scholarly journals Titelbild: A Suite of Solid-State NMR Experiments for RNA Intranucleotide Resonance Assignment in a 21 kDa Protein-RNA Complex (Angew. Chem. 38/2013)

2013 ◽  
Vol 125 (38) ◽  
pp. 10045-10045
Author(s):  
Alexander Marchanka ◽  
Bernd Simon ◽  
Teresa Carlomagno
Keyword(s):  
Solid State ◽  
Resonance Assignment ◽  
Solid State Nmr ◽  
Rna Complex

A Suite of Solid-State NMR Experiments for RNA Intranucleotide Resonance Assignment in a 21 kDa Protein-RNA Complex

2013 ◽  
Vol 125 (38) ◽  
pp. 10180-10185 ◽  
Author(s):  
Alexander Marchanka ◽  
Bernd Simon ◽  
Teresa Carlomagno
Keyword(s):  
Solid State ◽  
Resonance Assignment ◽  
Solid State Nmr ◽  
Rna Complex

scholarly journals Resonance assignment of the outer membrane protein AlkL in lipid bilayers by proton-detected solid-state NMR

2020 ◽  
Vol 14 (2) ◽  
pp. 295-300 ◽  
Author(s):  
Tobias Schubeis ◽  
Tom S. Schwarzer ◽  
Tanguy Le Marchand ◽  
Jan Stanek ◽  
Kumar Tekwani Movellan ◽  
...  
Keyword(s):  
Solid State ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Lipid Bilayers ◽  
Resonance Assignment ◽  
Outer Membrane Protein ◽  
Solid State Nmr

scholarly journals Protein resonance assignment by BSH-CP-based 3D solid-state NMR experiments: A practical guide

2019 ◽  
Vol 58 (5) ◽  
pp. 445-465
Author(s):  
Jutta Hoffmann ◽  
Julia Ruta ◽  
Chaowei Shi ◽  
Kitty Hendriks ◽  
Veniamin Chevelkov ◽  
...  
Keyword(s):  
Solid State ◽  
Resonance Assignment ◽  
Solid State Nmr ◽  
Practical Guide ◽  
Protein Resonance Assignment ◽  
3D Solid

scholarly journals 13C Spin Dilution for Simplified and Complete Solid-State NMR Resonance Assignment of Insoluble Biological Assemblies

2011 ◽  
Vol 133 (13) ◽  
pp. 4722-4725 ◽  
Author(s):  
Antoine Loquet ◽  
Guohua Lv ◽  
Karin Giller ◽  
Stefan Becker ◽  
Adam Lange
Keyword(s):  
Solid State ◽  
Resonance Assignment ◽  
Solid State Nmr ◽  
Nmr Resonance Assignment

scholarly journals Protein resonance assignment by solid-state NMR based on 1H-detected 13C-based double-quantum spectroscopy at fast MAS

2021 ◽  
Author(s):  
Alons Lends ◽  
Melanie Berbon ◽  
Birgit Habenstein ◽  
Yusuke Nishiyama ◽  
Antoine Loquet
Keyword(s):  
Solid State ◽  
Resonance Assignment ◽  
Solid State Nmr ◽  
Protein Complexes ◽  
Three Dimensional ◽  
Magic Angle Spinning ◽  
Sequential Assignment ◽  
Double Quantum ◽  
Magic Angle ◽  
Fast Mas

Solid-state NMR spectroscopy is a powerful technique to study insoluble and non-crystalline proteins and protein complexes at atomic resolution. The development of proton (1H) detection at fast magic-angle spinning (MAS) has considerably increased the analytical capabilities of the technique, enabling the acquisition of 1H-detected fingerprint experiments in few hours. Here an approach based on double-quantum (DQ) 13C spectroscopy, detected on 1H, is introduced at fast MAS (70 kHz) to perform the sequential assignment of insoluble proteins of small size, without any specific deuteration requirement. By combining two three-dimensional 1H detected experiments correlating a 13C DQ dimension respectively to its intra-residue and sequential 15N-1H pairs, a sequential walk through DQ (CA+CO) resonance is obtained. Our approach takes advantage of fast MAS to achieve an efficient sensitivity and the addition of a DQ dimension provides spectral features useful for the resonance assignment process.

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