Unraveling Dual Aggregation‐Induced Emission Behavior in Steric‐Hindrance Photochromic System for Super Resolution Imaging

2019 ◽  
Vol 132 (22) ◽  
pp. 8638-8648 ◽  
Author(s):  
Hong Yang ◽  
Mengqi Li ◽  
Chong Li ◽  
Qianfu Luo ◽  
Ming‐Qiang Zhu ◽  
...  
Keyword(s):  
Steric Hindrance ◽  
Super Resolution ◽  
Aggregation Induced Emission ◽  
Emission Behavior ◽  
Resolution Imaging

Photoswitchable aggregation-induced emission of a dithienylethene–tetraphenylethene conjugate for optical memory and super-resolution imaging

RSC Advances ◽  
2013 ◽  
Vol 3 (23) ◽  
pp. 8967 ◽  
Author(s):  
Chong Li ◽  
Wen-Liang Gong ◽  
Zhe Hu ◽  
Matthew P. Aldred ◽  
Guo-Feng Zhang ◽  
...  
Keyword(s):  
Optical Memory ◽  
Super Resolution ◽  
Aggregation Induced Emission ◽  
Resolution Imaging

scholarly journals Facilely prepared aggregation-induced emission (AIE) nanocrystals with deep-red emission for super-resolution imaging

2022 ◽  
Author(s):  
Ruohan Xu ◽  
Dongfeng Dang ◽  
Zhi Wang ◽  
Yu Zhou ◽  
Yanzi Xu ◽  
...  
Keyword(s):  
Super Resolution ◽  
Biological Imaging ◽  
Fast Method ◽  
High Brightness ◽  
Red Emission ◽  
Aggregation Induced Emission ◽  
Organic Nanocrystals ◽  
Resolution Imaging

Organic nanocrystals (NCs) with high brightness are highly desirable for biological imaging. However, the preparation of NCs in a facile and fast method is still challenging. Herein, aggregation-induced emission (AIE)...

scholarly journals Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidin

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Ingrid Chamma ◽  
Mathieu Letellier ◽  
Corey Butler ◽  
Béatrice Tessier ◽  
Kok-Hong Lim ◽  
...  
Keyword(s):  
High Resolution ◽  
Membrane Proteins ◽  
Steric Hindrance ◽  
Transmembrane Protein ◽  
Super Resolution ◽  
Leucine Rich Repeat ◽  
Versatile Tool ◽  
Resolution Imaging ◽  
Dual Colour ◽  
Specific Labelling

Abstract The advent of super-resolution imaging (SRI) has created a need for optimized labelling strategies. We present a new method relying on fluorophore-conjugated monomeric streptavidin (mSA) to label membrane proteins carrying a short, enzymatically biotinylated tag, compatible with SRI techniques including uPAINT, STED and dSTORM. We demonstrate efficient and specific labelling of target proteins in confined intercellular and organotypic tissues, with reduced steric hindrance and no crosslinking compared with multivalent probes. We use mSA to decipher the dynamics and nanoscale organization of the synaptic adhesion molecules neurexin-1β, neuroligin-1 (Nlg1) and leucine-rich-repeat transmembrane protein 2 (LRRTM2) in a dual-colour configuration with GFP nanobody, and show that these proteins are diffusionally trapped at synapses where they form apposed trans-synaptic adhesive structures. Furthermore, Nlg1 is dynamic, disperse and sensitive to synaptic stimulation, whereas LRRTM2 is organized in compact and stable nanodomains. Thus, mSA is a versatile tool to image membrane proteins at high resolution in complex live environments, providing novel information about the nano-organization of biological structures.

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