scholarly journals Discovery of oligosaccharide antigens for semi‐synthetic glycoconjugate vaccine leads against Streptococcus suis serotypes 2, 3, 9 and 14

2021 ◽  
Author(s):  
Shuo Zhang ◽  
Mauro Sella ◽  
Julinton Sianturi ◽  
Patricia Priegue ◽  
Dacheng Shen ◽  
...  
Keyword(s):  
Streptococcus Suis ◽  
Glycoconjugate Vaccine

scholarly journals Protection against Streptococcus suis Serotype 2 Infection Using a Capsular Polysaccharide Glycoconjugate Vaccine

2016 ◽  
Vol 84 (7) ◽  
pp. 2059-2075 ◽  
Author(s):  
Guillaume Goyette-Desjardins ◽  
Cynthia Calzas ◽  
Tze Chieh Shiao ◽  
Axel Neubauer ◽  
Jennifer Kempker ◽  
...  
Keyword(s):  
Conjugate Vaccine ◽  
Functional Activity ◽  
Bacterial Infections ◽  
Capsular Polysaccharide ◽  
Streptococcus Suis ◽  
Vaccine Strategy ◽  
Content Type ◽  
Glycoconjugate Vaccine

Streptococcus suisserotype 2 is an encapsulated bacterium and one of the most important bacterial pathogens in the porcine industry. Despite decades of research for an efficient vaccine, none is currently available. Based on the success achieved with other encapsulated pathogens, a glycoconjugate vaccine strategy was selected to elicit opsonizing anti-capsular polysaccharide (anti-CPS) IgG antibodies. In this work, glycoconjugate prototypes were prepared by couplingS. suistype 2 CPS to tetanus toxoid, and the immunological features of the postconjugation preparations were evaluatedin vivo. In mice, experiments evaluating three different adjuvants showed that CpG oligodeoxyribonucleotide (ODN) induces very low levels of anti-CPS IgM antibodies, while the emulsifying adjuvants Stimune and TiterMax Gold both induced high levels of IgGs and IgM. Dose-response trials comparing free CPS with the conjugate vaccine showed that free CPS is nonimmunogenic independently of the dose used, while 25 μg of the conjugate preparation was optimal in inducing high levels of anti-CPS IgGs postboost. With an opsonophagocytosis assay using murine whole blood, sera from immunized mice showed functional activity. Finally, the conjugate vaccine showed immunogenicity and induced protection in a swine challenge model. When conjugated and administered with emulsifying adjuvants,S. suistype 2 CPS is able to induce potent IgM and isotype-switched IgGs in mice and pigs, yielding functional activityin vitroand protection against a lethal challengein vivo, all features of a T cell-dependent response. This study represents a proof of concept for the potential of glycoconjugate vaccines in veterinary medicine applications against invasive bacterial infections.

Exemplar Abstract for Streptococcus suis (ex Elliott 1966) Kilpper-Bälz and Schleifer 1987.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Streptococcus Suis

Exemplar Abstract for Streptococcus suis (ex Elliott 1966) Kilpper-Bälz and Schleifer 1987.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Streptococcus Suis

Exemplar Abstract for Streptococcus suis (ex Elliott 1966) Kilpper-Bälz and Schleifer 1987.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Streptococcus Suis

Exemplar Abstract for Streptococcus suis (ex Elliott 1966) Kilpper-Bälz and Schleifer 1987.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Streptococcus Suis

scholarly journals Use of Proteins Identified through a Functional Genomic Screen To Develop a Protein Subunit Vaccine That Provides Significant Protection against Virulent Streptococcus suis in Pigs

2017 ◽  
Vol 86 (3) ◽  
Author(s):  
Susan L. Brockmeier ◽  
Crystal L. Loving ◽  
Tracy L. Nicholson ◽  
Jinhong Wang ◽  
Sarah E. Peters ◽  
...  
Keyword(s):  
Immune Response ◽  
Vaccine Trial ◽  
Streptococcus Suis ◽  
Protein Subunit ◽  
Content Type ◽  
Degree Of Protection ◽  
Wide Range ◽  
Associated Proteins ◽  
Clinical Protection ◽  
Cellular Immune

ABSTRACT Streptococcus suis is a bacterium that is commonly carried in the respiratory tract and that is also one of the most important invasive pathogens of swine, commonly causing meningitis, arthritis, and septicemia. Due to the existence of many serotypes and a wide range of immune evasion capabilities, efficacious vaccines are not readily available. The selection of S. suis protein candidates for inclusion in a vaccine was accomplished by identifying fitness genes through a functional genomics screen and selecting conserved predicted surface-associated proteins. Five candidate proteins were selected for evaluation in a vaccine trial and administered both intranasally and intramuscularly with one of two different adjuvant formulations. Clinical protection was evaluated by subsequent intranasal challenge with virulent S. suis . While subunit vaccination with the S. suis proteins induced IgG antibodies to each individual protein and a cellular immune response to the pool of proteins and provided substantial protection from challenge with virulent S. suis , the immune response elicited and the degree of protection were dependent on the parenteral adjuvant given. Subunit vaccination induced IgG reactive against different S. suis serotypes, indicating a potential for cross protection.

Study of the relationship between untypable and typable isolates of Streptococcus suis recovered from clinically ill and healthy nursery pigs

2021 ◽  
pp. 109064
Author(s):  
Leann Denich ◽  
Abdolvahab Farzan ◽  
Robert Friendship ◽  
Emily Arndt ◽  
Nicole Ricker ◽  
...  
Keyword(s):  
Streptococcus Suis ◽  
Nursery Pigs ◽  
The Relationship

scholarly journals d-enantiomers of CATH-2 enhance the response of macrophages against Streptococcus suis serotype 2

2021 ◽  
Author(s):  
Roel M. van Harten ◽  
Johanna L.M. Tjeerdsma-van Bokhoven ◽  
Astrid de Greeff ◽  
Melanie D. Balhuizen ◽  
Albert van Dijk ◽  
...  
Keyword(s):  
Streptococcus Suis ◽  
Suis Serotype ◽  
Streptococcus Suis Serotype 2
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