymer ◽  
Potential Applications ◽  
3D Printed

AbstractThis study evaluates the suitability of 3D printed biodegradable mats to load and deliver the topical antibiotic, neomycin, for up to 3 weeks in vitro. A 3D printer equipped with a hot melt extruder was used to print bandage-like wound coverings with porous sizes appropriate for cellular attachment and viability. The semicrystalline polyester, poly-l-lactic acid (PLLA) was used as the base polymer, coated (post-printing) with polyethylene glycols (PEGs) of MWs 400 Da, 6 kDa, or 20 kDa to enable manipulation of physicochemical and biological properties to suit intended applications. The mats were further loaded with a topical antibiotic (neomycin sulfate), and cumulative drug-release monitored for 3 weeks in vitro. Microscopic imaging as well as Scanning Electron Microscopy (SEM) studies showed pore dimensions of 100 × 400 µm. These pore dimensions were achieved without compromising mechanical strength; because of the “tough” individual fibers constituting the mat (Young’s Moduli of 50 ± 20 MPa and Elastic Elongation of 10 ± 5%). The in vitro dissolution study showed first-order release kinetics for neomycin during the first 20 h, followed by diffusion-controlled (Fickian) release for the remaining duration of the study. The release of neomycin suggested that the ability to load neomycin on to PLLA mats increases threefold, as the MW of the applied PEG coating is lowered from 20 kDa to 400 Da. Overall, this study demonstrates a successful approach to using a 3D printer to prepare porous degradable mats for antibiotic delivery with potential applications to dermal regeneration and tissue engineering.

scholarly journals 3D Printed Silicone Meniscus Implants: Influence of the 3D Printing Process on Properties of Silicone Implants

Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2136
Author(s):  
Eric Luis ◽  
Houwen Matthew Pan ◽  
Anil Kumar Bastola ◽  
Ram Bajpai ◽  
Swee Leong Sing ◽  
...  
Keyword(s):  
Photoelectron Spectroscopy ◽  
Weight Bearing ◽  
Thermo Gravimetric Analysis ◽  
Silicone Implants ◽  
Osteoarthritis Of The Knee ◽  
Gravimetric Analysis ◽  
Scanning Calorimetry ◽  
Hydrogel Scaffolds ◽  
Custom Made ◽  
3D Printed

Osteoarthritis of the knee with meniscal pathologies is a severe meniscal pathology suffered by the aging population worldwide. However, conventional meniscal substitutes are not 3D-printable and lack the customizability of 3D printed implants and are not mechanically robust enough for human implantation. Similarly, 3D printed hydrogel scaffolds suffer from drawbacks of being mechanically weak and as a result patients are unable to execute immediate post-surgical weight-bearing ambulation and rehabilitation. To solve this problem, we have developed a 3D silicone meniscus implant which is (1) cytocompatible, (2) resistant to cyclic loading and mechanically similar to native meniscus, and (3) directly 3D printable. The main focus of this study is to determine whether the purity, composition, structure, dimensions and mechanical properties of silicone implants are affected by the use of a custom-made in-house 3D-printer. We have used the phosphate buffer saline (PBS) absorption test, Fourier transform infrared (FTIR) spectroscopy, surface profilometry, thermo-gravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS), differential scanning calorimetry (DSC), and scanning electron microscopy (SEM) to effectively assess and compare material properties between molded and 3D printed silicone samples.

HBC-nanofiber hydrogel scaffolds with 3D printed internal microchannels for enhanced cartilage differentiation

2020 ◽  
Vol 8 (28) ◽  
pp. 6115-6127
Author(s):  
Xiaoyun Liu ◽  
Shaoshuai Song ◽  
Jie Huang ◽  
Han Fu ◽  
Xinyu Ning ◽  
...  
Keyword(s):  
Hydrogel Scaffolds ◽  
Cartilage Differentiation ◽  
3D Printed

HBC-nanofiber hydrogel scaffolds with 3D printed internal microchannels have been developed to provide a multifunctional biomimetic microenvironment for hMSC chondrogenesis.

scholarly journals Printability of 3D Printed Hydrogel Scaffolds: Influence of Hydrogel Composition and Printing Parameters

2019 ◽  
Vol 10 (1) ◽  
pp. 292 ◽  
Author(s):  
Saman Naghieh ◽  
MD Sarker ◽  
N. K. Sharma ◽  
Zohra Barhoumi ◽  
Xiongbiao Chen
Keyword(s):  
Flow Behavior ◽  
Three Dimensional ◽  
Methyl Cellulose ◽  
Alginate Hydrogel ◽  
Hydrogel Scaffolds ◽  
Number Of Factors ◽  
3D Printed ◽  
The Difference ◽  
Printing Processes ◽  
Printing Parameters

