Site-Specific Synthesis and In Situ Immobilization of Fluorescent Silver Nanoclusters on DNA Nanoscaffolds by Use of the Tollens Reaction

2011 ◽  
Vol 50 (18) ◽  
pp. 4176-4179 ◽  
Author(s):  
Suchetan Pal ◽  
Reji Varghese ◽  
Zhengtao Deng ◽  
Zhao Zhao ◽  
Ashok Kumar ◽  
...  
Keyword(s):  
Silver Nanoclusters ◽  
In Situ Immobilization ◽  
Site Specific ◽  
Specific Synthesis

Site-Specific Synthesis and In Situ Immobilization of Fluorescent Silver Nanoclusters on DNA Nanoscaffolds by Use of the Tollens Reaction

2011 ◽  
Vol 123 (18) ◽  
pp. 4262-4265 ◽  
Author(s):  
Suchetan Pal ◽  
Reji Varghese ◽  
Zhengtao Deng ◽  
Zhao Zhao ◽  
Ashok Kumar ◽  
...  
Keyword(s):  
Silver Nanoclusters ◽  
In Situ Immobilization ◽  
Site Specific ◽  
Specific Synthesis

Anthracyclines. XI. A short, site-specific synthesis of unsymmetrical 3-Acetyl-5,8-dialkoxy-1,2-dihydronaphthalenes; key precursors to daunomycinone AB-synthons

1984 ◽  
Vol 37 (8) ◽  
pp. 1721 ◽  
Author(s):  
RA Russell ◽  
GJ Collin ◽  
MP Crane ◽  
PS Gee ◽  
AS Krauss ◽  
...  
Keyword(s):  
Acetyl Chloride ◽  
Cost Effective ◽  
Base Hydrolysis ◽  
Chromatographic Separations ◽  
Phenol Alkylation ◽  
Site Specific ◽  
Effective Synthesis ◽  
Hydrolysis Of ◽  
Specific Synthesis

5,8-Dihydronaphthalene-1,4-diol, readily available from benzoquinone and buta-1,3-diene, is isomerized by heating with strong sodium hydroxide, and acetylated (Ac2O), in situ, to form 5,8-diacetoxy-1,2-dihydronaphthalene. Catalysed (AlCl3) addition of acetyl chloride followed by dehydrochlorination (LiCI/HCONMe2) yielded 5,8-diacetoxy-3-acetyl-1,2-dihydronaphthalene (11) in 82% overall yield. Base hydrolysis of (11), followed by methylation (Me2SO4) gives 3-acetyl-5,8-dimethoxy-1,2-dihydronaphthalene in 94% yield, the most direct route to this product so far described. More importantly, the diacetate (11) is selectively deacetylated at C5 (Cs2CO3 in tetrahydrofuran, or K2CO3 in Me2SO) to form the related phenol, alkylation of which produces the 5-alkoxy compound. Further hydrolysis followed by alkylation yields the unsymmetrically, but regiospecifically substituted, 3-acetyl-5,8-dialkoxy-1,2-dihydronaphthalenes. This method is specifically illustrated by the production of 3-acetyl-5-benzyloxy-8-methoxy-1,2-dihydronaphthalene which is formed in a short, cost effective synthesis in a moderate overall yield (25%) based upon benzoquinone as starting material;significantly, no chromatographic separations are required.

To Eliminate Curtain Effect of FIB TEM Samples by a Combination of Sample Dicing and Backside Milling

2014 ◽  
Author(s):  
Jian-Shing Luo ◽  
Hsiu Ting Lee
Keyword(s):  
Sample Preparation ◽  
Focused Ion Beam ◽  
Preparation Method ◽  
Low Cost ◽  
Ion Beam ◽  
Sample Preparation Method ◽  
Site Specific ◽  
Dual Beam ◽  
Transmission Electron

Abstract Several methods are used to invert samples 180 deg in a dual beam focused ion beam (FIB) system for backside milling by a specific in-situ lift out system or stages. However, most of those methods occupied too much time on FIB systems or requires a specific in-situ lift out system. This paper provides a novel transmission electron microscopy (TEM) sample preparation method to eliminate the curtain effect completely by a combination of backside milling and sample dicing with low cost and less FIB time. The procedures of the TEM pre-thinned sample preparation method using a combination of sample dicing and backside milling are described step by step. From the analysis results, the method has applied successfully to eliminate the curtain effect of dual beam FIB TEM samples for both random and site specific addresses.

scholarly journals Cell-free protein synthesis and in situ immobilization of deGFP-MatB in polymer microgels for malonate-to-malonyl CoA conversion

RSC Advances ◽  
2020 ◽  
Vol 10 (66) ◽  
pp. 40588-40596
Author(s):  
Tony Köhler ◽  
Thomas Heida ◽  
Sandra Hoefgen ◽  
Niclas Weigel ◽  
Vito Valiante ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Genetic Information ◽  
In Situ Immobilization ◽  
Bottom Up ◽  
Free Protein ◽  
Malonyl Coa ◽  
Cell Free Protein Synthesis

We describe a bottom-up approach towards functional enzymes utilizing microgels as carriers for genetic information that enable cell-free protein synthesis, in situ immobilization, and utilization of functional deGFP-MatB.

In situ immobilization of Candida antarctica B lipase in polyurethane foam support

2016 ◽  
Vol 124 ◽  
pp. 52-61 ◽  
Author(s):  
Nadia Ligianara D. Nyari ◽  
Ilizandra A. Fernandes ◽  
Cindy E. Bustamante-Vargas ◽  
Clarisse Steffens ◽  
Débora de Oliveira ◽  
...  
Keyword(s):  
Polyurethane Foam ◽  
Candida Antarctica ◽  
In Situ Immobilization ◽  
Candida Antarctica B ◽  
Candida Antarctica B Lipase

Molecular modulation of calcium oxalate crystallization

2006 ◽  
Vol 291 (6) ◽  
pp. F1123-F1132 ◽  
Author(s):  
James J. De Yoreo ◽  
S. Roger Qiu ◽  
John R. Hoyer
Keyword(s):  
Molecular Modeling ◽  
Calcium Oxalate ◽  
Stone Formation ◽  
Energy Levels ◽  
Critical Role ◽  
Specific Interactions ◽  
Crystal Surfaces ◽  
Site Specific

Calcium oxalate monohydrate (COM) is the primary constituent of the majority of renal stones. Osteopontin (OPN), an aspartic acid-rich urinary protein, and citrate, a much smaller molecule, are potent inhibitors of COM crystallization at levels present in normal urine. Current concepts of the role of site-specific interactions in crystallization derived from studies of biomineralization are reviewed to provide a context for understanding modulation of COM growth at a molecular level. Results from in situ atomic force microscopy (AFM) analyses of the effects of citrate and OPN on growth verified the critical role of site-specific interactions between these growth modulators and individual steps on COM crystal surfaces. Molecular modeling investigations of interactions of citrate with steps and faces on COM crystal surfaces provided links between the stereochemistry of interaction and the binding energy levels that underlie mechanisms of growth modification and changes in overall crystal morphology. The combination of in situ AFM and molecular modeling provides new knowledge that will aid rationale design of therapeutic agents for inhibition of stone formation.

Covalent binding and in-situ immobilization of lipases on a flexible nanoporous material

2021 ◽  
Vol 102 ◽  
pp. 92-101
Author(s):  
Saba Ghasemi ◽  
Maryam Yousefi ◽  
Ahmad Nikseresht ◽  
Hoda Omidi
Keyword(s):  
Covalent Binding ◽  
Nanoporous Material ◽  
In Situ Immobilization
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