Crystal Structure of a Cisplatin-(1,3-GTG) Cross-Link within DNA Polymerase η

2010 ◽  
Vol 49 (17) ◽  
pp. 3077-3080 ◽  
Author(s):  
Thomas Reißner ◽  
Sabine Schneider ◽  
Stephanie Schorr ◽  
Thomas Carell
Keyword(s):  
Crystal Structure ◽  
Dna Polymerase ◽  
Cross Link

Cover Picture: Crystal Structure of a Cisplatin-(1,3-GTG) Cross-Link within DNA Polymerase η (Angew. Chem. Int. Ed. 17/2010)

2010 ◽  
Vol 49 (17) ◽  
pp. 2949-2949 ◽  
Author(s):  
Thomas Reißner ◽  
Sabine Schneider ◽  
Stephanie Schorr ◽  
Thomas Carell
Keyword(s):  
Crystal Structure ◽  
Dna Polymerase ◽  
Cross Link

Model of the Second Most Abundant Cisplatin−DNA Cross-Link:  X-ray Crystal Structure and Conformational Analysis ofcis-[(NH3)2Pt(9-MeA-N7)(9-EtGH-N7)](NO3)·2H2O (9-MeA = 9-Methyladenine; 9-EtGH = 9-Ethylguanine)

1996 ◽  
Vol 35 (6) ◽  
pp. 1647-1652 ◽  
Author(s):  
Guy Schröder ◽  
Jirí Kozelka ◽  
Michal Sabat ◽  
Marie-Hélène Fouchet ◽  
Rut Beyerle-Pfnür ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Conformational Analysis ◽  
Cross Link ◽  
X Ray

scholarly journals Replication Past the -Radiation-Induced Guanine-Thymine Cross-Link G[8,5-Me]T by Human and Yeast DNA Polymerase

2010 ◽  
Vol 2010 ◽  
pp. 1-10 ◽  
Author(s):  
Paromita Raychaudhury ◽  
Ashis K. Basu
Keyword(s):  
Dna Polymerase ◽  
Mammalian Cells ◽  
Translesion Synthesis ◽  
Kidney Cells ◽  
Equal Frequency ◽  
Cross Link ◽  
Dominant Mutation ◽  
Human Embryonic Kidney Cells ◽  
Radiation Induced

-Radiation-induced intrastrand guanine-thymine cross-link, G[8,5-Me]T, hinders replicationin vitroand is mutagenic in mammalian cells. Herein we reportin vitrotranslesion synthesis of G[8,5-Me]T by human and yeast DNA polymerase (hPol and yPol ). dAMP misincorporation opposite the cross-linked G by yPol was preferred over correct incorporation of dCMP, but further extension was 100-fold less efficient for :A compared to :C. For hPol , both incorporation and extension were more efficient with the correct nucleotides. To evaluate translesion synthesis in the presence of all four dNTPs, we have developed a plasmid-based DNA sequencing assay, which showed that yPol was more error-prone. Mutational frequencies of yPol and hPol were 36% and 14%, respectively. Targeted was the dominant mutation by both DNA polymerases. But yPol induced targeted in 23% frequency relative to 4% by hPol . For yPol , targeted and constituted 83% of the mutations. By contrast, with hPol , semi-targeted mutations (7.2%), that is, mutations at bases near the lesion, occurred at equal frequency as the targeted mutations (6.9%). The kind of mutations detected with hPol showed significant similarities with the mutational spectrum of G[8,5-Me]T in human embryonic kidney cells.

scholarly journals Crystal Structure of a pol α Family Replication DNA Polymerase from Bacteriophage RB69

Cell ◽  
1997 ◽  
Vol 89 (7) ◽  
pp. 1087-1099 ◽  
Author(s):  
J. Wang ◽  
A.K.M.A. Sattar ◽  
C.C. Wang ◽  
J.D. Karam ◽  
W.H. Konigsberg ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Dna Polymerase ◽  
Bacteriophage Rb69

scholarly journals Crystal structure of DNA polymerase from bacteriophage M2

2005 ◽  
Vol 61 (a1) ◽  
pp. c221-c221
Author(s):  
H. Hashimoto ◽  
T. Shimizu ◽  
M. Sato ◽  
H. Koda ◽  
Y. Hashimoto ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Dna Polymerase

scholarly journals Crystal Structure of the Plasmodium falciparum Apicoplast DNA Polymerase

2015 ◽  
Vol 29 (S1) ◽  
Author(s):  
Morgan Milton ◽  
Richard Honzatko ◽  
Scott Nelson
Keyword(s):  
Crystal Structure ◽  
Plasmodium Falciparum ◽  
Dna Polymerase

scholarly journals The Crystal Structure of Cytochrome P460 ofNitrosomonas europaeaReveals a Novel Cytochrome Fold and Heme−Protein Cross-link†,‡

Biochemistry ◽  
2007 ◽  
Vol 46 (28) ◽  
pp. 8340-8349 ◽  
Author(s):  
Arwen R. Pearson ◽  
Bradley O. Elmore ◽  
Cheng Yang ◽  
Joseph D. Ferrara ◽  
Alan B. Hooper ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Heme Protein ◽  
Cross Link

scholarly journals Structural analysis of point mutations at theVaccinia virusA20/D4 interface

2016 ◽  
Vol 72 (9) ◽  
pp. 687-691 ◽  
Author(s):  
Céline Contesto-Richefeu ◽  
Nicolas Tarbouriech ◽  
Xavier Brazzolotto ◽  
Wim P. Burmeister ◽  
Christophe N. Peyrefitte ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Amino Acids ◽  
Structural Analysis ◽  
Complex Formation ◽  
Dna Polymerase ◽  
Point Mutations ◽  
Conformational Space ◽  
Uracil Dna Glycosylase ◽  
Dimerization Interface ◽  
Solvation Parameters

TheVaccinia viruspolymerase holoenzyme is composed of three subunits: E9, the catalytic DNA polymerase subunit; D4, a uracil-DNA glycosylase; and A20, a protein with no known enzymatic activity. The D4/A20 heterodimer is the DNA polymerase cofactor, the function of which is essential for processive DNA synthesis. The recent crystal structure of D4 bound to the first 50 amino acids of A20 (D4/A201–50) revealed the importance of three residues, forming a cation–π interaction at the dimerization interface, for complex formation. These are Arg167 and Pro173 of D4 and Trp43 of A20. Here, the crystal structures of the three mutants D4-R167A/A201–50, D4-P173G/A201–50and D4/A201–50-W43A are presented. The D4/A20 interface of the three structures has been analysed for atomic solvation parameters and cation–π interactions. This study confirms previous biochemical data and also points out the importance for stability of the restrained conformational space of Pro173. Moreover, these new structures will be useful for the design and rational improvement of known molecules targeting the D4/A20 interface.

The crystal structure of a natural DNA polymerase complexed with mirror DNA

2020 ◽  
Vol 56 (14) ◽  
pp. 2186-2189
Author(s):  
Jinsu An ◽  
Jaewoo Choi ◽  
Dohyeon Hwang ◽  
Jihyun Park ◽  
Charles W. Pemble ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Dna Binding ◽  
Dna Polymerase ◽  
Binding Properties ◽  
Dna Binding Properties

The intrinsic l-DNA binding properties of a natural DNA polymerase was discovered.

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