Inside Cover: Exploring the Conformation-Regulated Function of Titin Kinase by Mechanical Pump and Probe Experiments with Single Molecules (Angew. Chem. Int. Ed. 6/2010)

Elias M. Puchner; Hermann E. Gaub

doi:10.1002/anie.200907116

Inside Cover: Exploring the Conformation-Regulated Function of Titin Kinase by Mechanical Pump and Probe Experiments with Single Molecules (Angew. Chem. Int. Ed. 6/2010)

Angewandte Chemie International Edition ◽

10.1002/anie.200907116 ◽

2010 ◽

Vol 49 (6) ◽

pp. 990-990

Author(s):

Elias M. Puchner ◽

Hermann E. Gaub

Keyword(s):

Single Molecules ◽

Pump And Probe ◽

Mechanical Pump ◽

Titin Kinase

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Exploring the Conformation-Regulated Function of Titin Kinase by Mechanical Pump and Probe Experiments with Single Molecules

Angewandte Chemie International Edition ◽

10.1002/anie.200905956 ◽

2010 ◽

Vol 49 (6) ◽

pp. 1147-1150 ◽

Cited By ~ 26

Author(s):

Elias M. Puchner ◽

Hermann E. Gaub

Keyword(s):

Single Molecules ◽

Pump And Probe ◽

Mechanical Pump ◽

Titin Kinase

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Exploring the Function of Titin Kinase by Mechanical Single-Molecule Pump-And-Probe

Biophysical Journal ◽

10.1016/j.bpj.2009.12.4124 ◽

2010 ◽

Vol 98 (3) ◽

pp. 752a

Author(s):

Elias M. Puchner ◽

Hermann E. Gaub

Keyword(s):

Single Molecule ◽

Pump And Probe ◽

Titin Kinase

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Studies on Water in the Hydration Chamber of a Modified Electron Microscope

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100069715 ◽

1970 ◽

Vol 28 ◽

pp. 544-545

Author(s):

R. C. Moretz ◽

G. G. Hausner ◽

D. F. Parsons

Keyword(s):

Thin Film ◽

Thin Films ◽

Electron Microscope ◽

Steady State ◽

Room Temperature ◽

Small Mass ◽

Equilibrium Pressure ◽

Biological Objects ◽

Mechanical Pump ◽

Differential Pumping

Use of the electron microscope to examine wet objects is possible due to the small mass thickness of the equilibrium pressure of water vapor at room temperature. Previous attempts to examine hydrated biological objects and water itself used a chamber consisting of two small apertures sealed by two thin films. Extensive work in our laboratory showed that such films have an 80% failure rate when wet. Using the principle of differential pumping of the microscope column, we can use open apertures in place of thin film windows.Fig. 1 shows the modified Siemens la specimen chamber with the connections to the water supply and the auxiliary pumping station. A mechanical pump is connected to the vapor supply via a 100μ aperture to maintain steady-state conditions.

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