scholarly journals Rare‐Cell Enrichment by a Rapid, Label‐Free, Ultrasonic Isopycnic Technique for Medical Diagnostics

2014 ◽  
Vol 126 (22) ◽  
pp. 5693-5696 ◽  
Author(s):  
Yannyk Bourquin ◽  
Abeer Syed ◽  
Julien Reboud ◽  
Lisa C. Ranford‐Cartwright ◽  
Michael P. Barrett ◽  
...  
Keyword(s):  
Medical Diagnostics ◽  
Label Free ◽  
Cell Enrichment

scholarly journals Rare-Cell Enrichment by a Rapid, Label-Free, Ultrasonic Isopycnic Technique for Medical Diagnostics

2014 ◽  
Vol 53 (22) ◽  
pp. 5587-5590 ◽  
Author(s):  
Yannyk Bourquin ◽  
Abeer Syed ◽  
Julien Reboud ◽  
Lisa C. Ranford-Cartwright ◽  
Michael P. Barrett ◽  
...  
Keyword(s):  
Medical Diagnostics ◽  
Label Free ◽  
Cell Enrichment

scholarly journals Immunosensor Based on Long-Period Fiber Gratings for Detection of Viruses Causing Gastroenteritis

Sensors ◽  
2020 ◽  
Vol 20 (3) ◽  
pp. 813 ◽  
Author(s):  
Marta Janczuk-Richter ◽  
Beata Gromadzka ◽  
Łukasz Richter ◽  
Mirosława Panasiuk ◽  
Karolina Zimmer ◽  
...  
Keyword(s):  
Optical Fiber ◽  
Medical Diagnostics ◽  
Label Free ◽  
Fiber Gratings ◽  
Fast Detection ◽  
Long Period ◽  
Highly Sensitive ◽  
Protein Receptors ◽  
Long Period Fiber Gratings ◽  
Vaccine Testing

Since the norovirus is the main cause of acute gastroenteritis all over the world, its fast detection is crucial in medical diagnostics. In this work, a rapid, sensitive, and selective optical fiber biosensor for the detection of norovirus virus-like particles (VLPs) is reported. The sensor is based on highly sensitive long-period fiber gratings (LPFGs) coated with antibodies against the main coat protein of the norovirus. Several modification methods were verified to obtain reliable immobilization of protein receptors on the LPFG surface. We were able to detect 1 ng/mL norovirus VLPs in a 40-min assay in a label-free manner. Thanks to the application of an optical fiber as the sensor, there is a possibility to increase the user’s safety by separating the measurement point from the signal processing setup. Moreover, our sensor is small and light, and the proposed assay is straightforward. The designed LPFG-based biosensor could be applied in both fast norovirus detection and in vaccine testing.

Fabrication of Precise Asymmetric Nanoshells Array with Nanogaps for a Label-Free Immunoassay Based on NIR-Light Responsive LSPR

2012 ◽  
Vol 523-524 ◽  
pp. 680-685
Author(s):  
Shuhei Uchida ◽  
Kazuya Yamamura ◽  
Nobuyuki Zettsu
Keyword(s):  
Near Infrared ◽  
Atmospheric Pressure Plasma ◽  
High Sensitivity ◽  
Medical Diagnostics ◽  
Localized Surface Plasmon ◽  
Label Free ◽  
Fabrication Technique ◽  
Alternative Structures ◽  
Wide Range ◽  
Surrounding Refractive Index

Localized surface plasmon resonance (LSPR) based sensors are a well established technology utilized for label-free biochemical sensing in immunoassay, medical diagnostics and environmental monitoring. The understanding of asymmetric metal nanoparticles, new object for complex, coupled plasmon systems providing localized significantly enhanced E-field, is central to a wide range of novel applications and processes in science of higher sensitive sensing systems. However, few methods are available for actual characterization of such nanostructures at the single particle level. Here we propose a precise and large sized scale fabrication technique for asymmetric nanoshells array with nanogaps of several tens of nanometers for LSPR sensor through atmospheric pressure plasma etching processes. A nanoshell was simply constructed by laminating thin Au films on periodic isolated polymer nanoparticles template. This nanoshells array was expected to exhibit specific near-infrared plasmonic properties. When measuring the sensitivity, nanoshells array exhibited a high sensitivity to changes of surrounding refractive index and showed a higher sensor figure of merit than the alternative structures. This indicated that the enhanced plasmon E-field in the asymmetric nanostructures improved sensor performance. Our fabrication technique and the optical properties of the arrays will provide useful information for developing new plasmonic applications.