Extrusion-based bioprinting of hydrogel scaffolds is challenging due to printing-related issues, such as the lack of capability to precisely print or deposit hydrogels onto three-dimensional (3D) scaffolds as designed. Printability is an index to measure the difference between the designed and fabricated scaffold in the printing process, which, however, is still under-explored. While studies have been reported on printing hydrogel scaffolds from one or more hydrogels, there is limited knowledge on the printability of hydrogels and their printing processes. This paper presented our study on the printability of 3D printed hydrogel scaffolds, with a focus on identifying the influence of hydrogel composition and printing parameters/conditions on printability. Using the hydrogels synthesized from pure alginate or alginate with gelatin and methyl-cellulose, we examined their flow behavior and mechanical properties, as well as their influence on printability. To characterize the printability, we examined the pore size, strand diameter, and other dimensions of the printed scaffolds. We then evaluated the printability in terms of pore/strand/angular/printability and irregularity. Our results revealed that the printability could be affected by a number of factors and among them, the most important were those related to the hydrogel composition and printing parameters. This study also presented a framework to evaluate alginate hydrogel printability in a systematic manner, which can be adopted and used in the studies of other hydrogels for bioprinting.

scholarly journals 2153

2017 ◽  
Vol 1 (S1) ◽  
pp. 2-2
Author(s):  
Rahima Benhabbour ◽  
Rima Janusziewicz ◽  
Sue J. Mecham ◽  
Roopali Shrivastava ◽  
Gayane Paravyan
Keyword(s):  
Release Kinetics ◽  
Cost Effective ◽  
Unintended Pregnancies ◽  
Hiv Positive Mothers ◽  
Study Population ◽  
3D Printed ◽  
Injection Molded ◽  
In Vivo Characterization

OBJECTIVES/SPECIFIC AIMS: The long-term goal of this project is to develop a cost effective 3D printed multipurpose intravaginal ring (IVR) to prevent against unintended pregnancies and infectious diseases.Our goal is to develop a female-controlled method for prevention using innovative IVRs. METHODS/STUDY POPULATION: In vitro and in vivo characterization. RESULTS/ANTICIPATED RESULTS: Controlled and fine-tuned release kinetics 100% drug release from 3D printed IVRs compared with 10%–15% with traditional injection molded IVRs cost-effective engineering of multipurpose IVR with CLIP 3D printing technology. DISCUSSION/SIGNIFICANCE OF IMPACT: If successful, this project will revolutionize the engineering of IVRs and will have a global impact on human health. Not only we will help save millions of women around the world but also millions of children that are infected by their HIV-positive mothers through gestation or breast feeding.

scholarly journals 3D-Printed PCL Composite Scaffolds Incorporating IGF-1 Loaded PLGA Nanoparticles for Cartilage Tissue Engineering

2020 ◽  
Author(s):  
Peiran Wei ◽  
Yan Xu ◽  
Yue Gu ◽  
Qingqiang Yao ◽  
Jiayi Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Pore Size ◽  
Release Kinetics ◽  
Cartilage Tissue Engineering ◽  
Plga Nanoparticles ◽  
Contact Angles ◽  
Expression Level ◽  
Cartilage Tissue ◽  
Composite Scaffolds ◽  
3D Printed

Abstract Objective: To fabricate and test a 3D-printed PCL scaffold incorporating IGF-1 loaded PLGA nanoparticles for cartilage tissue engineering.Methods: IGF-1 loaded PLGA nanoparticles were produced by the double-emulsion method, and were incorporated onto 3D printed PCL scaffolds via PDA. Particle size, loading effciency (LE) and encapsulation effciency (EE) of the nanoparticles were examined. SEM, pore size, porosity, compression testing, contact angle, IGF-1 release kinetics of the composite scaffolds were also determined. For cell culture studies, CCK-8, Live/dead, MTT, GAG content and expression level of chondrocytes specific genes and HIF-1α were also tested.Results: There was no difference of the nanoparticle size. And the LE and EE of IGF-1 in PLGA nanoparticles was about 5.53%±0.12% and 61.26%±2.71%, respectively. There was a slower, sustained release for all drug-loaded nanoparticles PLGA/PDA/PCL scaffolds. There was no difference of pore size, porosity, compressive strength of each scaffold. The contact angles PCL scaffolds were significant decreased when coated with PDA and PLGA nanoparticales. (P < 0.05) Live/dead staining showed more cells attached to the IGF-1 PLGA/PDA/PCL scaffolds. The CCK-8 and MTT assay showed higher cell proliferation and better biocompatibility of the IGF-1 PLGA/PDA/PCL scaffolds. (P < 0.05) GAG content, chondrogenic gene expression level of SOX-9, COL-II, N-cadh, ACAN, and HIF pathway related gene(HIF-1α) were significantly higher in IGF-1 PLGA/PDA/PCL scaffolds on days 7 and 14 compared to other groups. (P < 0.05)Conclusions: IGF-1 PLGA/PDA/PCL scaffolds may be a better method for sustained IGF-1 administration and a promising scaffold for cartilage tissue engineering.

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