N-Heterocyclic Carbene Boranes as Reactive Oxygen Species-Responsive Materials: Application to the Two-Photon Imaging of Hypochlorous Acid in Living Cells and Tissues

2018 ◽  
Vol 130 (6) ◽  
pp. 1583-1587 ◽  
Author(s):  
Yen Leng Pak ◽  
Sang Jun Park ◽  
Di Wu ◽  
BoHyun Cheon ◽  
Hwan Myung Kim ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Hypochlorous Acid ◽  
Reactive Oxygen ◽  
Living Cells ◽  
Oxygen Species ◽  
Responsive Materials ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals Oxalate-curcumin–based probe for micro- and macroimaging of reactive oxygen species in Alzheimer’s disease

2017 ◽  
Vol 114 (47) ◽  
pp. 12384-12389 ◽  
Author(s):  
Jian Yang ◽  
Xueli Zhang ◽  
Peng Yuan ◽  
Jing Yang ◽  
Yungen Xu ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Reactive Oxygen Species ◽  
Alzheimer's Disease ◽  
Reactive Oxygen ◽  
Amyloid Angiopathy ◽  
Oxygen Species ◽  
Two Photon ◽  
Nirf Imaging ◽  
Photon Imaging ◽  
Two Photon Imaging

Alzheimer’s disease (AD) is an irreversible neurodegenerative disorder that has a progression that is closely associated with oxidative stress. It has long been speculated that the reactive oxygen species (ROS) level in AD brains is much higher than that in healthy brains. However, evidence from living beings is scarce. Inspired by the “chemistry of glow stick,” we designed a near-IR fluorescence (NIRF) imaging probe, termed CRANAD-61, for sensing ROS to provide evidence at micro- and macrolevels. In CRANAD-61, an oxalate moiety was utilized to react with ROS and to consequentially produce wavelength shifting. Our in vitro data showed that CRANAD-61 was highly sensitive and rapidly responsive to various ROS. On reacting with ROS, its excitation and emission wavelengths significantly shifted to short wavelengths, and this shifting could be harnessed for dual-color two-photon imaging and transformative NIRF imaging. In this report, we showed that CRANAD-61 could be used to identify “active” amyloid beta (Aβ) plaques and cerebral amyloid angiopathy (CAA) surrounded by high ROS levels with two-photon imaging (microlevel) and to provide relative total ROS concentrations in AD brains via whole-brain NIRF imaging (macrolevel). Lastly, we showed that age-related increases in ROS levels in AD brains could be monitored with our NIRF imaging method. We believe that our imaging with CRANAD-61 could provide evidence of ROS at micro- and macrolevels and could be used for monitoring ROS changes under various AD pathological conditions and during drug treatment.

Development of an endoplasmic reticulum-targeting fluorescent probe for the two-photon imaging of hypochlorous acid (HClO) in living cells

2019 ◽  
Vol 11 (35) ◽  
pp. 4450-4455 ◽  
Author(s):  
Wenhui Song ◽  
Baoli Dong ◽  
Yaru Lu ◽  
Xiuqi Kong ◽  
Abdul Hadi Mehmood ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Endoplasmic Reticulum ◽  
Cell Death ◽  
Fluorescent Probe ◽  
Hypochlorous Acid ◽  
Living Cells ◽  
Oxygen Species ◽  
Cell Dysfunction ◽  
Two Photon ◽  
Endoplasmic Reticulum Targeting

As an important reactive oxygen species, hypochlorous acid (HClO) could irreversibly destroy Ca2+-ATPase to inhibit Ca2+ transport in the endoplasmic reticulum (ER), which may eventually lead to cell dysfunction and cell death.

A two-photon fluorescent probe for ratiometric imaging of endogenous hypochlorous acid in live cells and tissues

2017 ◽  
Vol 53 (78) ◽  
pp. 10800-10803 ◽  
Author(s):  
Yong Woong Jun ◽  
Sourav Sarkar ◽  
Subhankar Singha ◽  
Ye Jin Reo ◽  
Hye Rim Kim ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Fluorescent Probe ◽  
Hypochlorous Acid ◽  
Reactive Oxygen ◽  
Live Cells ◽  
Oxygen Species ◽  
Two Photon ◽  
Ratiometric Imaging ◽  
In Cells

A fluorescent probe enables two-photon ratiometric imaging of endogenous hypochlorous acid, a reactive oxygen species, in cells and tissues.

Hydrogen peroxide-responsive AIE probe for imaging-guided organelle targeting and photodynamic cancer cell ablation

2021 ◽  
Author(s):  
Qian Wu ◽  
Youmei Li ◽  
Ying Li ◽  
Dong Wang ◽  
Ben Zhong Tang
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Cancer Cell ◽  
Reactive Oxygen ◽  
Living Cells ◽  
Oxygen Species ◽  
Cell Ablation ◽  
Selective Monitoring ◽  
Organelle Targeting

Hydrogen peroxide (H2O2), as one kind of key reactive oxygen species (ROS), is mainly produced endogenously primarily in the mitochondria. The selective monitoring of H2O2 in living cells is of...

Colorimetric and Fluorescent Probe for Hypochlorite with High Selectivity

2013 ◽  
Vol 295-298 ◽  
pp. 475-478 ◽  
Author(s):  
Zhi Xiang Han ◽  
Ming Hui Du ◽  
Guo Xi Liang ◽  
Xiang Yang Wu
Keyword(s):  
Reactive Oxygen Species ◽  
Aqueous Solution ◽  
Detection Limit ◽  
Fluorescent Probe ◽  
Rhodamine B ◽  
High Selectivity ◽  
Reactive Oxygen ◽  
Living Cells ◽  
Oxygen Species ◽  
Turn On

Rhodamine B thiohydrazide (RBS) was firstly employed as turn-on fluorescent probe for hypochlorite in aqueous solution and living cells. It exhibits a stable response to hypochlorite from 1.0×10-6to 1.0×10-5M with a detection limit of 3.3×10-7M. The response of this probe to hypochlorite is fast and highly selective compared with other reactive oxygen species (such as.OH,1O2, H2O2) and other common anions (such as X-, ClO2-, ClO4-, NO3-, NO2-, OH-, Ac-, CO32-, SO42-).

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