scholarly journals Multitarget, quantitative nanoplasmonic electrical field-enhanced resonating device (NE2RD) for diagnostics

2015 ◽  
Vol 112 (32) ◽  
pp. E4354-E4363 ◽  
Author(s):  
Fatih Inci ◽  
Chiara Filippini ◽  
Murat Baday ◽  
Mehmet Ozgun Ozen ◽  
Semih Calamak ◽  
...  
Keyword(s):  
Dynamic Range ◽  
Point Of Care ◽  
Medical Diagnostics ◽  
Clinical Samples ◽  
Label Free ◽  
Protein Biomarkers ◽  
Electrical Field ◽  
Color Changes ◽  
Sensing Platform ◽  
Broad Dynamic Range

Recent advances in biosensing technologies present great potential for medical diagnostics, thus improving clinical decisions. However, creating a label-free general sensing platform capable of detecting multiple biotargets in various clinical specimens over a wide dynamic range, without lengthy sample-processing steps, remains a considerable challenge. In practice, these barriers prevent broad applications in clinics and at patients’ homes. Here, we demonstrate the nanoplasmonic electrical field-enhanced resonating device (NE2RD), which addresses all these impediments on a single platform. The NE2RD employs an immunodetection assay to capture biotargets, and precisely measures spectral color changes by their wavelength and extinction intensity shifts in nanoparticles without prior sample labeling or preprocessing. We present through multiple examples, a label-free, quantitative, portable, multitarget platform by rapidly detecting various protein biomarkers, drugs, protein allergens, bacteria, eukaryotic cells, and distinct viruses. The linear dynamic range of NE2RD is five orders of magnitude broader than ELISA, with a sensitivity down to 400 fg/mL This range and sensitivity are achieved by self-assembling gold nanoparticles to generate hot spots on a 3D-oriented substrate for ultrasensitive measurements. We demonstrate that this precise platform handles multiple clinical samples such as whole blood, serum, and saliva without sample preprocessing under diverse conditions of temperature, pH, and ionic strength. The NE2RD’s broad dynamic range, detection limit, and portability integrated with a disposable fluidic chip have broad applications, potentially enabling the transition toward precision medicine at the point-of-care or primary care settings and at patients’ homes.

Multi-spectral fiber spectroscopy methods as solutions for label-free medical diagnostics (Conference Presentation)

2019 ◽  
Author(s):  
Olga Bibikova ◽  
Ervin Nippolainen ◽  
Isaac Afara ◽  
Julian Haas ◽  
Vesa Virtanen ◽  
...  
Keyword(s):  
Medical Diagnostics ◽  
Label Free

ClearCell FX: A microfluidic system for label-free circulating tumor cell enrichment.

2014 ◽  
Vol 32 (15_suppl) ◽  
pp. e22023-e22023 ◽  
Author(s):  
Andrew Wu ◽  
Ali Asgar Bhagat ◽  
Man Chun Leong ◽  
Chwee Teck Lim
Keyword(s):  
Tumor Cell ◽  
Circulating Tumor Cell ◽  
Microfluidic System ◽  
Label Free ◽  
Cell Enrichment

scholarly journals Nanogravimetric and Optical Characterizations of Thrombin Interaction with a Self-Assembled Thiolated Aptamer

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Jane Politi ◽  
Ilaria Rea ◽  
Fabrizia Nici ◽  
Principia Dardano ◽  
Monica Terracciano ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
High Specificity ◽  
Medical Diagnostics ◽  
Affinity Constant ◽  
Label Free ◽  
Gold Substrate ◽  
System Sensitivity ◽  
All Optical ◽  
Thiolated Aptamer

Efficient biorecognition of thrombin (TB), a serine protease with crucial role in physiological and pathological blood coagulation, is a hot topic in medical diagnostics. In this work, we investigate the ability of synthetic thrombin aptamer (TBA), immobilized on a gold substrate, to bind thrombin by two different label-free techniques: the quartz crystal microbalance (QCM) and the spectroscopic ellipsometry (SE). By QCM characterization in the range from 20 to 110 nM, we demonstrate high specificity of TBA-TB interaction and determine affinity constant (Kd) of17.7±0.3 nM, system sensitivity of0.42±0.03 Hz nM−1, and limit of detection (LOD) of240±20 pM. The interaction between TBA and TB is also investigated by SE, an all-optical method, by quantifying the thickness increase of the TBA film assembled on gold substrate. AFM characterization of TBA and TB molecules deposited on flat silicon surface is also supplied.

