Visualization of Oxygen Consumption of Single Living Cells by Scanning Electrochemical Microscopy: The Influence of the Faradaic Tip Reaction

2013 ◽  
Vol 52 (24) ◽  
pp. 6335-6338 ◽  
Author(s):  
Michaela Nebel ◽  
Stefanie Grützke ◽  
Nizam Diab ◽  
Albert Schulte ◽  
Wolfgang Schuhmann
Keyword(s):  
Oxygen Consumption ◽  
Scanning Electrochemical Microscopy ◽  
Living Cells ◽  
Single Living Cells ◽  
Single Living ◽  
Electrochemical Microscopy

Microelectrochemical visualization of oxygen consumption of single living cells

2013 ◽  
Vol 164 ◽  
pp. 19 ◽  
Author(s):  
Michaela Nebel ◽  
Stefanie Grützke ◽  
Nizam Diab ◽  
Albert Schulte ◽  
Wolfgang Schuhmann
Keyword(s):  
Oxygen Consumption ◽  
Living Cells ◽  
Single Living Cells ◽  
Single Living

Application of FRAP and digitized fluorescence microscopy to problems in cell membrane dynamics

1988 ◽  
Vol 46 ◽  
pp. 118-119
Author(s):  
K. Jacobson ◽  
A. Ishihara ◽  
B. Holifield ◽  
F. Zhang
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Biology ◽  
Extended Period ◽  
Dynamic Structure ◽  
Living Cells ◽  
Membrane Dynamics ◽  
Molecular Cell Biology ◽  
Image Averaging ◽  
Single Living Cells ◽  
Single Living

Our laboratory is concerned with understanding the dynamic structure of the plasma membrane with particular reference to the movement of membrane constituents during cell locomotion. In addition to the standard tools of molecular cell biology, we employ both fluorescence recovery after photo- bleaching (FRAP) and digitized fluorescence microscopy (DFM) to investigate individual cells. FRAP allows the measurement of translational mobility of membrane and cytoplasmic molecules in small regions of single, living cells. DFM is really a new form of light microscopy in that the distribution of individual classes of ions, molecules, and macromolecules can be followed in single, living cells. By employing fluorescent antibodies to defined antigens or fluorescent analogs of cellular constituents as well as ultrasensitive, electronic image detectors and video image averaging to improve signal to noise, fluorescent images of living cells can be acquired over an extended period without significant fading and loss of cell viability.

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