Dirigent Proteins from Cotton (Gossypiumsp.) for the Atropselective Synthesis of Gossypol

2015 ◽  
Vol 54 (49) ◽  
pp. 14660-14663 ◽  
Author(s):  
Isabelle Effenberger ◽  
Bin Zhang ◽  
Ling Li ◽  
Qiang Wang ◽  
Yuxiu Liu ◽  
...  
Keyword(s):  
Dirigent Proteins

Expression in Pichia pastoris and characterization of two novel dirigent proteins for atropselective formation of gossypol

2016 ◽  
Vol 101 (5) ◽  
pp. 2021-2032 ◽  
Author(s):  
Isabelle Effenberger ◽  
Michael Harport ◽  
Jens Pfannstiel ◽  
Iris Klaiber ◽  
Andreas Schaller
Keyword(s):  
Pichia Pastoris ◽  
Dirigent Proteins

scholarly journals A model of dirigent proteins derived from structural and functional similarities with allene oxide cyclase and lipocalins

FEBS Journal ◽  
2012 ◽  
Vol 279 (11) ◽  
pp. 1980-1993 ◽  
Author(s):  
Benjamin Pickel ◽  
Jens Pfannstiel ◽  
Alexander Steudle ◽  
Axel Lehmann ◽  
Uwe Gerken ◽  
...  
Keyword(s):  
Allene Oxide ◽  
Allene Oxide Cyclase ◽  
Dirigent Proteins

scholarly journals Opposite Stereoselectivities of Dirigent Proteins inArabidopsisandSchizandraSpecies

2012 ◽  
Vol 287 (41) ◽  
pp. 33957-33972 ◽  
Author(s):  
Kye-Won Kim ◽  
Syed G. A. Moinuddin ◽  
Kathleen M. Atwell ◽  
Michael A. Costa ◽  
Laurence B. Davin ◽  
...  
Keyword(s):  
Dirigent Proteins

scholarly journals A soybean NAC homolog contributes to resistance to Phytophthora sojae mediated by dirigent proteins

2021 ◽  
Author(s):  
Guolong Yu ◽  
Jianan Zou ◽  
Jinhui Wang ◽  
Rongsheng Zhu ◽  
Zhaoming Qi ◽  
...  
Keyword(s):  
Phytophthora Sojae ◽  
Dirigent Proteins

scholarly journals De novo Sequencing and Native Mass Spectrometry Reveals Hetero-Association of Dirigent Protein Homologs and Potential Interacting Proteins in Forsythia × intermedia

The Analyst ◽  
2021 ◽  
Author(s):  
Mowei Zhou ◽  
Joseph A Laureanti ◽  
Callum Bell ◽  
Mi Kwon ◽  
Qingyan Meng ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
De Novo ◽  
Native Mass Spectrometry ◽  
De Novo Sequencing ◽  
Interacting Proteins ◽  
Dirigent Protein ◽  
Radical Coupling ◽  
Dirigent Proteins

The discovery of dirigent proteins (DPs) and their functions in plant phenol biochemistry was made over two decades ago with Forsythia × intermedia. Stereo-selective, DP-guided, monolignol-derived radical coupling in vitro...

scholarly journals Pterocarpan synthase (PTS) structures suggest a common quinone methide–stabilizing function in dirigent proteins and proteins with dirigent-like domains

2020 ◽  
Vol 295 (33) ◽  
pp. 11584-11601 ◽  
Author(s):  
Qingyan Meng ◽  
Syed G. A. Moinuddin ◽  
Sung-Jin Kim ◽  
Diana L. Bedgar ◽  
Michael A. Costa ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Land Plant ◽  
Plant Evolution ◽  
Quinone Methide ◽  
3D Structures ◽  
Bond Forming ◽  
Biochemical Mechanisms ◽  
Land Plant Evolution ◽  
Dirigent Proteins ◽  
Diverse Plant

The biochemical activities of dirigent proteins (DPs) give rise to distinct complex classes of plant phenolics. DPs apparently began to emerge during the aquatic-to-land transition, with phylogenetic analyses revealing the presence of numerous DP subfamilies in the plant kingdom. The vast majority (>95%) of DPs in these large multigene families still await discovery of their biochemical functions. Here, we elucidated the 3D structures of two pterocarpan-forming proteins with dirigent-like domains. Both proteins stereospecifically convert distinct diastereomeric chiral isoflavonoid precursors to the chiral pterocarpans, (–)- and (+)-medicarpin, respectively. Their 3D structures enabled comparisons with stereoselective lignan– and aromatic terpenoid–forming DP orthologs. Each protein provides entry into diverse plant natural products classes, and our experiments suggest a common biochemical mechanism in binding and stabilizing distinct plant phenol–derived mono- and bis-quinone methide intermediates during different C–C and C–O bond–forming processes. These observations provide key insights into both their appearance and functional diversification of DPs during land plant evolution/adaptation. The proposed biochemical mechanisms based on our findings provide important clues to how additional physiological roles for DPs and proteins harboring dirigent-like domains can now be rationally and systematically identified.

Dirigent proteins and dirigent sites in lignifying tissues

2001 ◽  
Vol 57 (6) ◽  
pp. 883-897 ◽  
Author(s):  
Vincent Burlat ◽  
Mi Kwon ◽  
Laurence B. Davin ◽  
Norman G. Lewis
Keyword(s):  
Dirigent Proteins
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