A Virosome-Mimotope Approach to Synthetic Vaccine Design and Optimization: Synthesis, Conformation, and Immune Recognition of a Potential Malaria-Vaccine Candidate

2003 ◽  
Vol 42 (21) ◽  
pp. 2368-2371 ◽  
Author(s):  
Bernhard Pfeiffer ◽  
Elisabetta Peduzzi ◽  
Kerstin Moehle ◽  
Rinaldo Zurbriggen ◽  
Reinhard Glück ◽  
...  
Keyword(s):  
Malaria Vaccine ◽  
Vaccine Candidate ◽  
Vaccine Design ◽  
Immune Recognition ◽  
Design And Optimization ◽  
Malaria Vaccine Candidate

scholarly journals Guiding the Immune Response to a Conserved Epitope in MSP2, an Intrinsically Disordered Malaria Vaccine Candidate

Vaccines ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 855
Author(s):  
Jeffrey Seow ◽  
Sreedam C. Das ◽  
Rodrigo A. V. Morales ◽  
Ricardo Ataide ◽  
Bankala Krishnarjuna ◽  
...  
Keyword(s):  
Immune Response ◽  
Malaria Vaccine ◽  
Vaccine Candidate ◽  
Single Point ◽  
Peptide Vaccine ◽  
Vaccine Design ◽  
Single Point Mutation ◽  
Strain Specificity ◽  
Intrinsically Disordered ◽  
Malaria Vaccine Candidate

The malaria vaccine candidate merozoite surface protein 2 (MSP2) has shown promise in clinical trials and is in part responsible for a reduction in parasite densities. However, strain-specific reductions in parasitaemia suggested that polymorphic regions of MSP2 are immuno-dominant. One strategy to bypass the hurdle of strain-specificity is to bias the immune response towards the conserved regions. Two mouse monoclonal antibodies, 4D11 and 9H4, recognise the conserved C-terminal region of MSP2. Although they bind overlapping epitopes, 4D11 reacts more strongly with native MSP2, suggesting that its epitope is more accessible on the parasite surface. In this study, a structure-based vaccine design approach was applied to the intrinsically disordered antigen, MSP2, using a crystal structure of 4D11 Fv in complex with its minimal binding epitope. Molecular dynamics simulations and surface plasmon resonance informed the design of a series of constrained peptides that mimicked the 4D11-bound epitope structure. These peptides were conjugated to keyhole limpet hemocyanin and used to immunise mice, with high to moderate antibody titres being generated in all groups. The specificities of antibody responses revealed that a single point mutation can focus the antibody response towards a more favourable epitope. This structure-based approach to peptide vaccine design may be useful not only for MSP2-based malaria vaccines, but also for other intrinsically disordered antigens.

scholarly journals Optimizing expression of the pregnancy malaria vaccine candidate, VAR2CSA in Pichia pastoris

2009 ◽  
Vol 8 (1) ◽  
pp. 143 ◽  
Author(s):  
Marion Avril ◽  
Marianne J Hathaway ◽  
Megan M Cartwright ◽  
Severin O Gose ◽  
David L Narum ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Malaria Vaccine ◽  
Vaccine Candidate ◽  
Malaria Vaccine Candidate ◽  
Pregnancy Malaria

Liposome engraftment and antigen combination potentiate the immune response towards conserved epitopes of the malaria vaccine candidate MSP2

Vaccine ◽  
2021 ◽  
Author(s):  
Sreedam C. Das ◽  
Jason D. Price ◽  
Katharine Gosling ◽  
Nicola MacLennan ◽  
Ricardo Ataíde ◽  
...  
Keyword(s):  
Immune Response ◽  
Malaria Vaccine ◽  
Vaccine Candidate ◽  
Malaria Vaccine Candidate

scholarly journals A Plant-Produced Pfs25 VLP Malaria Vaccine Candidate Induces Persistent Transmission Blocking Antibodies against Plasmodium falciparum in Immunized Mice

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e79538 ◽  
Author(s):  
R. Mark Jones ◽  
Jessica A. Chichester ◽  
Vadim Mett ◽  
Jennifer Jaje ◽  
Stephen Tottey ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Malaria Vaccine ◽  
Vaccine Candidate ◽  
Transmission Blocking ◽  
Malaria Vaccine Candidate ◽  
Blocking Antibodies
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