Innentitelbild: Thiols and Selenols as Electron-Relay Catalysts for Disulfide-Bond Reduction (Angew. Chem. 49/2013)

2013 ◽  
Vol 125 (49) ◽  
pp. 12982-12982 ◽  
Author(s):  
John C. Lukesh ◽  
Brett VanVeller ◽  
Ronald T. Raines
Keyword(s):  
Disulfide Bond ◽  
Disulfide Bond Reduction

scholarly journals Quantitative Risk assessment of product disulfide bond reduction in a recombinant protein manufacturing

2021 ◽  
Author(s):  
Yi Qing cui ◽  
rujie meng ◽  
yutong Li
Keyword(s):  
Risk Assessment ◽  
Cell Culture ◽  
Recombinant Protein ◽  
Disulfide Bond ◽  
Culture Fluid ◽  
Culture Conditions ◽  
Quantitative Risk Assessment ◽  
Prevention Measures ◽  
Recombinant Molecule ◽  
Disulfide Bond Reduction

Disulfide bond reduction occurred now and then during a recombinant protein manufacturing. Their reduction often led in the loss of batches. Various prevention measures were developed. Their implementation in a manufacturing inevitably increases its operation complexity and even might impact its product qualities and process performances. It is highly desirable to apply a reduction measure only if necessary and to apply no strong measure if a moderate one works. The reduction occurrence is determined both by the reduction sensitivity of a recombinant molecule and by the reduction power of harvested cell culture fluids. The reduction power of harvested cell culture fluids varies largely and depends on cells, lysis level, growth stage, and culture conditions. The method of the quantitative risk assessment of disulfide bond reduction was reported here. The quantitative assessment is realized by comparing the reduction sensitivity of a recombinant molecule with the reduction power of its harvested cell culture fluid.

Disulfide bond reduction-triggered molecular hydrogels of folic acid–Taxol conjugates

2013 ◽  
Vol 11 (40) ◽  
pp. 6946 ◽  
Author(s):  
Chengbiao Yang ◽  
Dongxia Li ◽  
Qianqi FengZhao ◽  
Lianyong Wang ◽  
Ling Wang ◽  
...  
Keyword(s):  
Folic Acid ◽  
Disulfide Bond ◽  
Disulfide Bond Reduction

scholarly journals Antibody disulfide bond reduction during process development: Insights using a scale-down model process

2015 ◽  
Vol 9 (S9) ◽  
Author(s):  
Julie Ruaudel ◽  
Martin Bertschinger ◽  
Sonia Letestu ◽  
Roberto Giovannini ◽  
Paul Wassmann ◽  
...  
Keyword(s):  
Disulfide Bond ◽  
Process Development ◽  
Scale Down ◽  
Disulfide Bond Reduction

Mechanism of Integrin Activation by Disulfide Bond Reduction†

Biochemistry ◽  
2001 ◽  
Vol 40 (30) ◽  
pp. 8861-8867 ◽  
Author(s):  
Boxu Yan ◽  
Jeffrey W. Smith
Keyword(s):  
Disulfide Bond ◽  
Integrin Activation ◽  
Disulfide Bond Reduction
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