scholarly journals Titelbild: Intracellular Delivery of Native Proteins Facilitated by Cell-Penetrating Poly(disulfide)s (Angew. Chem. 6/2018)

2018 ◽  
Vol 130 (6) ◽  
pp. 1435-1435
Author(s):  
Linghui Qian ◽  
Jiaqi Fu ◽  
Peiyan Yuan ◽  
Shubo Du ◽  
Wei Huang ◽  
...  
Keyword(s):  
Intracellular Delivery ◽  
Native Proteins ◽  
Cell Penetrating

Intracellular Delivery of Native Proteins Facilitated by Cell-Penetrating Poly(disulfide)s

2018 ◽  
Vol 130 (6) ◽  
pp. 1548-1552 ◽  
Author(s):  
Linghui Qian ◽  
Jiaqi Fu ◽  
Peiyan Yuan ◽  
Shubo Du ◽  
Wei Huang ◽  
...  
Keyword(s):  
Intracellular Delivery ◽  
Native Proteins ◽  
Cell Penetrating

Intracellular Delivery of Native Proteins Facilitated by Cell-Penetrating Poly(disulfide)s

2018 ◽  
Vol 57 (6) ◽  
pp. 1532-1536 ◽  
Author(s):  
Linghui Qian ◽  
Jiaqi Fu ◽  
Peiyan Yuan ◽  
Shubo Du ◽  
Wei Huang ◽  
...  
Keyword(s):  
Intracellular Delivery ◽  
Native Proteins ◽  
Cell Penetrating

scholarly journals Cover Picture: Intracellular Delivery of Native Proteins Facilitated by Cell-Penetrating Poly(disulfide)s (Angew. Chem. Int. Ed. 6/2018)

2018 ◽  
Vol 57 (6) ◽  
pp. 1421-1421
Author(s):  
Linghui Qian ◽  
Jiaqi Fu ◽  
Peiyan Yuan ◽  
Shubo Du ◽  
Wei Huang ◽  
...  
Keyword(s):  
Intracellular Delivery ◽  
Native Proteins ◽  
Cell Penetrating

Porous Silicon–Cell Penetrating Peptide Hybrid Nanocarrier for Intracellular Delivery of Oligonucleotides

2013 ◽  
Vol 11 (2) ◽  
pp. 382-390 ◽  
Author(s):  
Jussi Rytkönen ◽  
Piret Arukuusk ◽  
Wujun Xu ◽  
Kaido Kurrikoff ◽  
Ülo Langel ◽  
...  
Keyword(s):  
Porous Silicon ◽  
Intracellular Delivery ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
Silicon Cell

scholarly journals Novel human-derived cell-penetrating peptides for specific subcellular delivery of therapeutic biomolecules

2005 ◽  
Vol 390 (2) ◽  
pp. 407-418 ◽  
Author(s):  
Catherine de Coupade ◽  
Antonio Fittipaldi ◽  
Vanessa Chagnas ◽  
Matthieu Michel ◽  
Sophie Carlier ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Mammalian Cells ◽  
Heparan Sulphate ◽  
Intracellular Delivery ◽  
Membrane Lipid ◽  
Cell Penetrating Peptides ◽  
Heparin Binding ◽  
Independent Manner ◽  
Cell Penetrating

Short peptide sequences that are able to transport molecules across the cell membrane have been developed as tools for intracellular delivery of therapeutic molecules. This work describes a novel family of cell-penetrating peptides named Vectocell® peptides [also termed DPVs (Diatos peptide vectors)]. These peptides, originating from human heparin binding proteins and/or anti-DNA antibodies, once conjugated to a therapeutic molecule, can deliver the molecule to either the cytoplasm or the nucleus of mammalian cells. Vectocell® peptides can drive intracellular delivery of molecules of varying molecular mass, including full-length active immunoglobulins, with efficiency often greater than that of the well-characterized cell-penetrating peptide Tat. The internalization of Vectocell® peptides has been demonstrated to occur in both adherent and suspension cell lines as well as in primary cells through an energy-dependent endocytosis process, involving cell-membrane lipid rafts. This endocytosis occurs after binding of the cell-penetrating peptides to extracellular heparan sulphate proteoglycans, except for one particular peptide (DPV1047) that partially originates from an anti-DNA antibody and is internalized in a caveolar independent manner. These new therapeutic tools are currently being developed for intracellular delivery of a number of active molecules and their potentiality for in vivo transduction investigated.

scholarly journals A boronic acid–rich dendrimer with robust and unprecedented efficiency for cytosolic protein delivery and CRISPR-Cas9 gene editing

2019 ◽  
Vol 5 (6) ◽  
pp. eaaw8922 ◽  
Author(s):  
Chongyi Liu ◽  
Tao Wan ◽  
Hui Wang ◽  
Song Zhang ◽  
Yuan Ping ◽  
...  
Keyword(s):  
Boronic Acid ◽  
Protein Delivery ◽  
High Efficiency ◽  
Intracellular Delivery ◽  
Cytosolic Protein ◽  
Native Proteins ◽  
Cytosolic Delivery ◽  
Cas9 Protein ◽  
Cargo Proteins ◽  
Positively Charged

Cytosolic protein delivery is of central importance for the development of protein-based biotechnologies and therapeutics; however, efficient intracellular delivery of native proteins remains a challenge. Here, we reported a boronic acid–rich dendrimer with unprecedented efficiency for cytosolic delivery of native proteins. The dendrimer could bind with both negatively and positively charged proteins and efficiently delivered 13 cargo proteins into the cytosol of living cells. All the delivered proteins kept their bioactivities after cytosolic delivery. The dendrimer ensures efficient intracellular delivery of Cas9 protein into various cell lines and showed high efficiency in CRISPR-Cas9 genome editing. The rationally designed boronic acid–rich dendrimer permits the development of an efficient platform with high generality for the delivery of native proteins.

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