Syntheses and Immunological Evaluation of Self‐Adjuvanting Clustered N ‐Acetyl and N ‐Propionyl Sialyl‐Tn Combined with a T‐helper Cell Epitope as Antitumor Vaccine Candidates

2018 ◽  
Vol 130 (27) ◽  
pp. 8351-8356 ◽  
Author(s):  
Tsung‐Che Chang ◽  
Yoshiyuki Manabe ◽  
Yukari Fujimoto ◽  
Shino Ohshima ◽  
Yoshie Kametani ◽  
...  
Keyword(s):  
Cell Epitope ◽  
T Helper Cell ◽  
Helper Cell ◽  
T Helper ◽  
Vaccine Candidates ◽  
Antitumor Vaccine ◽  
Immunological Evaluation ◽  
Sialyl Tn

scholarly journals A Polypeptide of Tumor-Associated Antigen L6 with Intrinsic Adjuvant Activity Enhances Antitumor Immunity

Vaccines ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 620
Author(s):  
Yuh-Pyng Sher ◽  
Kit Man Chai ◽  
Wen-Ching Chen ◽  
Kuan-Yin Shen ◽  
I-Hua Chen ◽  
...  
Keyword(s):  
Immune Responses ◽  
Cancer Vaccine ◽  
Antitumor Immunity ◽  
Cell Epitope ◽  
Specific Treatment ◽  
T Helper Cell ◽  
Helper Cell ◽  
Peptide Vaccines ◽  
T Helper ◽  
Tumor Associated Antigen

Peptide vaccines are safe, and aim to elicit and expand tumor-specific immunity so as to eradicate tumors. However, achieving strong and long-lasting anti-tumor immunity with peptide vaccines for the antigen-specific treatment of cancer is challenging, in part because their efficacy depends on strong adjuvants or immunomodulators. We approached this problem by conjugating an epitope-based cancer vaccine with a lipidated sequence (an immunomodulator) to elicit a strong immune response. Lipidated and non-lipidated polyepitope proteins were generated that contained the universal T helper cell epitope (pan-DR), B cell epitopes, and the extended loop sequence of extracellular domain 2 of tumor-associated antigen L6 (TAL6). We show that the lipidated polyepitope cancer vaccine can activate bone marrow-derived dendritic cells, and trigger effective antigen-specific antibody and T helper cell responses, more effectively than the non-lipidated vaccine. Moreover, potent T cell immune responses were elicited in mice inoculated with the lipidated polyepitope cancer vaccine, providing protective antitumor immunity in mice bearing TAL6 tumors. Our study demonstrates that a lipidated polyepitope cancer vaccine could be employed to generate potent anti-tumor immune responses, including humoral and cellular immunity, which could be beneficial in the treatment of TAL6+ cancer.

Expression and purification of a soluble B lymphocyte stimulator mutant modified with the T-helper cell epitope

2006 ◽  
Vol 28 (20) ◽  
pp. 1649-1654
Author(s):  
Huiguang Gao ◽  
Weiling Fu ◽  
Rongfen Li ◽  
Linfeng Chen ◽  
Qing Ji ◽  
...  
Keyword(s):  
B Lymphocyte ◽  
Cell Epitope ◽  
T Helper Cell ◽  
Helper Cell ◽  
T Helper ◽  
Expression And Purification ◽  
B Lymphocyte Stimulator

Enhanced T Helper Cell Epitope Peptide Vaccines for Melanoma

2004 ◽  
Vol 27 (6) ◽  
pp. S54-S55
Author(s):  
Nikoletta Kallinteris ◽  
Wu Shuzhen ◽  
Lu Xueqing ◽  
Xu Minzhen ◽  
Eric von Hofe
Keyword(s):  
Cell Epitope ◽  
T Helper Cell ◽  
Helper Cell ◽  
Peptide Vaccines ◽  
T Helper ◽  
Epitope Peptide

scholarly journals Gamma Interferon-Dependent Protection of the Mouse Upper Respiratory Tract following Parenteral Immunization with a Respiratory Syncytial Virus G Protein Fragment

2002 ◽  
Vol 76 (20) ◽  
pp. 10203-10210 ◽  
Author(s):  
Helene Plotnicky-Gilquin ◽  
Dominique Cyblat-Chanal ◽  
Jean-Pierre Aubry ◽  
Thierry Champion ◽  
Alain Beck ◽  
...  
Keyword(s):  
G Protein ◽  
Cell Epitope ◽  
T Helper Cell ◽  
Helper Cell ◽  
Amino Acid Residues ◽  
Upper Respiratory Tract ◽  
T Helper ◽  
Parenteral Immunization ◽  
Ifn Γ ◽  
Syncytial Virus

ABSTRACT The protective mechanisms induced in the mouse upper respiratory tract (URT) after intraperitoneal immunization with G2Na, a recombinant respiratory syncytial virus (RSV) G protein fragment (amino acid residues 130 to 230), were investigated. This protection was recently shown to be mediated by CD4+ T cells and to be critically dependent on the cysteines and amino acids 193 and 194 (H. Plotnicky-Gilquin, A. Robert, L. Chevalet, J.-F. Haeuw, A. Beck, J.-Y. Bonnefoy, C. Brandt, C.-A. Siegrist, T. N. Nguyen, and U. F. Power, J. Virol. 74:3455-3463, 2000). On G2Na, we identified a domain (amino acid residues 182 to 198) responsible for the T-helper-cell activity. This region coincided with a peptide designed AICK (residues 184 to 198) which includes the previously identified murine and human T-helper-cell epitope on the native G protein (P. W. Tebbey, M. Hagen, and G. E. Hancock, J. Exp. Med. 188:1967-1972, 1998). Immunization with AICK, in alum or complete Freund's adjuvant, significantly reduced nasal RSV titers in normal BALB/c mice. However, although lung protection was induced, in contrast to the case with live RSV, neither AICK nor G2Na was able to prevent nasal infection in gamma interferon (IFN-γ)-knockout mice. Anti-IFN-γ neutralizing antibodies partially inhibited URT protection after administration to G2Na-immunized BALB/c mice. Furthermore, while purified CD4+ T cells from BALB/c mice immunized with G2Na or AICK significantly reduced lung and nasal infection of naive recipient mice after adoptive transfer, the cells from IFN-γ-knockout mice had no effect. Together, these results demonstrated for the first time that the T-helper-cell epitope of RSV G protein induces URT protection in mice after parenteral immunization through a Th1-type, IFN-γ-dependent mechanism.

T helper cell epitope of rabies virus nucleoprotein defined by tri- and tetrapeptides

1991 ◽  
Vol 21 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Hildegund C. J. Ertl ◽  
Bernhard Dietzschold ◽  
Laszlo Otvos
Keyword(s):  
Rabies Virus ◽  
Cell Epitope ◽  
T Helper Cell ◽  
Helper Cell ◽  
T Helper

scholarly journals Identification of a T-helper cell epitope on the rotavirus VP6 protein.

1997 ◽  
Vol 71 (1) ◽  
pp. 419-426 ◽  
Author(s):  
D M Baños ◽  
S Lopez ◽  
C F Arias ◽  
F R Esquivel
Keyword(s):  
Cell Epitope ◽  
T Helper Cell ◽  
Helper Cell ◽  
T Helper ◽  
Rotavirus Vp6
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