High-Throughput Design of Biocompatible Enzyme-Based Hydrogel Microparticles with Autonomous Movement

2018 ◽  
Vol 130 (31) ◽  
pp. 9962-9965 ◽  
Author(s):  
Shauni Keller ◽  
Serena P. Teora ◽  
Guo Xun Hu ◽  
Marlies Nijemeisland ◽  
Daniela A. Wilson
Keyword(s):  
High Throughput ◽  
Hydrogel Microparticles ◽  
Autonomous Movement

High-Throughput Design of Biocompatible Enzyme-Based Hydrogel Microparticles with Autonomous Movement

2018 ◽  
Vol 57 (31) ◽  
pp. 9814-9817 ◽  
Author(s):  
Shauni Keller ◽  
Serena P. Teora ◽  
Guo Xun Hu ◽  
Marlies Nijemeisland ◽  
Daniela A. Wilson
Keyword(s):  
High Throughput ◽  
Hydrogel Microparticles ◽  
Autonomous Movement

scholarly journals High-throughput flow alignment of barcoded hydrogel microparticles

Lab on a Chip ◽  
2009 ◽  
Vol 9 (21) ◽  
pp. 3100 ◽  
Author(s):  
Stephen C. Chapin ◽  
Daniel C. Pregibon ◽  
Patrick S. Doyle
Keyword(s):  
High Throughput ◽  
Hydrogel Microparticles ◽  
Flow Alignment

scholarly journals Hydrogel Microparticles Functionalized with Engineered Escherichia coli as Living Lactam Biosensors

Sensors ◽  
2019 ◽  
Vol 19 (24) ◽  
pp. 5556 ◽  
Author(s):  
Conghui Ma ◽  
Jie Li ◽  
Boyin Zhang ◽  
Chenxi Liu ◽  
Jingwei Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Flow Cytometry ◽  
Metabolic Engineering ◽  
High Throughput ◽  
Droplet Microfluidics ◽  
Dependent Manner ◽  
Hydrogel Microparticles ◽  
Commodity Chemicals ◽  
Hydrogel Microparticle ◽  
Dose Dependent

Recently there has been an increasing need for synthesizing valued chemicals through biorefineries. Lactams are an essential family of commodity chemicals widely used in the nylon industry with annual production of millions of tons. The bio-production of lactams can substantially benefit from high-throughput lactam sensing strategies for lactam producer screening. We present here a robust and living lactam biosensor that is directly compatible with high-throughput analytical means. The biosensor is a hydrogel microparticle encapsulating living microcolonies of engineered lactam-responsive Escherichia coli. The microparticles feature facile and ultra-high throughput manufacturing of up to 10,000,000 per hour through droplet microfluidics. We show that the biosensors can specifically detect major lactam species in a dose-dependent manner, which can be quantified using flow cytometry. The biosensor could potentially be used for high-throughput metabolic engineering of lactam biosynthesis.

scholarly journals Massively parallel encapsulation of single cells with structured microparticles and secretion-based flow sorting

2020 ◽  
Author(s):  
Joseph de Rutte ◽  
Robert Dimatteo ◽  
Mark van Zee ◽  
Robert Damoiseaux ◽  
Dino Di Carlo
Keyword(s):  
High Throughput ◽  
Cell Attachment ◽  
Single Cells ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Cell Secretion ◽  
High Throughput Analysis ◽  
Ovary Cells ◽  
Hydrogel Microparticles ◽  
Specialized Equipment

AbstractTechniques to analyze and sort single cells based on secreted products have the potential to transform our understanding of cellular biology as well as accelerate the development of next generation cell and antibody therapies. However, secretions are rapidly transported away from cells, such that specialized equipment and expertise has been required to compartmentalize cells and capture their secretions. Herein we demonstrate the use of cavity-containing hydrogel microparticles to perform functional single-cell secretion analysis and sorting using only commonly accessible lab infrastructure. These microparticles act as a solid support which facilitates cell attachment, templates formation of uniform aqueous compartments which prevent cross-talk between cells, and captures secreted proteins. Using this platform we demonstrate high-throughput analysis and sorting of Chinese Hamster Ovary cells based on their relative production of human IgG using commercially available flow sorters. Microparticles are easily distributed and used, democratizing access to high-throughput functional cell screening.

Engineered cell-laden alginate microparticles for 3D culture

2021 ◽  
Author(s):  
Bumseok Namgung ◽  
Kalpana Ravi ◽  
Pooja Prathyushaa Vikraman ◽  
Shiladitya Sengupta ◽  
Hae Lin Jang
Keyword(s):  
High Throughput ◽  
Cellular Structure ◽  
3D Culture ◽  
Cell Encapsulation ◽  
Alginate Hydrogel ◽  
Encapsulated Cells ◽  
Tissue Microenvironment ◽  
Hydrogel Microparticles ◽  
Cell Based Therapy ◽  
Screening Platform

Advanced microfabrication technologies and biocompatible hydrogel materials facilitate the modeling of 3D tissue microenvironment. Encapsulation of cells in hydrogel microparticles offers an excellent high-throughput platform for investigating multicellular interaction with their surrounding microenvironment. Compartmentalized microparticles support formation of various unique cellular structures. Alginate has emerged as one of the most dominant hydrogel materials for cell encapsulation owing to its cytocompatibility, ease of gelation, and biocompatibility. Alginate hydrogel provides a permeable physical boundary to the encapsulated cells and develops an easily manageable 3D cellular structure. The interior structure of alginate hydrogel can further regulate the spatiotemporal distribution of the embedded cells. This review provides a specific overview of the representative engineering approaches to generate various structures of cell-laden alginate microparticles in a uniform and reproducible manner. Capillary nozzle systems, microfluidic droplet systems, and non-chip based high-throughput microfluidic systems are highlighted for developing well-regulated cellular structure in alginate microparticles to realize potential drug screening platform and cell-based therapy. We conclude with the discussion of current limitations and future directions for realizing the translation of this technology to the clinic.

Sensitive, quantitative, and high-throughput detection of angiogenic markers using shape-coded hydrogel microparticles

The Analyst ◽  
2015 ◽  
Vol 140 (13) ◽  
pp. 4530-4539 ◽  
Author(s):  
Mohammad Ali Al-Ameen ◽  
Ji Li ◽  
David G. Beer ◽  
Gargi Ghosh
Keyword(s):  
High Throughput ◽  
Multiplexed Detection ◽  
Hydrogel Microparticles ◽  
Spectral Overlap ◽  
High Throughput Detection ◽  
Angiogenic Markers ◽  
Multiplexed Analysis

Demonstration of the application of shape coded hydrogel microparticles for multiplexed detection of angiogenic molecules. Utilization of single fluorophore eliminates the spectral overlap associated with microparticle based multiplexed analysis.

154: Integration of TPSA and High-Throughput Mass Spectrometry Data Improves Prostate Cancer Prediction

2007 ◽  
Vol 177 (4S) ◽  
pp. 52-53
Author(s):  
Stefano Ongarello ◽  
Eberhard Steiner ◽  
Regina Achleitner ◽  
Isabel Feuerstein ◽  
Birgit Stenzel ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Mass Spectrometry ◽  
High Throughput ◽  
Mass Spectrometry Data ◽  
Cancer Prediction
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