Reactive Oxygen Species Play an Important Role in the Bactericidal Activity of Quinolone Antibiotics

2016 ◽  
Vol 128 (7) ◽  
pp. 2443-2446
Author(s):  
Jithesh Kottur ◽  
Deepak T. Nair
Keyword(s):  
Reactive Oxygen Species ◽  
Bactericidal Activity ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Quinolone Antibiotics

scholarly journals Phage-Antibiotic Synergy via Delayed Lysis

2018 ◽  
Vol 84 (22) ◽  
Author(s):  
Minjin Kim ◽  
Yunyeol Jo ◽  
Yoon Jung Hwang ◽  
Hye Won Hong ◽  
Sung Sik Hong ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Underlying Mechanism ◽  
Host Cells ◽  
Oxygen Species ◽  
Particle Assembly ◽  
Content Type ◽  
Host Membrane ◽  
Quinolone Antibiotics ◽  
Phage Production

ABSTRACTWhen phages infect bacteria cultured in the presence of sublethal doses of antibiotics, the sizes of the phage plaques are significantly increased. This phenomenon is known as phage-antibiotic synergy (PAS). In this study, the observation of PAS was extended to a wide variety of bacterium-phage pairs using different classes of antibiotics. PAS was shown in both Gram-positive and Gram-negative bacteria. Cells stressed with β-lactam antibiotics filamented or swelled extensively, resulting in an increase in phage production. PAS was also sometimes observed in the presence of other classes of antibiotics with or without bacterial filamentation. The addition of antibiotics inducedrecAexpression in various bacteria, but arecAdeletion mutant strain ofEscherichia colialso showed filamentation and PAS in the presence of quinolone antibiotics. The phage adsorption efficiency did not change in the presence of the antibiotics when the cell surfaces were enlarged as they filamented. Increases in the production of phage DNA and mRNAs encoding phage proteins were observed in these cells, with only a limited increase in protein production. The data suggest that PAS is the product of a prolonged period of particle assembly due to delayed lysis. The increase in the cell surface area far exceeded the increase in phage holin production in the filamented host cells, leading to a relatively limited availability of intracellular holins for aggregating and forming holes in the host membrane. Reactive oxygen species (ROS) stress also led to an increased production of phages, while heat stress resulted in only a limited increase in phage production.IMPORTANCEPhage-antibiotic synergy (PAS) has been reported for a decade, but the underlying mechanism has never been vigorously investigated. This study shows the presence of PAS from a variety of phage-bacterium-antibiotic pairings. We show that increased phage production resulted directly from a lysis delay caused by the relative shortage of holin in filamented bacterial hosts in the presence of sublethal concentrations of stress-inducing substances, such as antibiotics and reactive oxygen species (ROS).

Bactericidal activity of Ag–zeolite mediated by reactive oxygen species under aerated conditions

2002 ◽  
Vol 92 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Yoshihiro Inoue ◽  
Masanobu Hoshino ◽  
Hiroo Takahashi ◽  
Tomoko Noguchi ◽  
Tomomi Murata ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Bactericidal Activity ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals Mode of Bactericidal Action of Silver Zeolite and Its Comparison with That of Silver Nitrate

2003 ◽  
Vol 69 (7) ◽  
pp. 4278-4281 ◽  
Author(s):  
Yoshinobu Matsumura ◽  
Kuniaki Yoshikata ◽  
Shin-ichi Kunisaki ◽  
Tetsuaki Tsuchido
Keyword(s):  
Reactive Oxygen Species ◽  
Silver Nitrate ◽  
Bactericidal Activity ◽  
Bacterial Cell ◽  
Reactive Oxygen ◽  
Silver Ion ◽  
Inorganic Salts ◽  
Oxygen Species ◽  
Bactericidal Action ◽  
Silver Zeolite

ABSTRACT The properties of the bactericidal action of silver zeolite as affected by inorganic salts and ion chelators were similar to those of silver nitrate. The results suggest that the contact of the bacterial cell with silver zeolite, the consequent transfer of silver ion to the cell, and the generation of reactive oxygen species in the cell are involved in the bactericidal activity of silver zeolite.

scholarly journals Kinases Mst1 and Mst2 positively regulate phagocytic induction of reactive oxygen species and bactericidal activity

2015 ◽  
Vol 16 (11) ◽  
pp. 1142-1152 ◽  
Author(s):  
Jing Geng ◽  
Xiufeng Sun ◽  
Ping Wang ◽  
Shihao Zhang ◽  
Xiaozhen Wang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Bactericidal Activity ◽  
Reactive Oxygen ◽  
Oxygen Species
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