Supramolecular Nanofibrillar Thermoreversible Hydrogel for Growth and Release of Cancer Spheroids

2016 ◽  
Vol 129 (22) ◽  
pp. 6179-6183 ◽  
Author(s):  
Yunfeng Li ◽  
Nancy Khuu ◽  
Albert Gevorkian ◽  
Sharon Sarjinsky ◽  
Heloise Therien-Aubin ◽  
...  
Keyword(s):  
Thermoreversible Hydrogel ◽  
Cancer Spheroids

Supramolecular Nanofibrillar Thermoreversible Hydrogel for Growth and Release of Cancer Spheroids

2016 ◽  
Vol 56 (22) ◽  
pp. 6083-6087 ◽  
Author(s):  
Yunfeng Li ◽  
Nancy Khuu ◽  
Albert Gevorkian ◽  
Sharon Sarjinsky ◽  
Heloise Therien-Aubin ◽  
...  
Keyword(s):  
Thermoreversible Hydrogel ◽  
Cancer Spheroids

scholarly journals Extracellular vesicles derived from ascitic fluid enhance growth and migration of ovarian cancer cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Aparna Mitra ◽  
Kyoko Yoshida-Court ◽  
Travis N. Solley ◽  
Megan Mikkelson ◽  
Chi Lam Au Yeung ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Extracellular Vesicles ◽  
Ovarian Cancer Cells ◽  
High Grade ◽  
Serous Ovarian Carcinoma ◽  
Advanced Stages ◽  
And Migration ◽  
Cancer Spheroids ◽  
Omental Fat ◽  
Low Expression

AbstractOvarian cancer is associated with a high mortality rate due to diagnosis at advanced stages. Dissemination often occurs intraperitoneally within the ascites fluid. The microenvironment can support dissemination through several mechanisms. One potential ascites factor which may mediate dissemination are EVs or extracellular vesicles that can carry information in the form of miRNAs, proteins, lipids, and act as mediators of cellular communication. We present our observations on EVs isolated from ascitic supernatants from patients diagnosed with high grade serous ovarian carcinoma in augmenting motility, growth, and migration towards omental fat. MicroRNA profiling of EVs from malignant ascitic supernatant demonstrates high expression of miR 200c-3p, miR18a-5p, miR1246, and miR1290 and low expression of miR 100- 5p as compared to EVs isolated from benign ascitic supernatant. The migration of ovarian cancer spheroids towards omental fat is enhanced in the presence of malignant ascitic EVs. Gene expression of these cells showed increased expression of ZBED2, ZBTB20, ABCC3, UHMK1, and low expression of Transgelin and MARCKS. We present evidence that ovarian ascitic EVs increase the growth of ovarian cancer spheroids through miRNAs.

scholarly journals An integrated framework for quantifying immune-tumour interactions in a 3D co-culture model

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Gheed Al-Hity ◽  
FengWei Yang ◽  
Eduard Campillo-Funollet ◽  
Andrew E. Greenstein ◽  
Hazel Hunt ◽  
...  
Keyword(s):  
Immune System ◽  
Immune Cell ◽  
In Vitro Models ◽  
Proof Of Concept ◽  
Culture Model ◽  
Spheroid Growth ◽  
Confocal Images ◽  
Cancer Spheroids

AbstractInvestigational in vitro models that reflect the complexity of the interaction between the immune system and tumours are limited and difficult to establish. Herein, we present a platform to study the tumour-immune interaction using a co-culture between cancer spheroids and activated immune cells. An algorithm was developed for analysis of confocal images of the co-culture to evaluate the following quantitatively; immune cell infiltration, spheroid roundness and spheroid growth. As a proof of concept, the effect of the glucocorticoid stress hormone, cortisol was tested on 66CL4 co-culture model. Results were comparable to 66CL4 syngeneic in vivo mouse model undergoing psychological stress. Furthermore, administration of glucocorticoid receptor antagonists demonstrated the use of this model to determine the effect of treatments on the immune-tumour interplay. In conclusion, we provide a method of quantifying the interaction between the immune system and cancer, which can become a screening tool in immunotherapy design.

scholarly journals Growth of confined cancer spheroids: a combined experimental and mathematical modelling approach

2013 ◽  
Vol 5 (3) ◽  
pp. 597 ◽  
Author(s):  
D. Loessner ◽  
J. A. Flegg ◽  
H. M. Byrne ◽  
J. A. Clements ◽  
D. W. Hutmacher
Keyword(s):  
Mathematical Modelling ◽  
Cancer Spheroids ◽  
Modelling Approach

scholarly journals A comprehensive study on 2D, 3D and solid tumor environment to explore a multifunctional biogenic nanoconjugate

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
B. S. Unnikrishnan ◽  
G. U. Preethi ◽  
T. T. Sreelekha
Keyword(s):  
Drug Delivery ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Tumor Burden ◽  
Oxide Nanoparticles ◽  
Cell Internalization ◽  
Tumor Environment ◽  
D Cell ◽  
Cancer Spheroids ◽  
Comprehensive Study

AbstractEmergence of nanotechnology created a drastic change in the field of cancer therapy due to their unique features in drug delivery and imaging. Polysaccharide based nanoparticles have received extensive attention in recent years as promising nanoparticle mediated drug delivery systems. Polysaccharides are endorsed with versatile merits including high drug encapsulation efficiency, efficient drug protection against chemical or enzymatic degradation, unique ability to create a controlled release and cellular internalization. In the current study, we have fabricated doxorubicin-loaded carboxymethylated PST001 coated iron oxide nanoparticles (DOX@CM-PST-IONPs) for better management of cancer. CM-PST coated iron oxide nanoparticles co-encapsulated with chemotherapeutic drug doxorubicin, can be utilized for targeted drug delivery. Biocompatible and non-toxic nanoconjugates was found to be effective in both 2-D and 3-D cell culture system with efficient cancer cell internalization. The bench-marked potential of CM-PIONPs to produce reactive oxygen species makes it a noticeable drug delivery system to compact neoplasia. These nanoconjugates can lay concrete on a better way for the elimination of cancer spheroids and tumor burden.

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