Programming Fluorogenic DNA Probes for Rapid Detection of Steroids

2021 ◽  
Author(s):  
Sasha B. Ebrahimi ◽  
Devleena Samanta ◽  
Benjamin E. Partridge ◽  
Caroline D. Kusmierz ◽  
Ho Fung Cheng ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Dna Probes

Development of DNA probes for the identification of sibling species A of the Anopheles culicifacies (Diptera: Culicidae) complex

1995 ◽  
Vol 85 (3) ◽  
pp. 345-353 ◽  
Author(s):  
Maya B. Gunasekera ◽  
B.G.D.N.K. de Silva ◽  
W. Abeyewickreme ◽  
S.K. Subbarao ◽  
H.G. Nandadasa ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Rapid Detection ◽  
Copy Number ◽  
Blot Hybridization ◽  
Dna Probes ◽  
Anopheles Culicifacies ◽  
Dot Blot ◽  
Dot Blot Hybridization ◽  
Field Investigations ◽  
Highly Repetitive Dna

AbstractThree highly repetitive DNA sequences Rp36, Rp217 and Rp234, have been isolated from Anopheles culicifacies Giles sensu lato. The cloned DNA sequences were found at a higher copy number in species B and C, than in species A of the A. culicifacies complex. These sequences may therefore be used as DNA probes to distinguish species A from the other two species, using a 200-fold dilution of a single mosquito DNA extract in a dot-blot hybridization assay. Rp36 and Rp217 have been completely sequenced. Internal repeats were absent in Rp36. Two related core sequences of 13 and 16 bp were found tandemly repeated in Rp217. These probes enable the rapid detection of species A of A. culicifacies in field investigations.

Programming Fluorogenic DNA Probes for Rapid Detection of Steroids

2021 ◽  
Author(s):  
Sasha B. Ebrahimi ◽  
Devleena Samanta ◽  
Benjamin E. Partridge ◽  
Caroline D. Kusmierz ◽  
Ho Fung Cheng ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Dna Probes

Digoxigenin labelled DNA probes for rapid detection of enterotoxigenic, enteropathogenic and Vero cytotoxin producing Escherichia coli in faecal samples

1992 ◽  
Vol 15 (2) ◽  
pp. 121-127 ◽  
Author(s):  
C.M.A. Rademaker ◽  
M.J.H.M. Wolfhagen ◽  
M. Jansze ◽  
M. Oteman ◽  
A.C. Fluit ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Dna Probes ◽  
Faecal Samples

Search mode for rapid detection of virus particles

1991 ◽  
Vol 49 ◽  
pp. 286-287
Author(s):  
O. E. Bradfute
Keyword(s):  
Electron Microscopy ◽  
Rapid Detection ◽  
Plant Virus ◽  
Low Dose ◽  
Search Mode ◽  
Virus Diseases ◽  
Virus Particles ◽  
Focus Image ◽  
Time Required ◽  
Rapid Switching

Electron microscopy is frequently used in preliminary diagnosis of plant virus diseases by surveying negatively stained preparations of crude extracts of leaf samples. A major limitation of this method is the time required to survey grids when the concentration of virus particles (VPs) is low. A rapid survey of grids for VPs is reported here; the method employs a low magnification, out-of-focus Search Mode similar to that used for low dose electron microscopy of radiation sensitive specimens. A higher magnification, in-focus Confirm Mode is used to photograph or confirm the detection of VPs. Setting up the Search Mode by obtaining an out-of-focus image of the specimen in diffraction (K. H. Downing and W. Chiu, private communications) and pre-aligning the image in Search Mode with the image in Confirm Mode facilitates rapid switching between Modes.

Identification of hepatitis a virus in cell cultures by solid phase immune electron microscopy

1986 ◽  
Vol 44 ◽  
pp. 238-239
Author(s):  
C.D. Humphrey ◽  
T.L. Cromeans ◽  
E.H. Cook ◽  
D.W. Bradley
Keyword(s):  
Electron Microscopy ◽  
Liquid Phase ◽  
Cell Cultures ◽  
Rapid Detection ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Immune Electron Microscopy ◽  
Detection And Identification

There is a variety of methods available for the rapid detection and identification of viruses by electron microscopy as described in several reviews. The predominant techniques are classified as direct electron microscopy (DEM), immune electron microscopy (IEM), liquid phase immune electron microscopy (LPIEM) and solid phase immune electron microscopy (SPIEM). Each technique has inherent strengths and weaknesses. However, in recent years, the most progress for identifying viruses has been realized by the utilization of SPIEM.

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