scholarly journals Discovery of Catalytic Peptides for Inorganic Nanocrystal Synthesis by a Combinatorial Phage Display Approach

2011 ◽  
Vol 50 (45) ◽  
pp. 10585-10588 ◽  
Author(s):  
Zengyan Wei ◽  
Yoshiaki Maeda ◽  
Hiroshi Matsui
Keyword(s):  
Phage Display ◽  
Nanocrystal Synthesis ◽  
Catalytic Peptides

Discovery of Catalytic Peptides for Inorganic Nanocrystal Synthesis by a Combinatorial Phage Display Approach

2011 ◽  
Vol 123 (45) ◽  
pp. 10773-10776 ◽  
Author(s):  
Zengyan Wei ◽  
Yoshiaki Maeda ◽  
Hiroshi Matsui
Keyword(s):  
Phage Display ◽  
Nanocrystal Synthesis ◽  
Catalytic Peptides

Combination of ribosome and phage display for fast selection of high affinity VHH antibody fragments

2019 ◽  
pp. 27-33
Author(s):  
YuE Kravchenko ◽  
SV Ivanov ◽  
DS Kravchenko ◽  
EI Frolova ◽  
SP Chumakov
Keyword(s):  
Phage Display ◽  
Selection Procedure ◽  
Free System ◽  
Phage Displays ◽  
High Affinity ◽  
Binding Domains ◽  
A Cell ◽  
Vhh Antibodies ◽  
Efficiency Of Selection ◽  
Selection Of

Selection of antibodies using phage display involves the preliminary cloning of the repertoire of sequences encoding antigen-binding domains into phagemid, which is considered the bottleneck of the method, limiting the resulting diversity of libraries and leading to the loss of poorly represented variants before the start of the selection procedure. Selection in cell-free conditions using a ribosomal display is devoid from this drawback, however is highly sensitive to PCR artifacts and the RNase contamination. The aim of the study was to test the efficiency of a combination of both methods, including pre-selection in a cell-free system to enrich the source library, followed by cloning and final selection using phage display. This approach may eliminate the shortcomings of each method and increase the efficiency of selection. For selection, alpaca VHH antibody sequences suitable for building an immune library were used due to the lack of VL domains. Analysis of immune libraries from the genes of the VH3, VHH3 and VH4 families showed that the VHH antibodies share in the VH3 and VH4 gene groups is insignificant, and selection from the combined library is less effective than from the VHH3 family of sequences. We found that the combination of ribosomal and phage displays leads to a higher enrichment of high-affinity fragments and avoids the loss of the original diversity during cloning. The combined method allowed us to obtain a greater number of different high-affinity sequences, and all the tested VHH fragments were able to specifically recognize the target, including the total protein extracts of cell cultures.

Solvent-Ligand Interactions in Colloidal Nanocrystals

2018 ◽  
Author(s):  
Jonathan De Roo ◽  
Nuri Yazdani ◽  
Emile Drijvers ◽  
Alessandro Lauria ◽  
Jorick Maes ◽  
...  
Keyword(s):  
Direct Method ◽  
Line Broadening ◽  
H Nmr ◽  
Size Dependent ◽  
Line Shape Analysis ◽  
Wide Range ◽  
Nanocrystal Synthesis ◽  
Ligand Interactions ◽  
Indispensable Tool ◽  
Theoretical Evidence

<p>Although solvent-ligand interactions play a major role in nanocrystal synthesis, dispersion formulation and assembly, there is currently no direct method to study this. Here we examine the broadening of <sup>1</sup>H NMR resonances associated with bound ligands, and turn this poorly understood descriptor into a tool to assess solvent-ligand interactions. We show that the line broadening has both a homogeneous and a heterogeneous component. The former is nanocrystal-size dependent and the latter results from solvent-ligand interactions. Our model is supported by experimental and theoretical evidence that correlates broad NMR lines with poor ligand solvation. This correlation is found across a wide range of solvents, extending from water to hexane, for both hydrophobic and hydrophilic ligand types, and for a multitude of oxide, sulfide and selenide nanocrystals. Our findings thus put forward NMR line shape analysis as an indispensable tool to form, investigate and manipulate nanocolloids.</p>

Protein Chemical Synthesis Combined with Mirror-Image Phage Display Yields D-Peptide EGF Ligands that Block the EGF-EGFR Interaction

2019 ◽  
Author(s):  
Cristina D&iacute;az-Perlas ◽  
Monica Varese ◽  
Salvador Guardiola ◽  
Macarena S&aacute;nchez-Navarro ◽  
Jesús García ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
Phage Display ◽  
Mirror Image
Sign in / Sign up

Export Citation Format

Share Document