scholarly journals Ultrasensitive SERS-Based Plasmonic Sensor with Analyte Enrichment System Produced by Direct Laser Writing

Nanomaterials ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 49 ◽  
Author(s):  
Georgii Pavliuk ◽  
Dmitrii Pavlov ◽  
Eugeny Mitsai ◽  
Oleg Vitrik ◽  
Aleksandr Mironenko ◽  
...  
Keyword(s):  
High Performance ◽  
Organic Dyes ◽  
Medical Diagnostics ◽  
Water Repellent ◽  
Label Free ◽  
Plasmonic Sensor ◽  
Active Sensor ◽  
Direct Laser ◽  
Surface Enhanced Raman ◽  
Analyte Enrichment

We report an easy-to-implement device for surface-enhanced Raman scattering (SERS)-based detection of various analytes dissolved in water droplets at trace concentrations. The device combines an analyte-enrichment system and SERS-active sensor site, both produced via inexpensive and high-performance direct femtosecond (fs)-laser printing. Fabricated on a surface of water-repellent polytetrafluoroethylene substrate as an arrangement of micropillars, the analyte-enrichment system supports evaporating water droplet in the Cassie–Baxter superhydrophobic state, thus ensuring delivery of the dissolved analyte molecules towards the hydrophilic SERS-active site. The efficient pre-concentration of the analyte onto the sensor site based on densely arranged spiky plasmonic nanotextures results in its subsequent label-free identification by means of SERS spectroscopy. Using the proposed device, we demonstrate reliable SERS-based fingerprinting of various analytes, including common organic dyes and medical drugs at ppb concentrations. The proposed device is believed to find applications in various areas, including label-free environmental monitoring, medical diagnostics, and forensics.

scholarly journals Label-Free Optical Resonator-Based Biosensors

Sensors ◽  
2020 ◽  
Vol 20 (20) ◽  
pp. 5901
Author(s):  
Donggee Rho ◽  
Caitlyn Breaux ◽  
Seunghyun Kim
Keyword(s):  
Point Of Care ◽  
Health And Safety ◽  
Disease Diagnosis ◽  
Medical Diagnostics ◽  
Sample Volume ◽  
Small Sample ◽  
Optical Resonator ◽  
Future Research ◽  
Label Free ◽  
Label Free Detection

The demand for biosensor technology has grown drastically over the last few decades, mainly in disease diagnosis, drug development, and environmental health and safety. Optical resonator-based biosensors have been widely exploited to achieve highly sensitive, rapid, and label-free detection of biological analytes. The advancements in microfluidic and micro/nanofabrication technologies allow them to be miniaturized and simultaneously detect various analytes in a small sample volume. By virtue of these advantages and advancements, the optical resonator-based biosensor is considered a promising platform not only for general medical diagnostics but also for point-of-care applications. This review aims to provide an overview of recent progresses in label-free optical resonator-based biosensors published mostly over the last 5 years. We categorized them into Fabry-Perot interferometer-based and whispering gallery mode-based biosensors. The principles behind each biosensor are concisely introduced, and recent progresses in configurations, materials, test setup, and light confinement methods are described. Finally, the current challenges and future research topics of the optical resonator-based biosensor are discussed.

Label-free mesenchymal stem cell enrichment from bone marrow samples by inertial microfluidics

2018 ◽  
Vol 10 (7) ◽  
pp. 713-721 ◽  
Author(s):  
Lap Man Lee ◽  
Jenna M. Rosano ◽  
Yi Wang ◽  
George J. Klarmann ◽  
Charles J. Garson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Bone Marrow Aspirate ◽  
Label Free ◽  
Therapeutic Implications ◽  
Cartilage Reconstruction ◽  
Cell Enrichment ◽  
And Cartilage

Isolation of pure populations of mesenchymal stem cells from bone marrow aspirate is a critical need in regenerative medicine such as orthopedic and cartilage reconstruction with important clinical and therapeutic implications.

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