Elucidation of the Dexter-Type Energy Transfer in DNA by Thymine-Thymine Dimer Formation Using Photosensitizers as Artificial Nucleosides

2016 ◽  
Vol 56 (5) ◽  
pp. 1385-1389 ◽  
Author(s):  
Linda Antusch ◽  
Nadine Gaß ◽  
Hans-Achim Wagenknecht
Keyword(s):  
Energy Transfer ◽  
Dimer Formation ◽  
Thymine Dimer

scholarly journals Flavonols Protect Against UV Radiation-Induced Thymine Dimer Formation in an Artificial Skin Mimic

2015 ◽  
Vol 18 (4) ◽  
pp. 600 ◽  
Author(s):  
Sabia Maini ◽  
Brian M. Fahlman ◽  
Ed S. Krol
Keyword(s):  
Uv Radiation ◽  
Artificial Skin ◽  
Uvb Radiation ◽  
Skin Damage ◽  
Dimer Formation ◽  
Thymine Dimer ◽  
Matrix Metalloproteinase 1 ◽  
Pyrimidine Dimers ◽  
Limit Of Quantitation ◽  
Radiation Induced

Purpose: Exposure of skin to ultraviolet light has been shown to have a number of deleterious effects including photoaging, photoimmunosuppression and photoinduced DNA damage which can lead to the development of skin cancer.  In this paper we present a study on the ability of three flavonols to protect EpiDerm™, an artificial skin mimic, against UV-induced damage. Methods: EpiDerm™ samples were treated with flavonol in acetone and exposed to UVA (100 kJ/m2 at 365 nm) and UVB (9000 J/m2 at 310 nm) radiation.  Secretion of matrix metalloproteinase-1 (MMP-1) and tumor necrosis factor-α (TNF-a) were determined by ELISA, cyclobutane pyrimidine dimers were quantified using LC-APCI-MS. Results: EpiDerm ™ treated topically with quercetin significantly decreased MMP-1 secretion induced by UVA (100 µM) or UVB (200 µM) and TNF-a secretion was significantly reduced at 100 µM quercetin for both UVA and UVB radiation.  In addition, topically applied quercetin was found to be photostable over the duration of the experiment.  EpiDerm™ samples were treated topically with quercetin, kaempferol or galangin (52 µM) immediately prior to UVA or UVB exposure, and the cyclobutane thymine dimers (T-T (CPD)) were quantified using an HPLC-APCI MS/MS method.  All three flavonols significantly decreased T-T (CPD) formation in UVB irradiated EpiDerm™, however no effect could be observed for the UVA irradiation experiments as thymine dimer formation was below the limit of quantitation.  Conclusions: Our results suggest that flavonols can provide protection against UV radiation-induced skin damage through both antioxidant activity and direct photo-absorption. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

scholarly journals Time-Resolved Study of Thymine Dimer Formation

2005 ◽  
Vol 127 (16) ◽  
pp. 5780-5781 ◽  
Author(s):  
Sylvie Marguet ◽  
Dimitra Markovitsi
Keyword(s):  
Dimer Formation ◽  
Thymine Dimer ◽  
Time Resolved

Thymine Dimer Formation probed by Time-Resolved Vibrational Spectroscopy

2007 ◽  
pp. 522-524
Author(s):  
Wolfgang J. Schreier ◽  
Tobias E. Schrader ◽  
Florian O. Roller ◽  
Peter Gilch ◽  
Wolfgang Zinth ◽  
...  
Keyword(s):  
Vibrational Spectroscopy ◽  
Dimer Formation ◽  
Thymine Dimer ◽  
Time Resolved
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